Embryonic temporal-spatial delineation of excitatory spinal V3 interneuron diversity.
Spinal neural circuits that execute movement are composed of cardinal classes of neurons that emerged from distinct progenitor lineages. Each cardinal class contains multiple neuronal subtypes characterized by distinct molecular, anatomical, and physiological characteristics. Through a focus on the excitatory V3 interneuron class, here we demonstrate that interneuron subtype diversity is delineated through a combination of neurogenesis timing and final laminar settling position. We have revealed that early-born and late-born embryonic V3 temporal classes further diversify into subclasses with spatially and molecularly discrete identities. While neurogenesis timing accounts for V3 morphological diversification, laminar settling position accounts for electrophysiological profiles distinguishing V3 subtypes within the same temporal classes. Furthermore, V3 interneuron subtypes display independent behavioral recruitment patterns demonstrating a functional modularity underlying V3 interneuron diversity. These studies provide a framework for how early embryonic temporal and spatial mechanisms combine to delineate spinal interneuron classes into molecularly, anatomically, and functionally relevant subtypes in adults.
Pubmed ID: 38160393 RIS Download
Research resources used in this publication
- Imaris (RRID:SCR_007370) (software resource)
- ZEN-ZEISS Efficient Navigation (RRID:SCR_021725) (image processing software)
- ImageJ (RRID:SCR_003070) (software resource)
- B6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J (RRID:IMSR_JAX:021875) (organism)
- B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (RRID:IMSR_JAX:007914) (organism)
- GraphPad Prism (RRID:SCR_002798) (software resource)
- MATLAB (RRID:SCR_001622) (software resource)
- pClamp (RRID:SCR_011323) (software resource)
Additional research tools detected in this publication
None foundAntibodies used in this publication
- anti-tdTomato (RRID:AB_2722750)
- Rabbit Anti-Prox1 , Unconjugated (RRID:AB_177485)
- Lucifer Yellow Polyclonal Antibody, Biotin (RRID:AB_2536191)
- Nkx2.2 transcription factor antibody - Jessell, T.M. / Brenner-Morton, S.; HHMI/Columbia University (RRID:AB_531794)
- Mouse ROBO3 Affinity Purified Polyclonal Ab (RRID:AB_10644167)
- RFP antibody [5F8] (RRID:AB_2336064)
- Living Colors® DsRed Polyclonal Antibody (RRID:AB_10013483)
- Goat Anti-SP8 Polyclonal Antibody, Unconjugated (RRID:AB_2194626)
- FOXP2 (N-16) (RRID:AB_2107124)
- NR5A2 (C-17) (RRID:AB_2154063)
- MAFB-human (RRID:AB_1079293)
- Nurr1 (M-196) (RRID:AB_2267355)
- Goat Anti-LMO3 Polyclonal Antibody, Unconjugated (RRID:AB_2136576)
- Human ONECUT2/OC-2 Affinity Purified Polyclonal Ab (RRID:AB_10640365)
- HNF-6 (H-100) (RRID:AB_2251852)
- BETA 3 (E-17) (RRID:AB_2065343)
- PROX1 antibody [5G10] (RRID:AB_10563321)
- Recombinant Anti-Oct-2 antibody [EPR12482] (RRID:AB_2889931)
- Mouse Anti-Human ERR gamma Monoclonal Antibody, Unconjugated, Clone H6812 (RRID:AB_1964234)
- Cy5-AffiniPure Donkey Anti-Sheep IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot) (RRID:AB_2340730)
- Cy5-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) (min X Bov,Ck,Gt,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot) (RRID:AB_2340462)
- Alexa Fluor 647-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot) (RRID:AB_2492288)
- Alexa Fluor 647-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Rat,Shp Sr Prot) (RRID:AB_2340863)
- Alexa Fluor 594 AffiniPure Donkey Anti-Goat IgG(H+L) (RRID:AB_2340433)
- Alexa Fluor 594-AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Shp Sr Prot) (RRID:AB_2340689)
- Alexa Fluor® 594 AffiniPure Donkey Anti-Rabbit IgG (H+L) (RRID:AB_2340621)
- Alexa Fluor 488-AffiniPure Donkey Anti-Rabbit IgG (H+L) (RRID:AB_2313584)
- Donkey Anti-Goat IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (RRID:AB_2534102)
- Alexa Fluor® 488 AffiniPure F(ab')₂ Fragment Donkey Anti-Guinea Pig IgG (H+L) (RRID:AB_2617153)
- Alexa Fluor 488-AffiniPure Donkey Anti-Sheep IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot) (RRID:AB_2340745)
- Alexa Fluor 488-AffiniPure Goat Anti-Rat IgG (H+L) (RRID:AB_2338351)
- Alexa Fluor 488-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot) (RRID:AB_2340846)
Associated grants
- Agency: NINDS NIH HHS, United States
Id: R01 NS123116
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This is a list of tools and resources that we have found mentioned in this publication.
anti-tdTomato (antibody)
RRID:AB_2722750
This polyclonal targets tdTomato
View all literature mentionsRabbit Anti-Prox1 , Unconjugated (antibody)
RRID:AB_177485
This unknown targets Prox1
View all literature mentionsLucifer Yellow Polyclonal Antibody, Biotin (antibody)
RRID:AB_2536191
This unknown targets Lucifer Yellow
View all literature mentionsNkx2.2 transcription factor antibody - Jessell, T.M. / Brenner-Morton, S.; HHMI/Columbia University (antibody)
RRID:AB_531794
This monoclonal targets Nkx2.2 transcription factor
View all literature mentionsMouse ROBO3 Affinity Purified Polyclonal Ab (antibody)
RRID:AB_10644167
This polyclonal targets Mouse ROBO3 Affinity Purified Ab
View all literature mentionsRFP antibody [5F8] (antibody)
RRID:AB_2336064
This monoclonal targets dsRed, mRFP, mCherry , mPlum, mRFPruby
View all literature mentionsLiving Colors® DsRed Polyclonal Antibody (antibody)
RRID:AB_10013483
This polyclonal targets DsRed
View all literature mentionsGoat Anti-SP8 Polyclonal Antibody, Unconjugated (antibody)
RRID:AB_2194626
This polyclonal targets Sp8
View all literature mentionsNurr1 (M-196) (antibody)
RRID:AB_2267355
This polyclonal targets Nurr1 (M-196)
View all literature mentionsGoat Anti-LMO3 Polyclonal Antibody, Unconjugated (antibody)
RRID:AB_2136576
This polyclonal targets LMO3
View all literature mentionsHuman ONECUT2/OC-2 Affinity Purified Polyclonal Ab (antibody)
RRID:AB_10640365
This polyclonal targets Human ONECUT2/OC-2 Affinity Purified Ab
View all literature mentionsHNF-6 (H-100) (antibody)
RRID:AB_2251852
This polyclonal targets HNF-6 (H-100)
View all literature mentionsBETA 3 (E-17) (antibody)
RRID:AB_2065343
This polyclonal targets BHLHE22
View all literature mentionsPROX1 antibody [5G10] (antibody)
RRID:AB_10563321
This monoclonal targets PROX1 antibody [5G10]
View all literature mentionsRecombinant Anti-Oct-2 antibody [EPR12482] (antibody)
RRID:AB_2889931
This recombinant monoclonal targets [Oct-2]
View all literature mentionsMouse Anti-Human ERR gamma Monoclonal Antibody, Unconjugated, Clone H6812 (antibody)
RRID:AB_1964234
This monoclonal targets Human ERR gamma
View all literature mentionsCy5-AffiniPure Donkey Anti-Sheep IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot) (antibody)
RRID:AB_2340730
This unknown targets Sheep IgG (H+L)
View all literature mentionsCy5-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) (min X Bov,Ck,Gt,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot) (antibody)
RRID:AB_2340462
This unknown targets Guinea Pig IgG (H+L)
View all literature mentionsAlexa Fluor 647-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot) (antibody)
RRID:AB_2492288
This polyclonal targets Rabbit IgG
View all literature mentionsAlexa Fluor 647-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Rat,Shp Sr Prot) (antibody)
RRID:AB_2340863
This unknown targets Mouse IgG (H+L)
View all literature mentionsAlexa Fluor 594 AffiniPure Donkey Anti-Goat IgG(H+L) (antibody)
RRID:AB_2340433
This polyclonal secondary targets IgG (H+L)
View all literature mentionsAlexa Fluor 594-AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Shp Sr Prot) (antibody)
RRID:AB_2340689
This unknown targets Rat IgG (H+L)
View all literature mentionsAlexa Fluor® 594 AffiniPure Donkey Anti-Rabbit IgG (H+L) (antibody)
