BACH1 changes microglial metabolism and affects astrogenesis during mouse brain development.
Microglia are highly heterogeneous as resident immune cells in the central nervous system. Although the proinflammatory phenotype of microglia is driven by the metabolic transformation in the disease state, the mechanism of metabolic reprogramming in microglia and whether it affects surrounding astrocyte progenitors have not been well elucidated. Here, we illustrate the communication between microglial metabolism and astrogenesis during embryonic development. The transcription factor BTB and CNC homology 1 (Bach1) reduces lactate production by inhibiting two key enzymes, HK2 and GAPDH, during glycolysis. Metabolic perturbation of microglia reduces lactate-dependent histone modification enrichment at the Lrrc15 promoter. The microglia-derived LRRC15 interacts with CD248 to participate in the JAK/STAT pathway and influence astrogenesis. In addition, Bach1cKO-Cx3 mice exhibit abnormal neuronal differentiation and anxiety-like behaviors. Altogether, this work suggests that the maintenance of microglia metabolic homeostasis during early brain development is closely related to astrogenesis, providing insights into astrogenesis and related diseases.
Pubmed ID: 38101413 RIS Download
Research resources used in this publication
Additional research tools detected in this publication
None foundAntibodies used in this publication
- CD11b Monoclonal Antibody (M1/70), APC, eBioscience (RRID:AB_469344)
- PE anti-mouse CD45 (RRID:AB_312970)
- Stat3 (79D7) Rabbit mAb (RRID:AB_331269)
- Phospho-Stat3 (Tyr705) (D3A7) XP Rabbit mAb (RRID:AB_2491009)
- Phospho-Jak1 (Tyr1034/1035)/Jak2 (Tyr1007/1008) (E9Y7V) Mouse mAb (RRID:AB_2799703)
- Lactyl-Histone H3 (Lys18) Rabbit mAb (RRID:AB_2909438)
- Anti-L-lactyllysine rabbit pAb (RRID:AB_2868521)
- Rabbit IgG Isotype Control, ALEXA FLUOR® 647 Conjugated (RRID:AB_3068583)
- HA-Tag (C29F4) Rabbit mAb (RRID:AB_1549585)
- Monoclonal ANTI-FLAG® M2 antibody produced in mouse (RRID:AB_262044)
- Beta Actin antibody (RRID:AB_10700003)
- Beta Actin antibody (RRID:AB_2289225)
- Mouse Anti-ALDH1L1 Monoclonal Antibody, Unconjugated (RRID:AB_940204)
- Rabbit Anti-S100 beta Monoclonal Antibody, Unconjugated, Clone EP1576Y (RRID:AB_882426)
- Glial Fibrillary Acidic Protein (Multipurpose) (RRID:AB_10013382)
- Rat Anti-Mouse CD68 Monoclonal antibody, Unconjugated, Clone FA-11 (RRID:AB_324217)
- P2Y12/P2RY12 Antibody (RRID:AB_2810254)
- Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (RRID:AB_2070042)
- CX3CR1 antibody (RRID:AB_306203)
- GLAST antibody (RRID:AB_2878738)
- BACH1 (F-9) (RRID:AB_10608972)
- BACH1 Polyclonal Antibody (RRID:AB_2766202)
- Rabbit Anti-BLBP Polyclonal Antibody, Unconjugated (RRID:AB_880078)
- Anti Iba1, Rabbit antibody (RRID:AB_839504)
- Iba1 antibody (RRID:AB_2224402)
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This is a list of tools and resources that we have found mentioned in this publication.
CD11b Monoclonal Antibody (M1/70), APC, eBioscience (antibody)
RRID:AB_469344
This monoclonal targets CD11b
View all literature mentionsPE anti-mouse CD45 (antibody)
RRID:AB_312970
This monoclonal targets CD45
View all literature mentionsStat3 (79D7) Rabbit mAb (antibody)
RRID:AB_331269
This recombinant monoclonal targets Stat3
View all literature mentionsPhospho-Stat3 (Tyr705) (D3A7) XP Rabbit mAb (antibody)
RRID:AB_2491009
This monoclonal targets Phospho-Stat3 (Tyr705)
View all literature mentionsPhospho-Jak1 (Tyr1034/1035)/Jak2 (Tyr1007/1008) (E9Y7V) Mouse mAb (antibody)
RRID:AB_2799703
This monoclonal targets JAK2
View all literature mentionsLactyl-Histone H3 (Lys18) Rabbit mAb (antibody)
RRID:AB_2909438
This recombinant monoclonal targets Lactyl-Histone H3 (Lys18)
View all literature mentionsAnti-L-lactyllysine rabbit pAb (antibody)
RRID:AB_2868521
This polyclonal targets lactyllysine
View all literature mentionsRabbit IgG Isotype Control, ALEXA FLUOR® 647 Conjugated (antibody)
RRID:AB_3068583
This polyclonal targets not applicable
View all literature mentionsHA-Tag (C29F4) Rabbit mAb (antibody)
RRID:AB_1549585
This monoclonal targets HA-Tag
View all literature mentionsMonoclonal ANTI-FLAG® M2 antibody produced in mouse (antibody)
RRID:AB_262044
This monoclonal targets FLAG
View all literature mentionsBeta Actin antibody (antibody)
RRID:AB_10700003
This polyclonal targets Beta Actin
View all literature mentionsBeta Actin antibody (antibody)
RRID:AB_2289225
This monoclonal targets Beta Actin
View all literature mentionsMouse Anti-ALDH1L1 Monoclonal Antibody, Unconjugated (antibody)
RRID:AB_940204
This monoclonal targets ALDH1L1
View all literature mentionsRabbit Anti-S100 beta Monoclonal Antibody, Unconjugated, Clone EP1576Y (antibody)
RRID:AB_882426
This monoclonal targets S100 beta
View all literature mentionsGlial Fibrillary Acidic Protein (Multipurpose) (antibody)
RRID:AB_10013382
This polyclonal targets GFAP
View all literature mentionsRat Anti-Mouse CD68 Monoclonal antibody, Unconjugated, Clone FA-11 (antibody)
RRID:AB_324217
This monoclonal targets Mouse CD68
View all literature mentionsP2Y12/P2RY12 Antibody (antibody)
RRID:AB_2810254
This polyclonal targets P2Y12/P2RY12
View all literature mentionsCleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (antibody)
RRID:AB_2070042
This monoclonal targets Casp3
View all literature mentionsCX3CR1 antibody (antibody)
RRID:AB_306203
This polyclonal targets CX3CR1
View all literature mentionsGLAST antibody (antibody)
RRID:AB_2878738
This polyclonal targets GLAST
View all literature mentionsBACH1 (F-9) (antibody)
RRID:AB_10608972
This monoclonal targets BACH1 (F-9)
View all literature mentionsBACH1 Polyclonal Antibody (antibody)
RRID:AB_2766202
This polyclonal targets BACH1
View all literature mentionsRabbit Anti-BLBP Polyclonal Antibody, Unconjugated (antibody)
RRID:AB_880078
This polyclonal targets BLBP - Neuronal Marker
View all literature mentionsAnti Iba1, Rabbit antibody (antibody)
RRID:AB_839504
This polyclonal targets Iba1
View all literature mentionsFVB-Tg(GFAP-cre)25Mes/J (organism)
RRID:IMSR_JAX:004600
Mus musculus with name FVB-Tg(GFAP-cre)25Mes/J from IMSR.
View all literature mentionsB6.129P2(Cg)-Cx3cr1tm1Litt/J (organism)
RRID:IMSR_JAX:005582
Mus musculus with name B6.129P2(Cg)-Cx3cr1tm1Litt/J from IMSR.
View all literature mentionsB6J.B6N(Cg)-Cx3cr1tm1.1(cre)Jung/J (organism)
RRID:IMSR_JAX:025524
Mus musculus with name B6J.B6N(Cg)-Cx3cr1tm1.1(cre)Jung/J from IMSR.
View all literature mentionsNeuro-2a (cell line)
RRID:CVCL_0470
Cell line Neuro-2a is a Cancer cell line with a species of origin Mus musculus
View all literature mentionsHEK293 (cell line)
RRID:CVCL_0045
Cell line HEK293 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentions
