Bile acids modified by the intestinal microbiota promote colorectal cancer growth by suppressing CD8+ T cell effector functions.
Concentrations of the secondary bile acid, deoxycholic acid (DCA), are aberrantly elevated in colorectal cancer (CRC) patients, but the consequences remain poorly understood. Here, we screened a library of gut microbiota-derived metabolites and identified DCA as a negative regulator for CD8+ T cell effector function. Mechanistically, DCA suppressed CD8+ T cell responses by targeting plasma membrane Ca2+ ATPase (PMCA) to inhibit Ca2+-nuclear factor of activated T cells (NFAT)2 signaling. In CRC patients, CD8+ T cell effector function negatively correlated with both DCA concentration and expression of a bacterial DCA biosynthetic gene. Bacteria harboring DCA biosynthetic genes suppressed CD8+ T cells effector function and promoted tumor growth in mice. This effect was abolished by disrupting bile acid metabolism via bile acid chelation, genetic ablation of bacterial DCA biosynthetic pathway, or specific bacteriophage. Our study demonstrated causation between microbial DCA metabolism and anti-tumor CD8+ T cell response in CRC, suggesting potential directions for anti-tumor therapy.
Pubmed ID: 38479384 RIS Download
Research resources used in this publication
- pMD2.G (RRID:Addgene_12259) (plasmid)
- psPAX2 (RRID:Addgene_12260) (plasmid)
- C57BL/6JGpt-Rag1em1Cd3259/Gpt (RRID:IMSR_GPT:T004753) (organism)
- C57BL/6JGpt (RRID:IMSR_GPT:N000013) (organism)
- STOCK Nr1h4tm1Gonz/J (RRID:IMSR_JAX:004144) (organism)
- HEK293T (RRID:CVCL_0063) (cell line)
- B6.129S4-Vdrtm1Mbd/J (RRID:IMSR_JAX:006133) (organism)
- C57BL/6N-Gpbar1tm1(KOMP)Vlcg/MbpMmucd (RRID:MMRRC_047915-UCD) (organism)
- B6.129S4-Vdrtm1Mbd/J (RRID:IMSR_JAX:006133) (organism)
- C57BL/6N-Gpbar1tm1(KOMP)Vlcg/MbpMmucd (RRID:MMRRC_047915-UCD) (organism)
- Anti-TNF-alpha Rabbit pAb (RRID:AB_3075517) (antibody)
- Interferon gamma antibody (RRID:AB_448613) (antibody)
- InVivoPlus anti-mouse CD8α (RRID:AB_1107671) (antibody)
- Granzyme B Antibody (RRID:AB_3075510) (antibody)
- HRP-labeled Goat Anti-Rabbit IgG (H+L) (RRID:AB_2892644) (antibody)
- Horseradish-labeled goat anti-mouse IgG (H+L) antibody (RRID:AB_2860575) (antibody)
- PMCA1 Antibody (5F10) (RRID:AB_2091527) (antibody)
- PMCA4 Antibody (RRID:AB_2061726) (antibody)
- GAPDH antibody (RRID:AB_2107436) (antibody)
- Lamin B1 antibody (RRID:AB_11232208) (antibody)
- Phospho-PLCgamma1 (Tyr783) Antibody (RRID:AB_330855) (antibody)
- Phospho-LAT (Tyr191) Antibody (RRID:AB_2157728) (antibody)
- Phospho-Src Family (Tyr416) Antibody (RRID:AB_331697) (antibody)
- Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb (RRID:AB_2218658) (antibody)
- Monoclonal ANTI-FLAG® M2 antibody produced in mouse (RRID:AB_262044) (antibody)
- Beta Actin antibody (RRID:AB_2687938) (antibody)
- Mouse Anti-NFAT2 Monoclonal Antibody, Unconjugated, Clone 7A6 (RRID:AB_303308) (antibody)
- Ultra-LEAF(TM) Purified anti-human CD3 (RRID:AB_11146991) (antibody)
- Ultra-LEAF(TM) Purified anti-mouse IFN-gamma (RRID:AB_2616675) (antibody)
- Ultra-LEAF(TM) Purified anti-mouse IL-4 (RRID:AB_2750404) (antibody)
- Ultra-LEAF(TM) Purified anti-human CD28 (RRID:AB_11148949) (antibody)
- Ultra-LEAF(TM) Purified anti-mouse CD3 (RRID:AB_2810314) (antibody)
- Granzyme B (RRID:AB_2738175) (antibody)
- FITC anti-human IFN-gamma (RRID:AB_315437) (antibody)
- Mouse Anti-TNF Monoclonal Antibody, PE-Cy7 Conjugated, Clone MAb11 (RRID:AB_396764) (antibody)
- PE anti-human TNF-alpha (RRID:AB_315260) (antibody)
- Brilliant Violet 421(TM) anti-mouse TCR beta chain (RRID:AB_2562562) (antibody)
- Brilliant Violet 421(TM) anti-mouse CD366 (Tim-3) (RRID:AB_2616908) (antibody)
- CD3e (RRID:AB_11153670) (antibody)
- Pacific Blue(TM) anti-mouse CD3 (RRID:AB_493644) (antibody)
- APC/Cyanine7 anti-mouse CD8a (RRID:AB_312752) (antibody)
- APC anti-mouse CD25 (RRID:AB_312860) (antibody)
- APC anti-mouse TNF-alpha (RRID:AB_315428) (antibody)
- APC anti-mouse CD279 (PD-1) (RRID:AB_2159183) (antibody)
- PE/Cyanine7 anti-mouse CD3 (RRID:AB_1732057) (antibody)
- PE/Cyanine7 anti-mouse TCR beta chain (RRID:AB_893625) (antibody)
- PE/Cyanine7 anti-mouse IFN-gamma (RRID:AB_2295770) (antibody)
- PerCP anti-mouse CD45 (RRID:AB_893339) (antibody)
- PE/Cyanine5 anti-human CD8a (RRID:AB_314113) (antibody)
- PerCP/Cyanine5.5 anti-mouse CD69 (RRID:AB_2260065) (antibody)
- PerCP/Cyanine5.5 anti-mouse CD4 (RRID:AB_893324) (antibody)
- TOX Monoclonal Antibody (TXRX10), eFluor™ 660, eBioscience (RRID:AB_2574265) (antibody)
- TOX Monoclonal Antibody (TXRX10), PE, eBioscience (RRID:AB_10855034) (antibody)
- PE anti-mouse CD279 (PD-1) (RRID:AB_313421) (antibody)
- PE anti-human/mouse Granzyme B Recombinant (RRID:AB_2687031) (antibody)
- PE anti-mouse Ki-67 (RRID:AB_2561525) (antibody)
- FITC anti-mouse CD4 (RRID:AB_312691) (antibody)
- FITC anti-mouse/human CD44 (RRID:AB_493684) (antibody)
- FITC anti-mouse CD45.2 (RRID:AB_313442) (antibody)
Additional research tools detected in this publication
None foundAntibodies used in this publication
- Anti-TNF-alpha Rabbit pAb (RRID:AB_3075517)
- Interferon gamma antibody (RRID:AB_448613)
- Granzyme B Antibody (RRID:AB_3075510)
