Ultrasound-visible engineered bacteria for tumor chemo-immunotherapy.
Our previous work developed acoustic response bacteria, which enable the precise tuning of transgene expression through ultrasound. However, it is still difficult to visualize these bacteria in order to guide the sound wave to precisely irradiate them. Here, we develop ultrasound-visible engineered bacteria and chemically modify them with doxorubicin (DOX) on their surfaces. These engineered bacteria (Ec@DIG-GVs) can produce gas vesicles (GVs), providing a real-time imaging guide for remote hyperthermia high-intensity focused ultrasound (hHIFU) to induce the expression of the interferon (IFN)-γ gene. The production of IFN-γ can kill tumor cells, induce macrophage polarization from the M2 to the M1 phenotype, and promote the maturation of dendritic cells. DOX can be released in the acidic tumor microenvironment, resulting in immunogenic cell death of tumor cells. The concurrent effects of IFN-γ and DOX activate a tumor-specific T cell response, producing the synergistic anti-tumor efficacy. Our study provides a promising strategy for bacteria-mediated tumor chemo-immunotherapy.
Pubmed ID: 38640931 RIS Download
Research resources used in this publication
Additional research tools detected in this publication
Antibodies used in this publication
- HIF1a antibody (RRID:AB_2882080)
- TruStain FcX(TM) (anti-mouse CD16/32) (RRID:AB_1574975)
- Brilliant Violet 421(TM) anti-mouse FOXP3 (RRID:AB_2565933)
- PE anti-human/mouse Granzyme B Recombinant (RRID:AB_2687031)
- APC anti-mouse TNF-alpha (RRID:AB_315428)
- Brilliant Violet 650(TM) anti-mouse IFN-gamma (RRID:AB_2734492)
- PerCP/Cyanine5.5 anti-mouse CD69 (RRID:AB_2260065)
- Brilliant Violet 421(TM) anti-mouse CD62L (RRID:AB_2562560)
- PE anti-mouse/human CD44 (RRID:AB_493687)
- PE anti-mouse CD279 (PD-1) (RRID:AB_313420)
- PE anti-mouse CD206 (MMR) (RRID:AB_10896421)
- PerCP/Cyanine5.5 anti-mouse F4/80 (RRID:AB_893496)
- APC anti-mouse H-2Kb/H-2Db (RRID:AB_2750194)
- APC anti-mouse CD80 (RRID:AB_313135)
- PerCP/Cyanine5.5 anti-mouse CD86 (RRID:AB_2074994)
- PE anti-mouse CD11c (RRID:AB_313776)
- Alexa Fluor(R) 700 anti-mouse CD4 (RRID:AB_493698)
- FITC anti-mouse CD3 (RRID:AB_312660)
- APC/Fire(TM) 750 anti-mouse CD45 (RRID:AB_2572115)
- Ultra-LEAF(TM) Purified anti-mouse CD274 (B7-H1, PD-L1) (RRID:AB_2800599)
- PE anti-mouse CD366 (Tim-3) (RRID:AB_345378)
- FITC anti-mouse/human CD11b (RRID:AB_312788)
- PE/Cyanine7 anti-mouse CD8a (RRID:AB_312760)
Associated grants
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This is a list of tools and resources that we have found mentioned in this publication.
Addgene (tool)
RRID:SCR_002037
Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
View all literature mentionsFlowJo (tool)
RRID:SCR_008520
Software for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsMendeley Data (tool)
RRID:SCR_015671
Cloud-based data repository for storing, publishing and accessing scientific data. Mendeley Data creates a permanent location and issues Force 11 compliant citations for uploaded data.
View all literature mentionsBALB/cAnNCrl (tool)
RRID:MGI:2683685
laboratory mouse with name BALB/cAnNCrl from MGI.
View all literature mentionsHIF1a antibody (antibody)
RRID:AB_2882080
This monoclonal targets HIF1a
View all literature mentionsTruStain FcX(TM) (anti-mouse CD16/32) (antibody)
RRID:AB_1574975
This monoclonal targets CD16/32
View all literature mentionsBrilliant Violet 421(TM) anti-mouse FOXP3 (antibody)
RRID:AB_2565933
This monoclonal targets FOXP3
View all literature mentionsPE anti-human/mouse Granzyme B Recombinant (antibody)
RRID:AB_2687031
This recombinant monoclonal targets Granzyme B
View all literature mentionsAPC anti-mouse TNF-alpha (antibody)
RRID:AB_315428
This monoclonal targets TNF-alpha
View all literature mentionsBrilliant Violet 650(TM) anti-mouse IFN-gamma (antibody)
RRID:AB_2734492
This monoclonal targets IFN-gamma
View all literature mentionsPerCP/Cyanine5.5 anti-mouse CD69 (antibody)
RRID:AB_2260065
This monoclonal targets CD69
View all literature mentionsBrilliant Violet 421(TM) anti-mouse CD62L (antibody)
RRID:AB_2562560
This monoclonal targets CD62L
View all literature mentionsPE anti-mouse/human CD44 (antibody)
RRID:AB_493687
This monoclonal targets CD44
View all literature mentionsPE anti-mouse CD279 (PD-1) (antibody)
RRID:AB_313420
This monoclonal targets CD279
View all literature mentionsPE anti-mouse CD206 (MMR) (antibody)
RRID:AB_10896421
This monoclonal targets CD206
View all literature mentionsPerCP/Cyanine5.5 anti-mouse F4/80 (antibody)
RRID:AB_893496
This monoclonal targets F4/80
View all literature mentionsAPC anti-mouse H-2Kb/H-2Db (antibody)
RRID:AB_2750194
This monoclonal targets H-2Kb/H-2Db
View all literature mentionsAPC anti-mouse CD80 (antibody)
RRID:AB_313135
This monoclonal targets CD80
View all literature mentionsPerCP/Cyanine5.5 anti-mouse CD86 (antibody)
RRID:AB_2074994
This monoclonal targets CD86
View all literature mentionsPE anti-mouse CD11c (antibody)
RRID:AB_313776
This monoclonal targets CD11c
View all literature mentionsAlexa Fluor(R) 700 anti-mouse CD4 (antibody)
RRID:AB_493698
This monoclonal targets CD4
View all literature mentionsFITC anti-mouse CD3 (antibody)
RRID:AB_312660
This monoclonal targets CD3
View all literature mentionsAPC/Fire(TM) 750 anti-mouse CD45 (antibody)
RRID:AB_2572115
This monoclonal targets CD45
View all literature mentionsUltra-LEAF(TM) Purified anti-mouse CD274 (B7-H1, PD-L1) (antibody)
RRID:AB_2800599
This monoclonal targets CD274
View all literature mentionsPE anti-mouse CD366 (Tim-3) (antibody)
RRID:AB_345378
This monoclonal targets CD366
View all literature mentionsFITC anti-mouse/human CD11b (antibody)
RRID:AB_312788
This monoclonal targets CD11b
View all literature mentionsPE/Cyanine7 anti-mouse CD8a (antibody)
RRID:AB_312760
This monoclonal targets CD8a
View all literature mentions
