Neonatal mouse hearts have transient renewal capacity, which is lost in juvenile and adult stages. In neonatal mouse hearts, myocardial infarction (MI) causes an initial loss of cardiomyocytes. However, it is unclear which type of regulated cell death (RCD) occurs in stressed cardiomyocytes. In the current studies, we induced MI in neonatal and juvenile mouse hearts and showed that ischemic cardiomyocytes primarily undergo ferroptosis, a non-apoptotic and iron-dependent form of RCD. We demonstrated that cardiac fibroblasts (CFs) protect cardiomyocytes from ferroptosis through paracrine effects and direct cell-cell interaction. CFs show strong resistance to ferroptosis due to high ferritin expression. The fibrogenic activity of CFs, typically considered detrimental to heart function, is negatively regulated by paired-like homeodomain 2 (Pitx2) signaling from cardiomyocytes. In addition, Pitx2 prevents ferroptosis in cardiomyocytes by regulating ferroptotic genes. Understanding the regulatory mechanisms of cardiomyocyte survival and death can identify potentially translatable therapeutic strategies for MI.
Pubmed ID: 38469561 RIS Download
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View all literature mentionsThis polyclonal secondary targets IgG (H+L)
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View all literature mentionsThis monoclonal targets CD181 (CXCR1)
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View all literature mentionsThis polyclonal targets 4 Hydroxynonenal
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View all literature mentionsThis recombinant monoclonal targets Ferritin Heavy Chain
View all literature mentionsThis monoclonal targets Rabbit n/a
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Pitx2ABC
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Donkey Goat IgG (H+L) Alexa Fluor® 633
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets AMID
View all literature mentionsThis unknown targets ACSL4
View all literature mentionsThis monoclonal targets Cardiac Troponin T
View all literature mentionsThis unknown targets CXCR2
View all literature mentionsThis monoclonal targets CD182
View all literature mentionsThis monoclonal targets CD181 (CXCR1)
View all literature mentionsThis monoclonal targets α-tubulin
View all literature mentionsThis monoclonal targets ferritin heavy chain (B-12)
View all literature mentionsThis polyclonal targets Thrombospondin antibody
View all literature mentionsThis monoclonal targets Vimentin antibody [EPR3776]
View all literature mentionsThis polyclonal targets Mouse PDGF R alpha
View all literature mentionsThis polyclonal targets Connexin-43
View all literature mentionsThis polyclonal targets Connexin 45/ GJA7/ Cx45
View all literature mentionsThis monoclonal targets Ferritin Light Chain antibody [EPR5260]
View all literature mentionsThis polyclonal targets TGF beta Receptor II
View all literature mentionsThis monoclonal targets phospho-smad2
View all literature mentionsThis recombinant monoclonal targets Smad3 (phospho S423 + S425)
View all literature mentionsThis monoclonal targets Actin alpha-Smooth Muscle - Cy3(TM) antibody produced in mouse
View all literature mentionsThis monoclonal targets Troponin T, Cardiac Isoform
View all literature mentionsThis polyclonal targets COX2/ Cyclooxygenase 2/ PTGS2
View all literature mentionsThis monoclonal targets MLKL
View all literature mentionsThis polyclonal targets 4 Hydroxynonenal
View all literature mentionsThis monoclonal targets Casp3
View all literature mentionsThis monoclonal targets myosin, sarcomere (MHC)
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