LBP1C-2 from Lycium barbarum maintains skeletal muscle satellite cell pool by interaction with FGFR1.
Muscle stem cells, called satellite cells (SCs), are employed to repair and rebuild muscle. SC-based therapeutic strategies are urgently needed. In this study, we showed that Lycium barbarum extract (LBE) improved the number of SCs and enhanced muscle regeneration by promoting SC activation and self-renewal in both adult and aging mice. L. barbarum polysaccharide (LBP), the main component of LBE, also played a similar role. More importantly, LBP1C-2, a homogeneous polysaccharide isolated from LBP, was uncovered to be an active component in regulating SC function. Mechanism study revealed that LBP1C-2 might bind to FGFR1 to activate SCs and promote SC self-renewal through Spry1 upregulation. This might be the first study to show that LBE participated in the regulation of SCs, and the active components and targets of LBE were identified. This study lays a theoretical foundation for the medicinal or auxiliary medicinal use of L. barbarum in skeletal muscle.
Pubmed ID: 37138777 RIS Download
Research resources used in this publication
None foundAdditional research tools detected in this publication
Antibodies used in this publication
- p38 MAPK (D13E1) XP Rabbit mAb (RRID:AB_10999090)
- Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP(tm) Rabbit mAb (RRID:AB_2139682)
- FGFR1 antibody [EPR806Y] (RRID:AB_1523613)
- FGFR1 (phospho Y653 + Y654) antibody (RRID:AB_10859625)
- Myosin 4 Monoclonal Antibody (MF20), eBioscience (RRID:AB_2572894)
- Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, FITC (RRID:AB_2536525)
- MyoD (C-20) (RRID:AB_631992)
- Cy3-Streptavidin antibody (RRID:AB_2337244)
- Biotin-SP-AffiniPure Goat Anti-Mouse IgG, Fc_ Subclass 1 Specific (min X Hu,Bov,Rb Sr Prot) (RRID:AB_2338571)
- Pax7 antibody - Kawakami, A.; Tokyo Institute of Technology (RRID:AB_528428)
- Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE-Cyanine7, eBioscience (RRID:AB_469668)
- CD45 Monoclonal Antibody (30-F11), APC, eBioscience (RRID:AB_469392)
- CD31 (PECAM-1) Monoclonal Antibody (390), PE, eBioscience (RRID:AB_465632)
- CD34 Monoclonal Antibody (RAM34), FITC, eBioscience (RRID:AB_465020)
Associated grants
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This is a list of tools and resources that we have found mentioned in this publication.
C2C12 (tool)
RRID:CVCL_0188
Cell line C2C12 is a Spontaneously immortalized cell line with a species of origin Mus musculus (Mouse)
View all literature mentionsp38 MAPK (D13E1) XP Rabbit mAb (antibody)
RRID:AB_10999090
This monoclonal targets p38 MAPK (D13E1) XP Rabbit mAb
View all literature mentionsPhospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP(tm) Rabbit mAb (antibody)
RRID:AB_2139682
This monoclonal targets Mapk14
View all literature mentionsFGFR1 antibody [EPR806Y] (antibody)
RRID:AB_1523613
This monoclonal targets Human FGFR1
View all literature mentionsFGFR1 (phospho Y653 + Y654) antibody (antibody)
RRID:AB_10859625
This monoclonal targets FGFR1 (phospho Y653 + Y654) antibody
View all literature mentionsMyosin 4 Monoclonal Antibody (MF20), eBioscience (antibody)
RRID:AB_2572894
This monoclonal targets Myosin 4
View all literature mentionsGoat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, FITC (antibody)
RRID:AB_2536525
This polyclonal secondary targets IgG (H+L)
View all literature mentionsMyoD (C-20) (antibody)
RRID:AB_631992
This polyclonal targets MyoD (C-20)
View all literature mentionsCy3-Streptavidin antibody (antibody)
RRID:AB_2337244
This polyclonal targets Cy3-Streptavidin
View all literature mentionsBiotin-SP-AffiniPure Goat Anti-Mouse IgG, Fc_ Subclass 1 Specific (min X Hu,Bov,Rb Sr Prot) (antibody)
RRID:AB_2338571
This unknown targets Mouse IgG, Fc? Subclass 1 Specific
View all literature mentionsPax7 antibody - Kawakami, A.; Tokyo Institute of Technology (antibody)
RRID:AB_528428
This monoclonal targets Pax7
View all literature mentionsLy-6A/E (Sca-1) Monoclonal Antibody (D7), PE-Cyanine7, eBioscience (antibody)
RRID:AB_469668
This monoclonal targets Ly-6A/E (Sca-1)
View all literature mentionsCD45 Monoclonal Antibody (30-F11), APC, eBioscience (antibody)
RRID:AB_469392
This monoclonal targets CD45
View all literature mentionsCD31 (PECAM-1) Monoclonal Antibody (390), PE, eBioscience (antibody)
RRID:AB_465632
This monoclonal targets CD31 (PECAM-1)
View all literature mentionsCD34 Monoclonal Antibody (RAM34), FITC, eBioscience (antibody)
RRID:AB_465020
This monoclonal targets CD34
View all literature mentions
