Tissue-resident Lachnospiraceae family bacteria protect against colorectal carcinogenesis by promoting tumor immune surveillance.
The intestinal microbiota plays an important role in colorectal cancer (CRC) progression. However, the effect of tissue-resident commensal bacteria on CRC immune surveillance remains poorly understood. Here, we analyzed the intratissue bacteria from CRC patient colon tissues. We found that the commensal bacteria belonging to the Lachnospiraceae family, including Ruminococcus gnavus (Rg), Blautia producta (Bp), and Dorea formicigenerans (Df), were enriched in normal tissues, while Fusobacterium nucleatum (Fn) and Peptostreptococcus anaerobius (Pa) were abundant in tumor tissues. Tissue-resident Rg and Bp reduced colon tumor growth and promoted the activation of CD8+ T cells in immunocompetent mice. Mechanistically, intratissue Rg and Bp degraded lyso-glycerophospholipids that inhibited CD8+ T cell activity and maintained the immune surveillance function of CD8+ T cells. Lyso-glycerophospholipids alone promoted tumor growth that was abrogated with Rg and Bp injection. Collectively, intratissue Lachnospiraceae family bacteria facilitate the immune surveillance function of CD8+ T cells and control colorectal cancer progression.
Pubmed ID: 36893736
Research resources used in this publication
- CT26.WT (RRID:CVCL_7256) (cell line)
- FITC anti-human CD16 (RRID:AB_2876771) (antibody)
- PE/Cyanine7 anti-mouse IFN-gamma (RRID:AB_2295770) (antibody)
- Brilliant Violet 605(TM) anti-mouse Ki-67 (RRID:AB_2562664) (antibody)
- PE/Cyanine7 anti-mouse CD45.2 (RRID:AB_1186103) (antibody)
- Brilliant Violet 510(TM) anti-mouse CD8a (RRID:AB_2561389) (antibody)
- ImageJ (RRID:SCR_003070) (software resource)
Additional research tools detected in this publication
None foundAntibodies used in this publication
- FITC anti-human CD16 (RRID:AB_2876771)
- PE/Cyanine7 anti-mouse IFN-gamma (RRID:AB_2295770)
- Brilliant Violet 605(TM) anti-mouse Ki-67 (RRID:AB_2562664)
- PE/Cyanine7 anti-mouse CD45.2 (RRID:AB_1186103)
- Brilliant Violet 510(TM) anti-mouse CD8a (RRID:AB_2561389)
- InVivoMab anti-mouse CD28 (RRID:AB_1107624)
- InVivoPlus anti-mouse CD3ε (RRID:AB_1107634)
- InVivoPlus rat IgG1 isotype control (RRID:AB_2687813)
- anti-mouse CD8α (RRID:AB_1125541)
- CD294 (CRTH2) Monoclonal Antibody (BM16), APC, eBioscience (RRID:AB_2573192)
- CD127 Monoclonal Antibody (eBioSB/199 (SB/199)), PerCP-eFluor™ 710, eBioscience (RRID:AB_2573710)
- KLRG1 Monoclonal Antibody (2F1), APC, eBioscience (RRID:AB_469469)
- CD335 (NKp46) Monoclonal Antibody (29A1.4), PE, eBioscience (RRID:AB_1210743)
- CD19 Monoclonal Antibody (eBio1D3 (1D3)), eFluor™ 450, eBioscience (RRID:AB_2734905)
- PerCP/Cyanine5.5 anti-human CD326 (Ep-CAM) (RRID:AB_893474)
- PE/Cyanine7 anti-human CD161 (RRID:AB_11126167)
- PE anti-human CD336 (NKp44) (RRID:AB_756100)
- PE anti-human CD117 (c-kit) (RRID:AB_2890809)
- Brilliant Violet 421(TM) anti-human CD127 (IL-7Ralpha) (RRID:AB_10898326)
- APC anti-human CD56 (NCAM) (RRID:AB_604098)