- InVivoPlus anti-mouse CD8α (RRID:AB_1107671)
- HRP-labeled Goat Anti-Rabbit IgG (H+L) (RRID:AB_2892644)
- Horseradish-labeled goat anti-mouse IgG (H+L) antibody (RRID:AB_2860575)
- PMCA1 Antibody (5F10) (RRID:AB_2091527)
- PMCA4 Antibody (RRID:AB_2061726)
- GAPDH antibody (RRID:AB_2107436)
- Lamin B1 antibody (RRID:AB_11232208)
- Phospho-PLCgamma1 (Tyr783) Antibody (RRID:AB_330855)
- Phospho-LAT (Tyr191) Antibody (RRID:AB_2157728)
- Phospho-Src Family (Tyr416) Antibody (RRID:AB_331697)
- Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb (RRID:AB_2218658)
- Monoclonal ANTI-FLAG® M2 antibody produced in mouse (RRID:AB_262044)
- Beta Actin antibody (RRID:AB_2687938)
- Mouse Anti-NFAT2 Monoclonal Antibody, Unconjugated, Clone 7A6 (RRID:AB_303308)
- Ultra-LEAF(TM) Purified anti-human CD3 (RRID:AB_11146991)
- Ultra-LEAF(TM) Purified anti-mouse IFN-gamma (RRID:AB_2616675)
- Ultra-LEAF(TM) Purified anti-mouse IL-4 (RRID:AB_2750404)
- Ultra-LEAF(TM) Purified anti-human CD28 (RRID:AB_11148949)
- Ultra-LEAF(TM) Purified anti-mouse CD3 (RRID:AB_2810314)
- Granzyme B (RRID:AB_2738175)
- FITC anti-human IFN-gamma (RRID:AB_315437)
- Mouse Anti-TNF Monoclonal Antibody, PE-Cy7 Conjugated, Clone MAb11 (RRID:AB_396764)
- PE anti-human TNF-alpha (RRID:AB_315260)
- Brilliant Violet 421(TM) anti-mouse TCR beta chain (RRID:AB_2562562)
- Brilliant Violet 421(TM) anti-mouse CD366 (Tim-3) (RRID:AB_2616908)
- CD3e (RRID:AB_11153670)
- Pacific Blue(TM) anti-mouse CD3 (RRID:AB_493644)
- APC/Cyanine7 anti-mouse CD8a (RRID:AB_312752)
- APC anti-mouse CD25 (RRID:AB_312860)
- APC anti-mouse TNF-alpha (RRID:AB_315428)
- APC anti-mouse CD279 (PD-1) (RRID:AB_2159183)
- PE/Cyanine7 anti-mouse CD3 (RRID:AB_1732057)
- PE/Cyanine7 anti-mouse TCR beta chain (RRID:AB_893625)
- PE/Cyanine7 anti-mouse IFN-gamma (RRID:AB_2295770)
- PerCP anti-mouse CD45 (RRID:AB_893339)
- PE/Cyanine5 anti-human CD8a (RRID:AB_314113)
- PerCP/Cyanine5.5 anti-mouse CD69 (RRID:AB_2260065)
- PerCP/Cyanine5.5 anti-mouse CD4 (RRID:AB_893324)
- TOX Monoclonal Antibody (TXRX10), eFluor™ 660, eBioscience (RRID:AB_2574265)
- TOX Monoclonal Antibody (TXRX10), PE, eBioscience (RRID:AB_10855034)
- PE anti-mouse CD279 (PD-1) (RRID:AB_313421)
- PE anti-human/mouse Granzyme B Recombinant (RRID:AB_2687031)
- PE anti-mouse Ki-67 (RRID:AB_2561525)
- FITC anti-mouse CD4 (RRID:AB_312691)
- FITC anti-mouse/human CD44 (RRID:AB_493684)
- FITC anti-mouse CD45.2 (RRID:AB_313442)
Associated grants
NonePublication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This is a list of tools and resources that we have found mentioned in this publication.
