Peptide-mediated inhibition of the transcriptional regulator Elongin BC induces apoptosis in cancer cells.
Inhibition of protein-protein interactions (PPIs) via designed peptides is an effective strategy to perturb their biological functions. The Elongin BC heterodimer (ELOB/C) binds to a BC-box motif and is essential for cancer cell growth. Here, we report a peptide that mimics the high-affinity BC-box of the PRC2-associated protein EPOP. This peptide tightly binds to the ELOB/C dimer (kD = 0.46 ± 0.02 nM) and blocks the association of ELOB/C with its interaction partners, both in vitro and in the cellular environment. Cancer cells treated with our peptide inhibitor showed decreased cell viability, increased apoptosis, and perturbed gene expression. Therefore, our work proposes that blocking the BC-box-binding pocket of ELOB/C is a feasible strategy to impair its function and inhibit cancer cell growth. Our peptide inhibitor promises novel mechanistic insights into the biological function of the ELOB/C dimer and offers a starting point for therapeutics linked to ELOB/C dysfunction.
Pubmed ID: 37354906 RIS Download
Research resources used in this publication
Additional research tools detected in this publication
None foundAntibodies used in this publication
- HA (hemagglutinen protein) (RRID:AB_2314621)
- Goat Anti-Rat IgG (H+L) Antibody, Alexa Fluor ?? 546 Conjugated (RRID:AB_141738)
- Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2535794)
- Goat Anti-Rat IgG, Whole Ab ECL Antibody, HRP Conjugated (RRID:AB_772207)
- Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (RRID:AB_143165)
- Donkey Anti-Rabbit IgG, Whole Ab ECL Antibody, HRP Conjugated (RRID:AB_772206)
- Sheep Anti-Mouse IgG - Horseradish Peroxidase (RRID:AB_772210)
- GST (B-14) (RRID:AB_627677)
- Mouse Anti-Tubulin, beta Monoclonal antibody, Unconjugated, Clone kmx-1 (RRID:AB_94650)
- SUZ12-human (RRID:AB_2614929)
- H3-celegans (RRID:AB_302613)
- Monoclonal ANTI-FLAG® M2 antibody (RRID:AB_259529)
- TCEB1 Antibody (RRID:AB_2620982)
- Anti-TCEB2 / Elongin-B antibody (RRID:AB_2783826)
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This is a list of tools and resources that we have found mentioned in this publication.
HA (hemagglutinen protein) (antibody)
RRID:AB_2314621
This unknown targets
View all literature mentionsGoat Anti-Rat IgG (H+L) Antibody, Alexa Fluor ?? 546 Conjugated (antibody)
RRID:AB_141738
This unknown targets Rat IgG (H+L)
View all literature mentionsDonkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_2535794
This polyclonal secondary targets IgG (H+L)
View all literature mentionsGoat Anti-Rat IgG, Whole Ab ECL Antibody, HRP Conjugated (antibody)
RRID:AB_772207
This polyclonal secondary targets IgG
View all literature mentionsGoat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (antibody)
RRID:AB_143165
This polyclonal targets IgG (H+L)
View all literature mentionsDonkey Anti-Rabbit IgG, Whole Ab ECL Antibody, HRP Conjugated (antibody)
RRID:AB_772206
This unknown targets IgG
View all literature mentionsSheep Anti-Mouse IgG - Horseradish Peroxidase (antibody)
RRID:AB_772210
This monoclonal secondary targets IgG
View all literature mentionsGST (B-14) (antibody)
RRID:AB_627677
This monoclonal targets GST (B-14)
View all literature mentionsMouse Anti-Tubulin, beta Monoclonal antibody, Unconjugated, Clone kmx-1 (antibody)
RRID:AB_94650
This monoclonal targets Tubulin, beta
View all literature mentionsMonoclonal ANTI-FLAG® M2 antibody (antibody)
RRID:AB_259529
This monoclonal targets FLAG
View all literature mentionsAnti-TCEB2 / Elongin-B antibody (antibody)
RRID:AB_2783826
This polyclonal targets TCEB2 / Elongin-B
View all literature mentionsMIA PaCa-2 (cell line)
RRID:CVCL_0428
Cell line MIA PaCa-2 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsHEK293 (cell line)
RRID:CVCL_0045
Cell line HEK293 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsHEK293T (cell line)
RRID:CVCL_0063
Cell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsNCI-H23 (cell line)
RRID:CVCL_1547
Cell line NCI-H23 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsHep-G2 (cell line)
RRID:CVCL_0027
Cell line Hep-G2 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsSH-SY5Y (cell line)
RRID:CVCL_0019
Cell line SH-SY5Y is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsMCF-7 (cell line)
RRID:CVCL_0031
Cell line MCF-7 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsPC-3 (cell line)
RRID:CVCL_0035
Cell line PC-3 is a Cancer cell line with a species of origin Homo sapiens (Human)
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