- APC anti-human CD14 (RRID:AB_830680)
- APC anti-mouse CD103 (RRID:AB_1227503)
- PerCP/Cyanine5.5 anti-human CD4 (RRID:AB_893328)
- PE/Cyanine7 anti-human CD8a (RRID:AB_314130)
- Brilliant Violet 510(TM) anti-human CD19 (RRID:AB_2564228)
- APC/Cyanine7 anti-human CD45 (RRID:AB_2566375)
- CD3 Monoclonal Antibody (17A2), eFluor™ 450, eBioscience (RRID:AB_1272193)
- T-bet Monoclonal Antibody (eBio4B10 (4B10)), PE, eBioscience (RRID:AB_925762)
- APC/Cyanine7 anti-mouse F4/80 (RRID:AB_893489)
- PerCP anti-mouse Ly-6G/Ly-6C (Gr-1) (RRID:AB_893560)
- APC anti-mouse/human CD11b (RRID:AB_312795)
- FITC anti-mouse CD11c (RRID:AB_313775)
- PE anti-mouse CD4 (RRID:AB_312692)
- PE anti-human/mouse Granzyme B Recombinant (RRID:AB_2687031)
- FITC anti-mouse TNF-alpha (RRID:AB_315424)
Associated grants
NonePublication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This is a list of tools and resources that we have found mentioned in this publication.
FITC anti-human CD16 (antibody)
RRID:AB_2876771
This monoclonal targets CD16
View all literature mentionsPE/Cyanine7 anti-mouse IFN-gamma (antibody)
RRID:AB_2295770
This monoclonal targets IFN-gamma
View all literature mentionsBrilliant Violet 605(TM) anti-mouse Ki-67 (antibody)
RRID:AB_2562664
This monoclonal targets Ki-67
View all literature mentionsPE/Cyanine7 anti-mouse CD45.2 (antibody)
RRID:AB_1186103
This monoclonal targets CD45.2
View all literature mentionsBrilliant Violet 510(TM) anti-mouse CD8a (antibody)
RRID:AB_2561389
This monoclonal targets CD8a
View all literature mentionsInVivoMab anti-mouse CD28 (antibody)
RRID:AB_1107624
This monoclonal targets CD28
View all literature mentionsInVivoPlus anti-mouse CD3ε (antibody)
RRID:AB_1107634
This monoclonal targets CD3ε
View all literature mentionsInVivoPlus rat IgG1 isotype control (antibody)
RRID:AB_2687813
This isotype control targets Trinitrophenol
View all literature mentionsanti-mouse CD8α (antibody)
RRID:AB_1125541
This monoclonal targets CD8α
View all literature mentionsCD294 (CRTH2) Monoclonal Antibody (BM16), APC, eBioscience (antibody)
RRID:AB_2573192
This monoclonal targets CD294 (CRTH2)
View all literature mentionsCD127 Monoclonal Antibody (eBioSB/199 (SB/199)), PerCP-eFluor™ 710, eBioscience (antibody)
RRID:AB_2573710
This monoclonal targets CD127
View all literature mentionsKLRG1 Monoclonal Antibody (2F1), APC, eBioscience (antibody)
RRID:AB_469469
This monoclonal targets KLRG1
View all literature mentionsCD335 (NKp46) Monoclonal Antibody (29A1.4), PE, eBioscience (antibody)
RRID:AB_1210743
This monoclonal targets CD335 (NKp46)
View all literature mentionsCD19 Monoclonal Antibody (eBio1D3 (1D3)), eFluor™ 450, eBioscience (antibody)
RRID:AB_2734905
This monoclonal targets CD19
View all literature mentionsPerCP/Cyanine5.5 anti-human CD326 (Ep-CAM) (antibody)
RRID:AB_893474
This monoclonal targets CD326
View all literature mentionsPE/Cyanine7 anti-human CD161 (antibody)
RRID:AB_11126167
This monoclonal targets CD161
View all literature mentionsPE anti-human CD336 (NKp44) (antibody)