Anti-TNF-alpha Rabbit pAb (antibody)
RRID:AB_3075517
This polyclonal targets TNF-alpha
View all literature mentionsInterferon gamma antibody (antibody)
RRID:AB_448613
This polyclonal targets Interferon gamma antibody
View all literature mentionsGranzyme B Antibody (antibody)
RRID:AB_3075510
This monoclonal targets Granzyme B
View all literature mentionsInVivoPlus anti-mouse CD8α (antibody)
RRID:AB_1107671
This monoclonal targets CD8α
View all literature mentionsHRP-labeled Goat Anti-Rabbit IgG (H+L) (antibody)
RRID:AB_2892644
This polyclonal secondary targets IgG (H+L)
View all literature mentionsHorseradish-labeled goat anti-mouse IgG (H+L) antibody (antibody)
RRID:AB_2860575
This polyclonal targets IgG (H+L)
View all literature mentionsPMCA1 Antibody (5F10) (antibody)
RRID:AB_2091527
This monoclonal targets PMCA1
View all literature mentionsPMCA4 Antibody (antibody)
RRID:AB_2061726
This monoclonal targets PMCA4
View all literature mentionsGAPDH antibody (antibody)
RRID:AB_2107436
This monoclonal targets GAPDH
View all literature mentionsLamin B1 antibody (antibody)
RRID:AB_11232208
This monoclonal targets Lamin B1
View all literature mentionsPhospho-PLCgamma1 (Tyr783) Antibody (antibody)
RRID:AB_330855
This polyclonal targets Phospho-PLCgamma1 (Tyr783)
View all literature mentionsPhospho-LAT (Tyr191) Antibody (antibody)
RRID:AB_2157728
This unknown targets LAT, phospho (Tyr191)
View all literature mentionsPhospho-Src Family (Tyr416) Antibody (antibody)
RRID:AB_331697
This polyclonal targets Phospho-Src Family (Tyr416)
View all literature mentionsPhospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb (antibody)
RRID:AB_2218658
This monoclonal targets Phospho-Zap-70 (Tyr319)/Syk (Tyr352)
View all literature mentionsMonoclonal ANTI-FLAG® M2 antibody produced in mouse (antibody)
RRID:AB_262044
This monoclonal targets FLAG
View all literature mentionsBeta Actin antibody (antibody)
RRID:AB_2687938
This monoclonal targets Beta Actin
View all literature mentionsMouse Anti-NFAT2 Monoclonal Antibody, Unconjugated, Clone 7A6 (antibody)
RRID:AB_303308
This monoclonal targets NFAT2
View all literature mentionsUltra-LEAF(TM) Purified anti-human CD3 (antibody)
RRID:AB_11146991
This monoclonal targets CD3
View all literature mentionsUltra-LEAF(TM) Purified anti-mouse IFN-gamma (antibody)
RRID:AB_2616675
This monoclonal targets IFN-gamma
View all literature mentionsUltra-LEAF(TM) Purified anti-mouse IL-4 (antibody)
RRID:AB_2750404
This monoclonal targets IL-4
View all literature mentionsUltra-LEAF(TM) Purified anti-human CD28 (antibody)
RRID:AB_11148949
This monoclonal targets CD28
View all literature mentionsUltra-LEAF(TM) Purified anti-mouse CD3 (antibody)
RRID:AB_2810314
This monoclonal targets CD3
View all literature mentionsGranzyme B (antibody)
RRID:AB_2738175
This monoclonal targets Granzyme B
View all literature mentionsFITC anti-human IFN-gamma (antibody)
RRID:AB_315437
This monoclonal targets IFN-gamma
View all literature mentionsMouse Anti-TNF Monoclonal Antibody, PE-Cy7 Conjugated, Clone MAb11 (antibody)
RRID:AB_396764
This monoclonal targets TNF
View all literature mentionsPE anti-human TNF-alpha (antibody)
RRID:AB_315260
This monoclonal targets TNF-alpha
View all literature mentionsBrilliant Violet 421(TM) anti-mouse TCR beta chain (antibody)
