Unraveling the interplay between PKA inhibition and Cdk1 activation during oocyte meiotic maturation.
Oocytes are arrested in prophase I. In vertebrates, meiotic resumption is triggered by hormonal stimulation that results in cAMP-dependent protein kinase (PKA) downregulation leading to Cdk1 activation. Yet the pathways connecting PKA to Cdk1 remain unclear. Here, we identify molecular events triggered by PKA downregulation occurring upstream of Cdk1 activation. We describe a two-step regulation controlling cyclin B1 and Mos accumulation, which depends on both translation and stabilization. Cyclin B1 accumulation is triggered by PKA inhibition upstream of Cdk1 activation, while its translation requires Cdk1 activity. Conversely, Mos translation initiates in response to the hormone, but the protein accumulates only downstream of Cdk1. Furthermore, two successive translation waves take place, the first controlled by PKA inhibition and the second by Cdk1 activation. Notably, Arpp19, an essential PKA effector, does not regulate the early PKA-dependent events. This study elucidates how PKA downregulation orchestrates multiple pathways that converge toward Cdk1 activation and induce the oocyte G2/M transition.
Pubmed ID: 38358892 RIS Download
Research resources used in this publication
None foundAdditional research tools detected in this publication
None foundAntibodies used in this publication
- Rabbit Anti-Glutathione-S-Transferase (GST) Antibody, Horseradish Peroxidase Conjugated (RRID:AB_258340)
- CDC2 [3E1.1] antibody (RRID:AB_423957)
- ERK 2 (C-14) (RRID:AB_2141292)
- Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (RRID:AB_331768)
- Rabbit Anti-cdc25C, phospho (Ser216) Monoclonal Antibody, Unconjugated, Clone 63F9 (RRID:AB_331215)
- PLK1 (phospho T210) antibody [2A3] (RRID:AB_10861033)
- Anti-Puromycin Antibody, clone 12D10 (RRID:AB_2566826)
- Phospho-cdc2 (Tyr15) Antibody (RRID:AB_331460)
- Rabbit Anti-PPP1A, phospho (Thr320) Monoclonal Antibody, Unconjugated, Clone EP1512Y (RRID:AB_956236)
- ERK 1 (C-16) (RRID:AB_631453)
- Mosxe (C237) (RRID:AB_673780)
- Mouse Anti-Cyclin B2 Monoclonal Antibody, Unconjugated, Clone X29.2 (RRID:AB_444357)
- karyopherin beta1 (C-19) (RRID:AB_2134005)
Associated grants
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This is a list of tools and resources that we have found mentioned in this publication.
Rabbit Anti-Glutathione-S-Transferase (GST) Antibody, Horseradish Peroxidase Conjugated (antibody)
RRID:AB_258340
This unknown targets Glutathione-S-Transferase (GST)
View all literature mentionsCDC2 [3E1.1] antibody (antibody)
RRID:AB_423957
This monoclonal targets CDC2 [3E1.1] antibody
View all literature mentionsPhospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (antibody)
RRID:AB_331768
This monoclonal targets Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb
View all literature mentionsRabbit Anti-cdc25C, phospho (Ser216) Monoclonal Antibody, Unconjugated, Clone 63F9 (antibody)
RRID:AB_331215
This monoclonal targets cdc25C, phospho (Ser216)
View all literature mentionsPLK1 (phospho T210) antibody [2A3] (antibody)
RRID:AB_10861033
This monoclonal targets PLK1 (phospho T210) antibody [2A3]
View all literature mentionsAnti-Puromycin Antibody, clone 12D10 (antibody)
RRID:AB_2566826
This monoclonal targets Puromycin from Streptomyces alboniger
View all literature mentionsPhospho-cdc2 (Tyr15) Antibody (antibody)
RRID:AB_331460
This unknown targets cdc2, phospho (Tyr15)
View all literature mentionsRabbit Anti-PPP1A, phospho (Thr320) Monoclonal Antibody, Unconjugated, Clone EP1512Y (antibody)
RRID:AB_956236
This monoclonal targets PPP1A (phospho T320)
View all literature mentionsERK 1 (C-16) (antibody)
RRID:AB_631453
This polyclonal targets ERK 1 (C-16)
View all literature mentionsMosxe (C237) (antibody)
RRID:AB_673780
This polyclonal targets Mosxe (C237)
View all literature mentionsMouse Anti-Cyclin B2 Monoclonal Antibody, Unconjugated, Clone X29.2 (antibody)
RRID:AB_444357
This monoclonal targets Cyclin B2
View all literature mentionskaryopherin beta1 (C-19) (antibody)
RRID:AB_2134005
This polyclonal targets KPNB1
View all literature mentions
