Niche-expressed Galectin-1 is involved in pre-B acute lymphoblastic leukemia relapse through pre-B cell receptor activation.
B-cell acute lymphoblastic leukemia (B-ALL) reflects the malignant counterpart of developing B cells in the bone marrow (BM). Despite tremendous progress in B-ALL treatment, the overall survival of adults at diagnosis and patients at all ages after relapse remains poor. Galectin-1 (GAL1) expressed by BM supportive niches delivers proliferation signals to normal pre-B cells through interaction with the pre-B cell receptor (pre-BCR). Here, we asked whether GAL1 gives non-cell autonomous signals to pre-BCR+ pre-B ALL, in addition to cell-autonomous signals linked to genetic alterations. In syngeneic and patient-derived xenograft (PDX) murine models, murine and human pre-B ALL development is influenced by GAL1 produced by BM niches through pre-BCR-dependent signals, similarly to normal pre-B cells. Furthermore, targeting pre-BCR signaling together with cell-autonomous oncogenic pathways in pre-B ALL PDX improved treatment response. Our results show that non-cell autonomous signals transmitted by BM niches represent promising targets to improve B-ALL patient survival.
Pubmed ID: 37009219 RIS Download
Research resources used in this publication
Additional research tools detected in this publication
None foundAntibodies used in this publication
- Cytofix/Cytoperm Soln Kit (RRID:AB_2869008)
- Streptavidin-BD Horizon(tm) PE-CF594 (RRID:AB_11154598)
- Alexa Fluor 647-AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (RRID:AB_2338921)
- Alexa Fluor 488-AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Shp Sr Prot) (RRID:AB_2340684)
- BLNK (pY84) (RRID:AB_647182)
- Syk (pY348) (RRID:AB_647247)
- Goat Anti-Human Galectin-1 Polyclonal antibody, Unconjugated (RRID:AB_2136626)
- Goat anti-Human IgM (Heavy chain) Secondary Antibody (RRID:AB_2535614)
- Mouse Anti-IgM Monoclonal Antibody, Phycoerythrin Conjugated, Clone G20-127 (RRID:AB_396118)
- Rat Anti-Integrin beta 7 Monoclonal Antibody, Allophycocyanin Conjugated, Clone FIB504 (RRID:AB_398490)
- CD49e (Integrin α5 chain) (RRID:AB_2739840)
- Mouse Anti-CD49d Monoclonal Antibody, Phycoerythrin Conjugated, Clone 9F10 (RRID:AB_395893)
- CD19 (RRID:AB_11154408)
- CD45 (RRID:AB_2744404)
- Anti-Human CD45 (LCA; leucocyte common antigen; T200) Monoclonal Antibody, FITC Conjugated, Clone J.33 (RRID:AB_131000)
- Pacific Blue(TM) anti-human CD45 (RRID:AB_493655)
- Mouse Anti-Human CD19 Monoclonal Antibody, PE-Cy7 Conjugated, Clone HIB19 (RRID:AB_1727438)
- Goat Anti-Mouse IgM-AF488 (RRID:AB_2794251)
- IgM (RRID:AB_2737671)
- Ig, λ1, λ2, & λ3 Light Chain (RRID:AB_2742297)
- Ig, κ light chain (RRID:AB_2737867)
- Mouse Anti-CD10 Monoclonal Antibody, PE-Cy5 (BD Cy-Chrome???) Conjugated, Clone HI10a (RRID:AB_395777)
- Ms Ly-6A/E BUV737 E13-161.7 50ug (RRID:AB_2873577)
- Rat Anti-Mouse Galectin-1 Monoclonal antibody, Unconjugated, Clone 201002 (RRID:AB_2136636)
- Endomucin (V.7C7) (RRID:AB_2100037)
- Rat Anti-Integrin beta 7 chain Monoclonal Antibody, Phycoerythrin Conjugated, Clone M293 (RRID:AB_396735)
- Rat Anti-Ly-51 Monoclonal Antibody, Biotin Conjugated, Clone 6C3 (RRID:AB_394671)
- Goat Anti-Mouse IgM-PE (RRID:AB_2794244)
- CD71 (Transferrin Receptor) (RRID:AB_2737950)
- Ms CD51 BV650 RMV-7 50ug (RRID:AB_2870640)
- CD49e (Integrin alpha 5) Monoclonal Antibody (eBioHMa5-1 (HMa5-1)), FITC, eBioscience (RRID:AB_1234968)
- CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), PE-Cyanine7, eBioscience (RRID:AB_2811776)
- Rat Anti-Mouse CD45 Antibody, Alexa Fluor??700 Conjugated (RRID:AB_1645208)
- CD45.1 Monoclonal Antibody (A20), APC, eBioscience (RRID:AB_469398)
- CD45R (B220) Monoclonal Antibody (RA3-6B2), APC-eFluor™ 780, eBioscience (RRID:AB_1518810)
- CD45 Monoclonal Antibody (30-F11), APC-eFluor™ 780, eBioscience (RRID:AB_1548781)
- CD45 Monoclonal Antibody (30-F11), PE-Cyanine5.5, eBioscience (RRID:AB_469718)
- BUV395 Rat Anti-Mouse CD45 Clone 30-F11 (RUO) (RRID:AB_2651134)
- Rat Anti-Mouse CD44 Monoclonal Antibody, APC-Cy7 Conjugated, Clone IM7 (RRID:AB_1727481)
- CD19 Monoclonal Antibody (eBio1D3 (1D3)), PE-Cyanine5.5, eBioscience (RRID:AB_891395)
- PE-CF594 Rat Anti-Mouse CD4 Clone RM4-5 (RRID:AB_11154410)
- CD8a (RRID:AB_394081)
- PE/Cyanine7 anti-mouse CD2 (RRID:AB_2563092)
- CD31 (PECAM-1) (RRID:AB_2739979)
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This is a list of tools and resources that we have found mentioned in this publication.
Cytofix/Cytoperm Soln Kit (antibody)
RRID:AB_2869008
This unknown targets
View all literature mentionsStreptavidin-BD Horizon(tm) PE-CF594 (antibody)
RRID:AB_11154598
This unknown targets Streptavidin-BD Horizon(tm) PE-CF594
View all literature mentionsAlexa Fluor 647-AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (antibody)
RRID:AB_2338921
This unknown targets Mouse IgG (H+L)
View all literature mentionsAlexa Fluor 488-AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Shp Sr Prot) (antibody)
RRID:AB_2340684
This unknown targets Rat IgG (H+L)
View all literature mentionsBLNK (pY84) (antibody)
RRID:AB_647182
This unknown targets BLNK, phospho (Tyr84)
View all literature mentionsSyk (pY348) (antibody)
RRID:AB_647247
This monoclonal targets Syk (pY348)
View all literature mentionsGoat Anti-Human Galectin-1 Polyclonal antibody, Unconjugated (antibody)
RRID:AB_2136626
This polyclonal targets Human Galectin-1
View all literature mentionsGoat anti-Human IgM (Heavy chain) Secondary Antibody (antibody)
RRID:AB_2535614
This polyclonal secondary targets IgM (Heavy chain)
View all literature mentionsMouse Anti-IgM Monoclonal Antibody, Phycoerythrin Conjugated, Clone G20-127 (antibody)
RRID:AB_396118
This monoclonal targets IgM
View all literature mentionsRat Anti-Integrin beta 7 Monoclonal Antibody, Allophycocyanin Conjugated, Clone FIB504 (antibody)
RRID:AB_398490
This monoclonal targets Integrin β7 chain
View all literature mentionsCD49e (Integrin α5 chain) (antibody)
RRID:AB_2739840
This monoclonal targets CD49e (Integrin α5 chain)
View all literature mentionsMouse Anti-CD49d Monoclonal Antibody, Phycoerythrin Conjugated, Clone 9F10 (antibody)
RRID:AB_395893
This monoclonal targets CD49d (Integrin α4 chain)
View all literature mentionsAnti-Human CD45 (LCA; leucocyte common antigen; T200) Monoclonal Antibody, FITC Conjugated, Clone J.33 (antibody)
RRID:AB_131000
This monoclonal targets Human CD45 (LCA; leucocyte common antigen; T200)
View all literature mentionsPacific Blue(TM) anti-human CD45 (antibody)
RRID:AB_493655
This monoclonal targets CD45
View all literature mentionsMouse Anti-Human CD19 Monoclonal Antibody, PE-Cy7 Conjugated, Clone HIB19 (antibody)
RRID:AB_1727438
This monoclonal targets Human CD19
View all literature mentionsGoat Anti-Mouse IgM-AF488 (antibody)
RRID:AB_2794251
This unknown targets IgM
View all literature mentionsIg, λ1, λ2, & λ3 Light Chain (antibody)
RRID:AB_2742297
