The CHD Protein Kismet Restricts the Synaptic Localization of Cell Adhesion Molecules at the Drosophila Neuromuscular Junction.
The appropriate expression and localization of cell surface cell adhesion molecules must be tightly regulated for optimal synaptic growth and function. How neuronal plasma membrane proteins, including cell adhesion molecules, cycle between early endosomes and the plasma membrane is poorly understood. Here we show that the Drosophila homolog of the chromatin remodeling enzymes CHD7 and CHD8, Kismet, represses the synaptic levels of several cell adhesion molecules. Neuroligins 1 and 3 and the integrins αPS2 and βPS are increased at kismet mutant synapses but Kismet only directly regulates transcription of neuroligin 2. Kismet may therefore regulate synaptic CAMs indirectly by activating transcription of gene products that promote intracellular vesicle trafficking including endophilin B (endoB) and/or rab11. Knock down of EndoB in all tissues or neurons increases synaptic FasII while knock down of EndoB in kis mutants does not produce an additive increase in FasII. In contrast, neuronal expression of Rab11, which is deficient in kis mutants, leads to a further increase in synaptic FasII in kis mutants. These data support the hypothesis that Kis influences the synaptic localization of FasII by promoting intracellular vesicle trafficking through the early endosome.
Pubmed ID: 38474321
Research resources used in this publication
- w[*]; P{w[+mW.hs]=GawB}how[24B] (RRID:BDSC_1767) (organism)
- w[*]; P{w[+mC]=GAL4-elav.L}CG16779[3] (RRID:BDSC_8760) (organism)
- y[1] w[*]; P{w[+mC]=UASp-YFP.Rab11}3 (RRID:BDSC_50782) (organism)
- P{w[+mC]=UASp-YFP.Rab11.Q70L}20, y[1] w[*] (RRID:BDSC_50783) (organism)
- y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01285}attP2 (RRID:BDSC_34935) (organism)
- w[1118] (RRID:BDSC_3605) (organism)
- Bloomington Drosophila Stock Center (RRID:SCR_006457) (biomaterial supply resource)
- w[1118]; P{w[+mC]=GAL4-Act5C(FRT.CD2).P}S, P{w[+mC]=UAS-RFP.W}3/TM3, Sb[1] (RRID:BDSC_30558) (organism)
- w[*]; P{w[+mW.hs]=GawB}D42 (RRID:BDSC_8816) (organism)
- w[1118]; P{w[+m*]=GAL4}repo/TM3, Sb[1] (RRID:BDSC_7415) (organism)
- y[1] w[*]; P{w[+mC]=UASp-YFP.Rab11.S25N}06 (RRID:BDSC_23261) (organism)
- y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS02143}attP2 (RRID:BDSC_40895) (organism)
- y[1] w[67c23]; P{w[+mC]=lacW}kis[k13416]/CyO (RRID:BDSC_10442) (organism)
Additional research tools detected in this publication
None foundAntibodies used in this publication
None foundAssociated grants
- Agency: NINDS NIH HHS, United States
Id: R15 NS101608 - Agency: NINDS NIH HHS, United States
Id: 1R15NS101608-01A1, 2R15NS101608-02A1
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w[*]; P{w[+mW.hs]=GawB}how[24B] (organism)
RRID:BDSC_1767
Drosophila melanogaster with name w[*]; P{w[+mW.hs]=GawB}how[24B] from BDSC.
View all literature mentionsw[*]; P{w[+mC]=GAL4-elav.L}CG16779[3] (organism)
RRID:BDSC_8760
Drosophila melanogaster with name w[*]; P{w[+mC]=GAL4-elav.L}CG16779[3] from BDSC.
View all literature mentionsy[1] w[*]; P{w[+mC]=UASp-YFP.Rab11}3 (organism)
RRID:BDSC_50782
Drosophila melanogaster with name y[1] w[*]; P{w[+mC]=UASp-YFP.Rab11}3 from BDSC.
View all literature mentionsP{w[+mC]=UASp-YFP.Rab11.Q70L}20, y[1] w[*] (organism)
RRID:BDSC_50783
Drosophila melanogaster with name P{w[+mC]=UASp-YFP.Rab11.Q70L}20, y[1] w[*] from BDSC.
View all literature mentionsy[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01285}attP2 (organism)
RRID:BDSC_34935
Drosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01285}attP2 from BDSC.
View all literature mentionsw[1118] (organism)
RRID:BDSC_3605
Drosophila melanogaster with name w[1118] from BDSC.
View all literature mentionsBloomington Drosophila Stock Center (biomaterial supply resource)
RRID:SCR_006457
Collects, maintains and distributes Drosophila melanogaster strains for research. Emphasis is placed on genetic tools that are useful to a broad range of investigations. These include basic stocks of flies used in genetic analysis such as marker, balancer, mapping, and transposon-tagging strains; mutant alleles of identified genes, including a large set of transposable element insertion alleles; defined sets of deficiencies and a variety of other chromosomal aberrations; engineered lines for somatic and germline clonal analysis; GAL4 and UAS lines for targeted gene expression; enhancer trap and lacZ-reporter strains with defined expression patterns for marking tissues; and a collection of transposon-induced lethal mutations.
View all literature mentionsw[1118]; P{w[+mC]=GAL4-Act5C(FRT.CD2).P}S, P{w[+mC]=UAS-RFP.W}3/TM3, Sb[1] (organism)
RRID:BDSC_30558
Drosophila melanogaster with name w[1118]; P{w[+mC]=GAL4-Act5C(FRT.CD2).P}S, P{w[+mC]=UAS-RFP.W}3/TM3, Sb[1] from BDSC.
View all literature mentionsw[*]; P{w[+mW.hs]=GawB}D42 (organism)
RRID:BDSC_8816
Drosophila melanogaster with name w[*]; P{w[+mW.hs]=GawB}D42 from BDSC.
View all literature mentionsw[1118]; P{w[+m*]=GAL4}repo/TM3, Sb[1] (organism)
RRID:BDSC_7415
Drosophila melanogaster with name w[1118]; P{w[+m*]=GAL4}repo/TM3, Sb[1] from BDSC.
View all literature mentionsy[1] w[*]; P{w[+mC]=UASp-YFP.Rab11.S25N}06 (organism)
RRID:BDSC_23261
Drosophila melanogaster with name y[1] w[*]; P{w[+mC]=UASp-YFP.Rab11.S25N}06 from BDSC.
View all literature mentionsy[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS02143}attP2 (organism)
RRID:BDSC_40895
Drosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS02143}attP2 from BDSC.
View all literature mentionsy[1] w[67c23]; P{w[+mC]=lacW}kis[k13416]/CyO (organism)
RRID:BDSC_10442
Drosophila melanogaster with name y[1] w[67c23]; P{w[+mC]=lacW}kis[k13416]/CyO from BDSC.
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