Generation and Characterization of a Human-Derived and Induced Pluripotent Stem Cell (iPSC) Line from an Alzheimer's Disease Patient with Neuropsychiatric Symptoms.
Agitation is one of the most eminent characteristics of neuropsychiatric symptoms (NPS) affecting people living with Alzheimer's and Dementia and has serious consequences for patients and caregivers. The current consensus is that agitation results, in part, from the disruption of ascending monoamine regulators of cortical circuits, especially the loss of serotonergic activity. It is believed that the first line of treatment for these conditions is selective serotonin reuptake inhibitors (SSRIs), but these are effective in only about 40% of patients. Person-specific biomarkers, for example, ones based on in vitro iPSC-derived models of serotonin activity, which predict who with Agitation responds to an SSRI, are a major clinical priority. Here, we report the generation of human-induced pluripotent stem cells (iPSCs) from a 74-year-old AD patient, the homozygous APOE ε4/ε4 carrier, who developed Agitation. His iPSCs were reprogrammed from peripheral blood mononuclear cells (PBMCs) using the transient expression of pluripotency genes. These display typical iPSC characteristics that are karyotypically normal and attain the capacity to differentiate into three germ layers. The newly patient-derived iPSC line offers a unique resource to investigate the underlying mechanisms associated with neuropsychiatric symptom progression in AD.
Pubmed ID: 38137534 RIS Download
Research resources used in this publication
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None foundAntibodies used in this publication
- Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555 (RRID:AB_2535849)
- Rabbit anti-MTUS1 Antibody, Affinity Purified (RRID:AB_1907294)
- Anti-CD11a / LFA-1a Monoclonal Antibody, FITC Conjugated (RRID:AB_2128995)
- MAP2 (D5G1) XP® (RRID:AB_2722660)
- Anti-Actin, smooth muscle, clone ASM-1 (RRID:AB_2223166)
- Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2576217)
- Neuronal Class III beta-Tubulin (TUJ1) Monoclonal Antibody, Purified (RRID:AB_2313773)
- AFP Monoclonal Antibody (P5B8) (RRID:AB_10987005)
- Mouse Anti-Human Nanog Monoclonal Antibody, V450 Conjugated (RRID:AB_1645598)
- POU5F1-human (RRID:AB_2167703)
- Anti-TRA-1-60, clone TRA-1-60 (RRID:AB_2119183)
Associated grants
- Agency: NIA NIH HHS, United States
Id: R01 AG052510 - Agency: NIA NIH HHS, United States
Id: R21 AG067016 - Agency: NIA NIH HHS, United States
Id: R21AG067016 - Agency: NIH HHS, United States
Id: R01AG052510
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This is a list of tools and resources that we have found mentioned in this publication.
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555 (antibody)
RRID:AB_2535849
This polyclonal secondary targets IgG (H+L)
View all literature mentionsRabbit anti-MTUS1 Antibody, Affinity Purified (antibody)
RRID:AB_1907294
This polyclonal targets MTUS1
View all literature mentionsAnti-CD11a / LFA-1a Monoclonal Antibody, FITC Conjugated (antibody)
RRID:AB_2128995
This monoclonal targets CD11a / LFA-1a FITC
View all literature mentionsMAP2 (D5G1) XP® (antibody)
RRID:AB_2722660
This monoclonal targets MAP2
View all literature mentionsAnti-Actin, smooth muscle, clone ASM-1 (antibody)
RRID:AB_2223166
This monoclonal targets Acta2
View all literature mentionsGoat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_2576217
This polyclonal secondary targets IgG (H+L)
View all literature mentionsNeuronal Class III beta-Tubulin (TUJ1) Monoclonal Antibody, Purified (antibody)
RRID:AB_2313773
This monoclonal targets Neuronal Class III beta-Tubulin (TUJ1) Purified
View all literature mentionsAFP Monoclonal Antibody (P5B8) (antibody)
RRID:AB_10987005
This monoclonal targets AFP
View all literature mentionsMouse Anti-Human Nanog Monoclonal Antibody, V450 Conjugated (antibody)
RRID:AB_1645598
This monoclonal targets Human Nanog
View all literature mentionsAnti-TRA-1-60, clone TRA-1-60 (antibody)
RRID:AB_2119183
This monoclonal targets HSP90B1
View all literature mentions