This monoclonal targets Ig, λ1, λ2, & λ3 Light Chain
View all literature mentionsIg, κ light chain (antibody)
RRID:AB_2737867
This monoclonal targets Ig, κ light chain
View all literature mentionsMouse Anti-CD10 Monoclonal Antibody, PE-Cy5 (BD Cy-Chrome???) Conjugated, Clone HI10a (antibody)
RRID:AB_395777
This monoclonal targets CD10
View all literature mentionsMs Ly-6A/E BUV737 E13-161.7 50ug (antibody)
RRID:AB_2873577
This monoclonal targets Ly-6A/E
View all literature mentionsRat Anti-Mouse Galectin-1 Monoclonal antibody, Unconjugated, Clone 201002 (antibody)
RRID:AB_2136636
This monoclonal targets Mouse Galectin-1
View all literature mentionsEndomucin (V.7C7) (antibody)
RRID:AB_2100037
This monoclonal targets Mouse Endomucin
View all literature mentionsRat Anti-Integrin beta 7 chain Monoclonal Antibody, Phycoerythrin Conjugated, Clone M293 (antibody)
RRID:AB_396735
This monoclonal targets Integrin beta 7 chain
View all literature mentionsRat Anti-Ly-51 Monoclonal Antibody, Biotin Conjugated, Clone 6C3 (antibody)
RRID:AB_394671
This monoclonal targets Ly-51
View all literature mentionsGoat Anti-Mouse IgM-PE (antibody)
RRID:AB_2794244
This unknown targets IgM
View all literature mentionsCD71 (Transferrin Receptor) (antibody)
RRID:AB_2737950
This monoclonal targets CD71 (Transferrin Receptor)
View all literature mentionsMs CD51 BV650 RMV-7 50ug (antibody)
RRID:AB_2870640
This monoclonal targets CD51 (Integrin αV chain)
View all literature mentionsCD49e (Integrin alpha 5) Monoclonal Antibody (eBioHMa5-1 (HMa5-1)), FITC, eBioscience (antibody)
RRID:AB_1234968
This monoclonal targets CD49e (Integrin alpha 5)
View all literature mentionsCD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), PE-Cyanine7, eBioscience (antibody)
RRID:AB_2811776
This monoclonal targets CD49d (Integrin alpha 4)
View all literature mentionsRat Anti-Mouse CD45 Antibody, Alexa Fluor??700 Conjugated (antibody)
RRID:AB_1645208
This unknown targets Mouse CD45
View all literature mentionsCD45.1 Monoclonal Antibody (A20), APC, eBioscience (antibody)
RRID:AB_469398
This monoclonal targets CD45.1
View all literature mentionsCD45R (B220) Monoclonal Antibody (RA3-6B2), APC-eFluor™ 780, eBioscience (antibody)
RRID:AB_1518810
This monoclonal targets CD45R (B220)
View all literature mentionsCD45 Monoclonal Antibody (30-F11), APC-eFluor™ 780, eBioscience (antibody)
RRID:AB_1548781
This monoclonal targets CD45
View all literature mentionsCD45 Monoclonal Antibody (30-F11), PE-Cyanine5.5, eBioscience (antibody)
RRID:AB_469718
This monoclonal targets CD45
View all literature mentionsBUV395 Rat Anti-Mouse CD45 Clone 30-F11 (RUO) (antibody)
RRID:AB_2651134
This monoclonal targets CD45
View all literature mentionsRat Anti-Mouse CD44 Monoclonal Antibody, APC-Cy7 Conjugated, Clone IM7 (antibody)
RRID:AB_1727481
This monoclonal targets Mouse CD44
View all literature mentionsCD19 Monoclonal Antibody (eBio1D3 (1D3)), PE-Cyanine5.5, eBioscience (antibody)
RRID:AB_891395
This monoclonal targets CD19
View all literature mentionsPE-CF594 Rat Anti-Mouse CD4 Clone RM4-5 (antibody)
RRID:AB_11154410
This monoclonal targets CD4
View all literature mentionsPE/Cyanine7 anti-mouse CD2 (antibody)
RRID:AB_2563092
This monoclonal targets CD2
View all literature mentionsCD31 (PECAM-1) (antibody)
RRID:AB_2739979
This monoclonal targets CD31 (PECAM-1)
View all literature mentionsNALM-6 (cell line)
RRID:CVCL_0092
Cell line NALM-6 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentions
