Priming of macrophage by glycosphingolipids from extracellular vesicles facilitates immune tolerance for embryo-maternal crosstalk.
Glycosphingolipids (GSLs) display diverse functions during embryonic development. Here, we examined the GSL profiles of extracellular vesicles (EVs) secreted from human embryonic stem cells (hESCs) and investigated their functions in priming macrophages to enhance immune tolerance of embryo implantation. When peripheral blood mononuclear cells were incubated with ESC-secreted EVs, globo-series GSLs (GHCer, SSEA3Cer, and SSEA4Cer) were transferred via EVs into monocytes/macrophages. Incubation of monocytes during their differentiation into macrophages with either EVs or synthetic globo-series GSLs induced macrophages to exhibit phenotypic features that imitate immune receptivity, i.e., macrophage polarization, augmented phagocytic activity, suppression of T cell proliferation, and the increased trophoblast invasion. It was also demonstrated that decidual macrophages in first-trimester tissues expressed globo-series GSLs. These findings highlight the role of globo-series GSLs via transfer from EVs in priming macrophages to display decidual macrophage phenotypes, which may facilitate healthy pregnancy.
Pubmed ID: 37989081 RIS Download
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- Goat anti-Rat IgM (Heavy chain) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2535798)
- Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_141607)
- Alkaline Phosphatase-AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot) (RRID:AB_2337953)
- Alkaline Phosphatase-AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Sw Sr Prot) (RRID:AB_2338538)
- 4nm Colloidal Gold-AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Sw Sr Prot) (LM Grade) (RRID:AB_2338723)
- Purified anti-human CD28 (RRID:AB_314303)
- LEAF™ Purified anti-human CD3 (RRID:AB_571925)
- Rat Anti-Mouse Trem-2b Monoclonal antibody, Unconjugated, Clone 237920 (RRID:AB_2208679)
- CD209 Rabbit mAb (RRID:AB_2863747)
- Mouse Anti-SSEA-4 Monoclonal antibody, Unconjugated, Clone mc-813-70 (RRID:AB_357704)
- Rat Anti-SSEA-3 Monoclonal antibody, Unconjugated, Clone mc-631 (RRID:AB_357703)
- PE anti-human CD4 (RRID:AB_2562036)
- Brilliant Violet 421(TM) anti-human CD14 (RRID:AB_2563296)
- Anti-β-Actin Antibody (RRID:AB_476744)
- Anti-CD9 Antibody with goat anti-rabbit HRP secondary antibody (RRID:AB_2687469)
- CD63 antibody [MEM-259] (RRID:AB_306364)
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This is a list of tools and resources that we have found mentioned in this publication.
Goat anti-Rat IgM (Heavy chain) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_2535798
This polyclonal secondary targets IgM (Heavy chain)
View all literature mentionsDonkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_141607
This polyclonal secondary targets IgG (H+L)
View all literature mentionsAlkaline Phosphatase-AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot) (antibody)
RRID:AB_2337953
This unknown targets Rabbit IgG (H+L)
View all literature mentionsAlkaline Phosphatase-AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Sw Sr Prot) (antibody)
RRID:AB_2338538
This unknown targets Mouse IgG (H+L)
View all literature mentions4nm Colloidal Gold-AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Sw Sr Prot) (LM Grade) (antibody)
RRID:AB_2338723
This unknown targets Mouse IgG (H+L)
View all literature mentionsPurified anti-human CD28 (antibody)
RRID:AB_314303
This monoclonal targets CD28
View all literature mentionsLEAF™ Purified anti-human CD3 (antibody)
RRID:AB_571925
This monoclonal targets CD3
View all literature mentionsRat Anti-Mouse Trem-2b Monoclonal antibody, Unconjugated, Clone 237920 (antibody)
RRID:AB_2208679
This monoclonal targets Mouse TREM-2b
View all literature mentionsMouse Anti-SSEA-4 Monoclonal antibody, Unconjugated, Clone mc-813-70 (antibody)
RRID:AB_357704
This monoclonal targets SSEA-4
View all literature mentionsRat Anti-SSEA-3 Monoclonal antibody, Unconjugated, Clone mc-631 (antibody)
RRID:AB_357703
This monoclonal targets SSEA-3
View all literature mentionsPE anti-human CD4 (antibody)
RRID:AB_2562036
This monoclonal targets CD4
View all literature mentionsBrilliant Violet 421(TM) anti-human CD14 (antibody)
RRID:AB_2563296
This monoclonal targets CD14
View all literature mentionsAnti-β-Actin Antibody (antibody)
RRID:AB_476744
This monoclonal targets slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH
View all literature mentionsAnti-CD9 Antibody with goat anti-rabbit HRP secondary antibody (antibody)
RRID:AB_2687469
This unknown targets CD9
View all literature mentionsCD63 antibody [MEM-259] (antibody)
RRID:AB_306364
This monoclonal targets CD63
View all literature mentionsImageJ (software resource)
RRID:SCR_003070
Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsGraphPad Prism (software resource)
RRID:SCR_002798
Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsLeica Application Suite X (resource)
RRID:SCR_013673
Software for image capture, processing and analysis with Leica fluorescence and confocal microscopes.
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RRID:SCR_008520
Software for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsHUES 6 (cell line)
RRID:CVCL_B194
Cell line HUES 6 is a Embryonic stem cell with a species of origin Homo sapiens (Human)
View all literature mentionsWA09 (cell line)
RRID:CVCL_9773
Cell line WA09 is a Embryonic stem cell with a species of origin Homo sapiens (Human)
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