RRID:AB_2562562
This monoclonal targets TCR beta chain
View all literature mentionsBrilliant Violet 421(TM) anti-mouse CD366 (Tim-3) (antibody)
RRID:AB_2616908
This monoclonal targets CD366
View all literature mentionsPacific Blue(TM) anti-mouse CD3 (antibody)
RRID:AB_493644
This monoclonal targets CD3
View all literature mentionsAPC/Cyanine7 anti-mouse CD8a (antibody)
RRID:AB_312752
This monoclonal targets CD8a
View all literature mentionsAPC anti-mouse CD25 (antibody)
RRID:AB_312860
This monoclonal targets CD25
View all literature mentionsAPC anti-mouse TNF-alpha (antibody)
RRID:AB_315428
This monoclonal targets TNF-alpha
View all literature mentionsAPC anti-mouse CD279 (PD-1) (antibody)
RRID:AB_2159183
This monoclonal targets CD279
View all literature mentionsPE/Cyanine7 anti-mouse CD3 (antibody)
RRID:AB_1732057
This monoclonal targets CD3
View all literature mentionsPE/Cyanine7 anti-mouse TCR beta chain (antibody)
RRID:AB_893625
This monoclonal targets TCR beta chain
View all literature mentionsPE/Cyanine7 anti-mouse IFN-gamma (antibody)
RRID:AB_2295770
This monoclonal targets IFN-gamma
View all literature mentionsPerCP anti-mouse CD45 (antibody)
RRID:AB_893339
This monoclonal targets CD45
View all literature mentionsPE/Cyanine5 anti-human CD8a (antibody)
RRID:AB_314113
This monoclonal targets CD8a
View all literature mentionsPerCP/Cyanine5.5 anti-mouse CD69 (antibody)
RRID:AB_2260065
This monoclonal targets CD69
View all literature mentionsPerCP/Cyanine5.5 anti-mouse CD4 (antibody)
RRID:AB_893324
This monoclonal targets CD4
View all literature mentionsTOX Monoclonal Antibody (TXRX10), eFluor™ 660, eBioscience (antibody)
RRID:AB_2574265
This monoclonal targets TOX
View all literature mentionsTOX Monoclonal Antibody (TXRX10), PE, eBioscience (antibody)
RRID:AB_10855034
This monoclonal targets TOX
View all literature mentionsPE anti-mouse CD279 (PD-1) (antibody)
RRID:AB_313421
This monoclonal targets CD279
View all literature mentionsPE anti-human/mouse Granzyme B Recombinant (antibody)
RRID:AB_2687031
This recombinant monoclonal targets Granzyme B
View all literature mentionsPE anti-mouse Ki-67 (antibody)
RRID:AB_2561525
This monoclonal targets Ki-67
View all literature mentionsFITC anti-mouse CD4 (antibody)
RRID:AB_312691
This monoclonal targets CD4
View all literature mentionsFITC anti-mouse/human CD44 (antibody)
RRID:AB_493684
This monoclonal targets CD44
View all literature mentionsFITC anti-mouse CD45.2 (antibody)
RRID:AB_313442
This monoclonal targets CD45.2
View all literature mentionsC57BL/6JGpt-Rag1em1Cd3259/Gpt (organism)
RRID:IMSR_GPT:T004753
Mus musculus with name C57BL/6JGpt-Rag1em1Cd3259/Gpt from IMSR.
View all literature mentionsC57BL/6JGpt (organism)
RRID:IMSR_GPT:N000013
Mus musculus with name C57BL/6JGpt from IMSR.
View all literature mentionsSTOCK Nr1h4tm1Gonz/J (organism)
RRID:IMSR_JAX:004144
Mus musculus with name STOCK Nr1h4tm1Gonz/J from IMSR.
View all literature mentionsHEK293T (cell line)
RRID:CVCL_0063
Cell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsB6.129S4-Vdrtm1Mbd/J (organism)
RRID:IMSR_JAX:006133
Mus musculus with name B6.129S4-Vdrtm1Mbd/J from IMSR.
View all literature mentionsC57BL/6N-Gpbar1tm1(KOMP)Vlcg/MbpMmucd (organism)
RRID:MMRRC_047915-UCD
Mus musculus with name C57BL/6N-Gpbar1tm1(KOMP)Vlcg/MbpMmucd from MMRRC.
View all literature mentionsB6.129S4-Vdrtm1Mbd/J (organism)
RRID:IMSR_JAX:006133
Mus musculus with name B6.129S4-Vdrtm1Mbd/J from IMSR.
View all literature mentionsC57BL/6N-Gpbar1tm1(KOMP)Vlcg/MbpMmucd (organism)
RRID:MMRRC_047915-UCD
Mus musculus with name C57BL/6N-Gpbar1tm1(KOMP)Vlcg/MbpMmucd from MMRRC.
View all literature mentionsAnti-TNF-alpha Rabbit pAb (antibody)
RRID:AB_3075517
This polyclonal targets TNF-alpha
View all literature mentionsInterferon gamma antibody (antibody)
RRID:AB_448613
This polyclonal targets Interferon gamma antibody
View all literature mentionsInVivoPlus anti-mouse CD8α (antibody)
RRID:AB_1107671
This monoclonal targets CD8α
View all literature mentionsGranzyme B Antibody (antibody)
RRID:AB_3075510
This monoclonal targets Granzyme B
View all literature mentionsHRP-labeled Goat Anti-Rabbit IgG (H+L) (antibody)
RRID:AB_2892644
This polyclonal secondary targets IgG (H+L)
View all literature mentionsHorseradish-labeled goat anti-mouse IgG (H+L) antibody (antibody)
RRID:AB_2860575
This polyclonal targets IgG (H+L)
View all literature mentionsPMCA1 Antibody (5F10) (antibody)
RRID:AB_2091527
This monoclonal targets PMCA1
View all literature mentionsPMCA4 Antibody (antibody)
RRID:AB_2061726
This monoclonal targets PMCA4
View all literature mentionsGAPDH antibody (antibody)
RRID:AB_2107436
This monoclonal targets GAPDH
View all literature mentionsLamin B1 antibody (antibody)
RRID:AB_11232208
This monoclonal targets Lamin B1
View all literature mentionsPhospho-PLCgamma1 (Tyr783) Antibody (antibody)
RRID:AB_330855
This polyclonal targets Phospho-PLCgamma1 (Tyr783)
View all literature mentionsPhospho-LAT (Tyr191) Antibody (antibody)
RRID:AB_2157728
This unknown targets LAT, phospho (Tyr191)
View all literature mentionsPhospho-Src Family (Tyr416) Antibody (antibody)
RRID:AB_331697
This polyclonal targets Phospho-Src Family (Tyr416)
View all literature mentionsPhospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb (antibody)
RRID:AB_2218658
This monoclonal targets Phospho-Zap-70 (Tyr319)/Syk (Tyr352)
View all literature mentionsMonoclonal ANTI-FLAG® M2 antibody produced in mouse (antibody)
RRID:AB_262044
This monoclonal targets FLAG
View all literature mentionsBeta Actin antibody (antibody)
RRID:AB_2687938
This monoclonal targets Beta Actin
View all literature mentionsMouse Anti-NFAT2 Monoclonal Antibody, Unconjugated, Clone 7A6 (antibody)
RRID:AB_303308
This monoclonal targets NFAT2
View all literature mentionsUltra-LEAF(TM) Purified anti-human CD3 (antibody)
RRID:AB_11146991
This monoclonal targets CD3
View all literature mentionsUltra-LEAF(TM) Purified anti-mouse IFN-gamma (antibody)
RRID:AB_2616675
This monoclonal targets IFN-gamma
View all literature mentionsUltra-LEAF(TM) Purified anti-mouse IL-4 (antibody)
RRID:AB_2750404
This monoclonal targets IL-4
View all literature mentionsUltra-LEAF(TM) Purified anti-human CD28 (antibody)
RRID:AB_11148949
This monoclonal targets CD28
View all literature mentionsUltra-LEAF(TM) Purified anti-mouse CD3 (antibody)
RRID:AB_2810314
This monoclonal targets CD3
View all literature mentionsGranzyme B (antibody)
RRID:AB_2738175
This monoclonal targets Granzyme B
View all literature mentionsFITC anti-human IFN-gamma (antibody)
RRID:AB_315437
This monoclonal targets IFN-gamma
View all literature mentionsMouse Anti-TNF Monoclonal Antibody, PE-Cy7 Conjugated, Clone MAb11 (antibody)
RRID:AB_396764
This monoclonal targets TNF
View all literature mentionsPE anti-human TNF-alpha (antibody)
RRID:AB_315260
This monoclonal targets TNF-alpha
View all literature mentionsBrilliant Violet 421(TM) anti-mouse TCR beta chain (antibody)
RRID:AB_2562562
This monoclonal targets TCR beta chain
View all literature mentionsBrilliant Violet 421(TM) anti-mouse CD366 (Tim-3) (antibody)
RRID:AB_2616908
This monoclonal targets CD366
View all literature mentionsPacific Blue(TM) anti-mouse CD3 (antibody)
RRID:AB_493644
This monoclonal targets CD3
View all literature mentionsAPC/Cyanine7 anti-mouse CD8a (antibody)
RRID:AB_312752
This monoclonal targets CD8a
View all literature mentionsAPC anti-mouse CD25 (antibody)
RRID:AB_312860
This monoclonal targets CD25
View all literature mentionsAPC anti-mouse TNF-alpha (antibody)
RRID:AB_315428
This monoclonal targets TNF-alpha
View all literature mentionsAPC anti-mouse CD279 (PD-1) (antibody)
RRID:AB_2159183
This monoclonal targets CD279
View all literature mentionsPE/Cyanine7 anti-mouse CD3 (antibody)
RRID:AB_1732057
This monoclonal targets CD3
View all literature mentionsPE/Cyanine7 anti-mouse TCR beta chain (antibody)
RRID:AB_893625
This monoclonal targets TCR beta chain
View all literature mentionsPE/Cyanine7 anti-mouse IFN-gamma (antibody)
RRID:AB_2295770
This monoclonal targets IFN-gamma
View all literature mentionsPerCP anti-mouse CD45 (antibody)
RRID:AB_893339
This monoclonal targets CD45
View all literature mentionsPE/Cyanine5 anti-human CD8a (antibody)
RRID:AB_314113
This monoclonal targets CD8a
View all literature mentionsPerCP/Cyanine5.5 anti-mouse CD69 (antibody)
RRID:AB_2260065
This monoclonal targets CD69
View all literature mentionsPerCP/Cyanine5.5 anti-mouse CD4 (antibody)
RRID:AB_893324
This monoclonal targets CD4
View all literature mentionsTOX Monoclonal Antibody (TXRX10), eFluor™ 660, eBioscience (antibody)
RRID:AB_2574265
This monoclonal targets TOX
View all literature mentionsTOX Monoclonal Antibody (TXRX10), PE, eBioscience (antibody)
RRID:AB_10855034
This monoclonal targets TOX
View all literature mentionsPE anti-mouse CD279 (PD-1) (antibody)
RRID:AB_313421
This monoclonal targets CD279
View all literature mentionsPE anti-human/mouse Granzyme B Recombinant (antibody)
RRID:AB_2687031
This recombinant monoclonal targets Granzyme B
View all literature mentionsPE anti-mouse Ki-67 (antibody)
RRID:AB_2561525
This monoclonal targets Ki-67
View all literature mentionsFITC anti-mouse CD4 (antibody)
RRID:AB_312691
This monoclonal targets CD4
View all literature mentionsFITC anti-mouse/human CD44 (antibody)
RRID:AB_493684
This monoclonal targets CD44
View all literature mentionsFITC anti-mouse CD45.2 (antibody)
RRID:AB_313442
This monoclonal targets CD45.2
View all literature mentions
