Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.
Aerobic glycolysis is a pivotal hallmark of cancers, including colorectal cancer. Evidence shows glycolytic enzymes are regulated by post-translational modifications (PTMs), thereby affecting the Warburg effect and reprograming cancer metabolism. Lysine lactylation is a PTM reported in 2019 in histones. In this study, we identified protein lactylation in FHC cells and SW480 colon cancer cells through mass spectrometry. Totally, 637 lysine lactylation sites in 444 proteins were identified in FHC and SW480 cells. Lactylated proteins were enriched in the glycolysis pathway, and we identified lactylation sites in phosphofructokinase, platelet (PFKP) lysine 688 and aldolase A (ALDOA) lysine 147. We also showed that PFKP lactylation directly attenuated enzyme activity. Collectively, our study presented a resource to investigate proteome-wide lactylation in SW480 cells and found PFKP lactylation led to activity inhibition, indicating that lactic acid and lactylated PFKP may form a negative feedback pathway in glycolysis and lactic acid production.
Pubmed ID: 38155775 RIS Download
Research resources used in this publication
None foundAdditional research tools detected in this publication
- ATCC (RRID:SCR_001672)
- R Project for Statistical Computing (RRID:SCR_001905)
- UniProt (RRID:SCR_002380)
- GraphPad Prism (RRID:SCR_002798)
- ImageJ (RRID:SCR_003070)
- PRISM (RRID:SCR_005375)
- Promega (RRID:SCR_006724)
- Thermo Fisher Scientific (RRID:SCR_008452)
- Millipore (RRID:SCR_008983)
- KEGG (RRID:SCR_012773)
- MaxQuant (RRID:SCR_014485)
- GeneSys (RRID:SCR_015770)
- clusterProfiler (RRID:SCR_016884)
Antibodies used in this publication
- IPKine™ HRP, Mouse Anti-Rabbit IgG LCS (RRID:AB_2893334)
- HRP* Goat Anti Mouse IgG (H+L) (RRID:AB_2943495)
- Alpha Tubulin antibody (RRID:AB_11042766)
- GAPDH antibody (RRID:AB_2263076)
- PFKP antibody (RRID:AB_2252278)
- ALDOA antibody (RRID:AB_2224626)
- Beta Actin antibody (RRID:AB_10700003)
- Anti-L-Lactyl-Histone H4 (Lys12) Rabbit mAb (RRID:AB_2941896)
- Anti-L-lactyllysine rabbit pAb (RRID:AB_2868521)
Associated grants
NonePublication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This is a list of tools and resources that we have found mentioned in this publication.
ATCC (tool)
RRID:SCR_001672
Global nonprofit biological resource center (BRC) and research organization that provides biological products, technical services and educational programs to private industry, government and academic organizations. Its mission is to acquire, authenticate, preserve, develop and distribute biological materials, information, technology, intellectual property and standards for the advancement and application of scientific knowledge. The primary purpose of ATCC is to use its resources and experience as a BRC to become the world leader in standard biological reference materials management, intellectual property resource management and translational research as applied to biomaterial development, standardization and certification. ATCC characterizes cell lines, bacteria, viruses, fungi and protozoa, as well as develops and evaluates assays and techniques for validating research resources and preserving and distributing biological materials to the public and private sector research communities.
View all literature mentionsR Project for Statistical Computing (tool)
RRID:SCR_001905
Software environment and programming language for statistical computing and graphics. R is integrated suite of software facilities for data manipulation, calculation and graphical display. Can be extended via packages. Some packages are supplied with the R distribution and more are available through CRAN family.It compiles and runs on wide variety of UNIX platforms, Windows and MacOS.
View all literature mentionsUniProt (tool)
RRID:SCR_002380
Collection of data of protein sequence and functional information. Resource for protein sequence and annotation data. Consortium for preservation of the UniProt databases: UniProt Knowledgebase (UniProtKB), UniProt Reference Clusters (UniRef), and UniProt Archive (UniParc), UniProt Proteomes. Collaboration between European Bioinformatics Institute (EMBL-EBI), SIB Swiss Institute of Bioinformatics and Protein Information Resource. Swiss-Prot is a curated subset of UniProtKB.
View all literature mentionsGraphPad Prism (tool)
RRID:SCR_002798
Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsImageJ (tool)
RRID:SCR_003070
Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsPRISM (tool)
RRID:SCR_005375
THIS RESOURCE IS NO LONGER IN SERVICE. Documented on May 5,2022.Tool that predicts interactions between transcription factors and their regulated genes from binding motifs. Understanding vertebrate development requires unraveling the cis-regulatory architecture of gene regulation. PRISM provides accurate genome-wide computational predictions of transcription factor binding sites for the human and mouse genomes, and integrates the predictions with GREAT to provide functional biological context. Together, accurate computational binding site prediction and GREAT produce for each transcription factor: 1. putative binding sites, 2. putative target genes, 3. putative biological roles of the transcription factor, and 4. putative cis-regulatory elements through which the factor regulates each target in each functional role.
View all literature mentionsThermo Fisher Scientific (tool)
RRID:SCR_008452
Commercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.
View all literature mentionsKEGG (tool)
RRID:SCR_012773
Integrated database resource consisting of 16 main databases, broadly categorized into systems information, genomic information, and chemical information. In particular, gene catalogs in completely sequenced genomes are linked to higher-level systemic functions of cell, organism, and ecosystem. Analysis tools are also available. KEGG may be used as reference knowledge base for biological interpretation of large-scale datasets generated by sequencing and other high-throughput experimental technologies.
View all literature mentionsMaxQuant (tool)
RRID:SCR_014485
A quantitative proteomics software package for analyzing large-scale mass-spectrometric data sets. It is a set of algorithms that include peak detection and scoring of peptides, mass calibration, database searches for protein identification, protein quantification, and provides summary statistics.
View all literature mentionsGeneSys (tool)
RRID:SCR_015770
Imaging software that works with G:BOX Chemi and PXi imaging systems to automatically or manually capture images. GeneSys also includes a database containing information about applications such as fluorescence, chemiluminescence and chemifluorescence.
View all literature mentionsclusterProfiler (tool)
RRID:SCR_016884
Software R package for statistical analysis and visualization of functional profiles for genes and gene clusters.
View all literature mentionsIPKine™ HRP, Mouse Anti-Rabbit IgG LCS (antibody)
RRID:AB_2893334
This monoclonal targets IgG LCS
View all literature mentionsHRP* Goat Anti Mouse IgG (H+L) (antibody)
RRID:AB_2943495
This polyclonal secondary targets IgG (H+L)
View all literature mentionsAlpha Tubulin antibody (antibody)
RRID:AB_11042766
This monoclonal targets Alpha Tubulin
View all literature mentionsGAPDH antibody (antibody)
RRID:AB_2263076
This polyclonal targets GAPDH
View all literature mentionsALDOA antibody (antibody)
RRID:AB_2224626
This polyclonal targets ALDOA
View all literature mentionsBeta Actin antibody (antibody)
RRID:AB_10700003
This polyclonal targets Beta Actin
View all literature mentionsAnti-L-Lactyl-Histone H4 (Lys12) Rabbit mAb (antibody)
RRID:AB_2941896
This monoclonal targets L-Lactyl-Histone H4 (Lys12)
View all literature mentionsAnti-L-lactyllysine rabbit pAb (antibody)
RRID:AB_2868521
This polyclonal targets lactyllysine
View all literature mentions