RRID:AB_756100
This monoclonal targets CD336
View all literature mentionsPE anti-human CD117 (c-kit) (antibody)
RRID:AB_2890809
This monoclonal targets CD117
View all literature mentionsBrilliant Violet 421(TM) anti-human CD127 (IL-7Ralpha) (antibody)
RRID:AB_10898326
This monoclonal targets CD127
View all literature mentionsAPC anti-human CD56 (NCAM) (antibody)
RRID:AB_604098
This monoclonal targets CD56
View all literature mentionsAPC anti-human CD14 (antibody)
RRID:AB_830680
This monoclonal targets CD14
View all literature mentionsAPC anti-mouse CD103 (antibody)
RRID:AB_1227503
This monoclonal targets CD103
View all literature mentionsPerCP/Cyanine5.5 anti-human CD4 (antibody)
RRID:AB_893328
This monoclonal targets CD4
View all literature mentionsPE/Cyanine7 anti-human CD8a (antibody)
RRID:AB_314130
This monoclonal targets CD8a
View all literature mentionsBrilliant Violet 510(TM) anti-human CD19 (antibody)
RRID:AB_2564228
This monoclonal targets CD19
View all literature mentionsAPC/Cyanine7 anti-human CD45 (antibody)
RRID:AB_2566375
This monoclonal targets CD45
View all literature mentionsCD3 Monoclonal Antibody (17A2), eFluor™ 450, eBioscience (antibody)
RRID:AB_1272193
This monoclonal targets CD3
View all literature mentionsT-bet Monoclonal Antibody (eBio4B10 (4B10)), PE, eBioscience (antibody)
RRID:AB_925762
This monoclonal targets T-bet
View all literature mentionsAPC/Cyanine7 anti-mouse F4/80 (antibody)
RRID:AB_893489
This monoclonal targets F4/80
View all literature mentionsPerCP anti-mouse Ly-6G/Ly-6C (Gr-1) (antibody)
RRID:AB_893560
This monoclonal targets Ly-6G Ly-6C
View all literature mentionsAPC anti-mouse/human CD11b (antibody)
RRID:AB_312795
This monoclonal targets CD11b
View all literature mentionsFITC anti-mouse CD11c (antibody)
RRID:AB_313775
This monoclonal targets CD11c
View all literature mentionsPE anti-mouse CD4 (antibody)
RRID:AB_312692
This monoclonal targets CD4
View all literature mentionsPE anti-human/mouse Granzyme B Recombinant (antibody)
RRID:AB_2687031
This recombinant monoclonal targets Granzyme B
View all literature mentionsFITC anti-mouse TNF-alpha (antibody)
RRID:AB_315424
This monoclonal targets TNF-alpha
View all literature mentionsCT26.WT (cell line)
RRID:CVCL_7256
Cell line CT26.WT is a Cancer cell line with a species of origin Mus musculus
View all literature mentionsFITC anti-human CD16 (antibody)
RRID:AB_2876771
This monoclonal targets CD16
View all literature mentionsPE/Cyanine7 anti-mouse IFN-gamma (antibody)
RRID:AB_2295770
This monoclonal targets IFN-gamma
View all literature mentionsBrilliant Violet 605(TM) anti-mouse Ki-67 (antibody)
RRID:AB_2562664
This monoclonal targets Ki-67
View all literature mentionsPE/Cyanine7 anti-mouse CD45.2 (antibody)
RRID:AB_1186103
This monoclonal targets CD45.2
View all literature mentionsBrilliant Violet 510(TM) anti-mouse CD8a (antibody)
RRID:AB_2561389
This monoclonal targets CD8a
View all literature mentionsImageJ (software resource)
RRID:SCR_003070
Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentions
