Oct4 controls basement membrane development during human embryogenesis.
Basement membranes (BMs) are sheet-like structures of extracellular matrix (ECM) that provide structural support for many tissues and play a central role in signaling. They are key regulators of cell behavior and tissue functions, and defects in their assembly or composition are involved in numerous human diseases. Due to the differences between human and animal embryogenesis, ethical concerns, legal constraints, the scarcity of human tissue material, and the inaccessibility of the in vivo condition, BM regulation during human embryo development has remained elusive. Using the post-implantation amniotic sac embryoid (PASE), we delineate BM assembly upon post-implantation development and BM disassembly during primitive streak (PS) cell dissemination. Further, we show that the transcription factor Oct4 regulates the expression of BM structural components and receptors and controls BM development by regulating Akt signaling and the small GTPase Rac1. These results represent a relevant step toward a more comprehensive understanding of early human development.
Pubmed ID: 38579716
Research resources used in this publication
Additional research tools detected in this publication
None foundAntibodies used in this publication
- IRDye 800CW Goat anti-Mouse IgG (RRID:AB_2687825)
- Anti-rat IgG (H+L), (Alexa Fluor 647 Conjugate) (RRID:AB_1904017)
- Anti-rat IgG (H+L), (Alexa Fluor 488 Conjugate) (RRID:AB_10693769)
- Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor 647 Conjugate) (RRID:AB_10693544)
- Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (RRID:AB_2716249)
- Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) (RRID:AB_1904025)
- Anti-mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor 594 Conjugate) (RRID:AB_2714182)
- Anti-mouse IgG (H+L), F(ab) 2 Fragment (Alexa Fluor 488 Conjugate) (RRID:AB_10694704)
- Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (RRID:AB_2535864)
- Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (RRID:AB_2866497)
- StemLight Pluripotency Transcription Factor Antibody Kit (RRID:AB_10697029)
- IRDye 800CW Goat anti-Rabbit IgG (RRID:AB_2651127)
- StemLight Pluripotency Surface Marker Antibody Kit (RRID:AB_10693335)
- Goat Anti-Human Nidogen-2 Polyclonal antibody, Unconjugated (RRID:AB_2153427)
- Snail (C15D3) Rabbit mAb (RRID:AB_2255011)
- Oct-4A (C30A3) Rabbit Antibody (RRID:AB_2167691)
- Oct-4 (D7O5Z) Mouse mAb (RRID:AB_2799870)
- Goat Anti-Human Oct-3 / 4 Polyclonal antibody, Unconjugated (RRID:AB_354975)
- Laminin alpha 5 antibody [4B12] (RRID:AB_1566369)
- Rabbit Anti-Laminin Polyclonal Antibody, Unconjugated (RRID:AB_298179)
- Anti-Integrin beta1 (CD29) Antibody, clone mAb13 (RRID:AB_2891299)
- Purified anti-human/mouse CD49f (RRID:AB_345296)
- Mouse Anti-Human Nidogen-1 / entactin Monoclonal antibody, Unconjugated, Clone 302117 (RRID:AB_2153270)
- Anti-Laminin gamma1, clone A5 (RRID:AB_2134338)
- Anti-Heparan Sulfate Proteoglycan, Perlecan, clone A7L6 (RRID:AB_2119239)
- Phospho-Histone H3 (Ser10) Antibody (RRID:AB_331535)
- GATA-3 (D13C9) XP Rabbit mAb (RRID:AB_10835690)
- Rabbit Anti-Human GAPDH Polyclonal Antibody, Unconjugated (RRID:AB_2107456)
- Mouse Anti-Ezrin Monoclonal Antibody, Unconjugated, Clone 3C12 (RRID:AB_304261)
- Mouse Anti-Laminin S Monoclonal antibody, Unconjugated, Clone c4 (RRID:AB_2134201)
- Purified Mouse Anti-E-cadherin (RRID:AB_397580)
- Rabbit Anti-Collagen IV Polyclonal Antibody, Unconjugated (RRID:AB_305584)
- Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (RRID:AB_2070042)
- Brachyury (D2Z3J) Rabbit mAb (RRID:AB_2799983)
- Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (RRID:AB_2315049)
- β-Catenin (D10A8) XP® Rabbit mAb (RRID:AB_11127855)
- Anti-alpha-Tubulin Mouse mAb (DM1A) (RRID:AB_2617116)
- Mouse Anti-Dystroglycan, alpha Monoclonal antibody, Unconjugated, Clone iih6c4 (RRID:AB_309828)
Associated grants
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This is a list of tools and resources that we have found mentioned in this publication.
IRDye 800CW Goat anti-Mouse IgG (antibody)
RRID:AB_2687825
This polyclonal targets IgG
View all literature mentionsAnti-rat IgG (H+L), (Alexa Fluor 647 Conjugate) (antibody)
RRID:AB_1904017
This unknown targets rat IgG (H+L) (Alexa Fluor 647 Conjugate)
View all literature mentionsAnti-rat IgG (H+L), (Alexa Fluor 488 Conjugate) (antibody)
RRID:AB_10693769
This unknown targets rat IgG (H+L) (Alexa Fluor 488 Conjugate)
View all literature mentionsAnti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor 647 Conjugate) (antibody)
RRID:AB_10693544
This unknown targets IgG (H+L)
View all literature mentionsAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (antibody)
RRID:AB_2716249
This polyclonal targets Rabbit IgG
View all literature mentionsAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) (antibody)
RRID:AB_1904025
This unknown targets IgG (H+L)
View all literature mentionsAnti-mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor 594 Conjugate) (antibody)
RRID:AB_2714182
This unknown targets Mouse IgG
View all literature mentionsAnti-mouse IgG (H+L), F(ab) 2 Fragment (Alexa Fluor 488 Conjugate) (antibody)
RRID:AB_10694704
This unknown targets IgG (H+L)
View all literature mentionsDonkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (antibody)
RRID:AB_2535864
This polyclonal secondary targets IgG (H+L)
View all literature mentionsDonkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (antibody)
RRID:AB_2866497
This polyclonal secondary targets IgG (H+L)
View all literature mentionsStemLight Pluripotency Transcription Factor Antibody Kit (antibody)
RRID:AB_10697029
This unknown targets StemLight Pluripotency Transcription Factor Kit
View all literature mentionsIRDye 800CW Goat anti-Rabbit IgG (antibody)
RRID:AB_2651127
This polyclonal targets IgG
View all literature mentionsStemLight Pluripotency Surface Marker Antibody Kit (antibody)
RRID:AB_10693335
This unknown targets StemLight Pluripotency Surface Marker Kit
View all literature mentionsGoat Anti-Human Nidogen-2 Polyclonal antibody, Unconjugated (antibody)
RRID:AB_2153427
This polyclonal targets Human Nidogen-2
View all literature mentionsSnail (C15D3) Rabbit mAb (antibody)
RRID:AB_2255011
This monoclonal targets Snai1
View all literature mentionsOct-4A (C30A3) Rabbit Antibody (antibody)
RRID:AB_2167691
This monoclonal targets Oct-4A (C30A3)
View all literature mentionsOct-4 (D7O5Z) Mouse mAb (antibody)
RRID:AB_2799870
This monoclonal targets Oct4
View all literature mentionsGoat Anti-Human Oct-3 / 4 Polyclonal antibody, Unconjugated (antibody)
RRID:AB_354975
This polyclonal targets Human Oct-3 / 4
View all literature mentionsLaminin alpha 5 antibody [4B12] (antibody)
RRID:AB_1566369
This monoclonal targets Human Laminin alpha 5
View all literature mentionsRabbit Anti-Laminin Polyclonal Antibody, Unconjugated (antibody)
RRID:AB_298179
This polyclonal targets Laminin
View all literature mentionsAnti-Integrin beta1 (CD29) Antibody, clone mAb13 (antibody)
RRID:AB_2891299
This monoclonal targets β1-integrin
View all literature mentionsPurified anti-human/mouse CD49f (antibody)
RRID:AB_345296
This monoclonal targets CD49f
View all literature mentionsMouse Anti-Human Nidogen-1 / entactin Monoclonal antibody, Unconjugated, Clone 302117 (antibody)
RRID:AB_2153270
This monoclonal targets Human Nidogen-1 / Entactin
View all literature mentionsAnti-Laminin gamma1, clone A5 (antibody)
RRID:AB_2134338
This monoclonal targets Lamc1
View all literature mentionsAnti-Heparan Sulfate Proteoglycan, Perlecan, clone A7L6 (antibody)
RRID:AB_2119239
This monoclonal targets Hspg2
View all literature mentionsPhospho-Histone H3 (Ser10) Antibody (antibody)
RRID:AB_331535
This polyclonal targets Phospho-Histone H3 (Ser10)
View all literature mentionsGATA-3 (D13C9) XP Rabbit mAb (antibody)
RRID:AB_10835690
This monoclonal targets GATA-3 (D13C9) XP Rabbit mAb
View all literature mentionsRabbit Anti-Human GAPDH Polyclonal Antibody, Unconjugated (antibody)
RRID:AB_2107456
This polyclonal targets Human GAPDH
View all literature mentionsMouse Anti-Ezrin Monoclonal Antibody, Unconjugated, Clone 3C12 (antibody)
RRID:AB_304261
This monoclonal targets Ezrin
View all literature mentionsMouse Anti-Laminin S Monoclonal antibody, Unconjugated, Clone c4 (antibody)
RRID:AB_2134201
This monoclonal targets Laminin S
View all literature mentionsPurified Mouse Anti-E-cadherin (antibody)
RRID:AB_397580
This monoclonal targets E-cadherin
View all literature mentionsRabbit Anti-Collagen IV Polyclonal Antibody, Unconjugated (antibody)
RRID:AB_305584
This polyclonal targets Collagen IV
View all literature mentionsCleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (antibody)
RRID:AB_2070042
This monoclonal targets Casp3
View all literature mentionsBrachyury (D2Z3J) Rabbit mAb (antibody)
RRID:AB_2799983
This monoclonal targets Brachyury
View all literature mentionsPhospho-Akt (Ser473) (D9E) XP® Rabbit mAb (antibody)
RRID:AB_2315049
This monoclonal targets Phospho-Akt (Ser473)
View all literature mentionsβ-Catenin (D10A8) XP® Rabbit mAb (antibody)
RRID:AB_11127855
This monoclonal targets β-Catenin
View all literature mentionsAnti-alpha-Tubulin Mouse mAb (DM1A) (antibody)
RRID:AB_2617116
This monoclonal targets alpha-Tubulin
View all literature mentionsMouse Anti-Dystroglycan, alpha Monoclonal antibody, Unconjugated, Clone iih6c4 (antibody)
RRID:AB_309828
This monoclonal targets Dystroglycan, alpha
View all literature mentionsWA19 (cell line)
RRID:CVCL_9780
Cell line WA19 is a Embryonic stem cell with a species of origin Homo sapiens
View all literature mentions