RRID:AB_2340621
This polyclonal targets IgG (H+L)
View all literature mentionsAlexa Fluor 488-AffiniPure Donkey Anti-Rabbit IgG (H+L) (antibody)
RRID:AB_2313584
This polyclonal secondary targets IgG (H+L)
View all literature mentionsDonkey Anti-Goat IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (antibody)
RRID:AB_2534102
This unknown targets IgG (H+L)
View all literature mentionsAlexa Fluor® 488 AffiniPure F(ab')₂ Fragment Donkey Anti-Guinea Pig IgG (H+L) (antibody)
RRID:AB_2617153
This polyclonal targets Guinea Pig
View all literature mentionsAlexa Fluor 488-AffiniPure Donkey Anti-Sheep IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot) (antibody)
RRID:AB_2340745
This unknown targets Sheep IgG (H+L)
View all literature mentionsAlexa Fluor 488-AffiniPure Goat Anti-Rat IgG (H+L) (antibody)
RRID:AB_2338351
This unknown targets Rat IgG (H+L)
View all literature mentionsAlexa Fluor 488-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot) (antibody)
RRID:AB_2340846
This unknown targets Mouse IgG (H+L)
View all literature mentionsImaris (software resource)
RRID:SCR_007370
Imaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.
View all literature mentionsZEN-ZEISS Efficient Navigation (image processing software)
RRID:SCR_021725
Software package for ZEISS light microscopy systems. Universal user interface for every imaging system from ZEISS to assist to acquire images, process images, visualize big data by GPU powered 3D engine, analyze images via Machine Learning-based tools, correlate between light-light or light-electron microscopes, store raw data in secure format locally or in the cloud.
View all literature mentionsImageJ (software resource)
RRID:SCR_003070
Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsB6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J (organism)
RRID:IMSR_JAX:021875
Mus musculus with name B6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J from IMSR.
View all literature mentionsB6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (organism)
RRID:IMSR_JAX:007914
Mus musculus with name B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J from IMSR.
View all literature mentionsGraphPad Prism (software resource)
RRID:SCR_002798
Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsMATLAB (software resource)
RRID:SCR_001622
Multi paradigm numerical computing environment and fourth generation programming language developed by MathWorks. Allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces, and interfacing with programs written in other languages, including C, C++, Java, Fortran and Python. Used to explore and visualize ideas and collaborate across disciplines including signal and image processing, communications, control systems, and computational finance.
View all literature mentionspClamp (software resource)
RRID:SCR_011323
Software suite for electrophysiology data acquisition and analysis by Molecular Devices. Used for the control and recording of voltage clamp, current clamp, and patch clamp experiments. The software suite consists of Clampex 11 Software for data acquisition, AxoScope 11 Software for background recording, Clampfit 11 Software for data analysis, and optional Clampfit Advanced Analysis Module for sophisticated and streamlined analysis.
View all literature mentions
