A systems immunology study comparing innate and adaptive immune responses in adults to COVID-19 mRNA and adenovirus vectored vaccines.
Identifying the molecular mechanisms that promote optimal immune responses to coronavirus disease 2019 (COVID-19) vaccination is critical for future rational vaccine design. Here, we longitudinally profile innate and adaptive immune responses in 102 adults after the first, second, and third doses of mRNA or adenovirus-vectored COVID-19 vaccines. Using a multi-omics approach, we identify key differences in the immune responses induced by ChAdOx1-S and BNT162b2 that correlate with antigen-specific antibody and T cell responses or vaccine reactogenicity. Unexpectedly, we observe that vaccination with ChAdOx1-S, but not BNT162b2, induces an adenoviral vector-specific memory response after the first dose, which correlates with the expression of proteins with roles in thrombosis with potential implications for thrombosis with thrombocytopenia syndrome (TTS), a rare but serious adverse event linked to adenovirus-vectored vaccines. The COVID-19 Vaccine Immune Responses Study thus represents a major resource that can be used to understand the immunogenicity and reactogenicity of these COVID-19 vaccines.
Pubmed ID: 36871558 RIS Download
Research resources used in this publication
None foundAdditional research tools detected in this publication
None foundAntibodies used in this publication
- Streptavidin-HRP ELISA 1mL (RRID:AB_2868972)
- PE/Dazzle(TM) 594 anti-human Siglec-8 (RRID:AB_2629717)
- Mouse Anti-Human Interferon-gamma, Monoclonal Antibody, Biotinylated, clone 7-B6-1 (RRID:AB_907273)
- Mouse Anti-Human Interferon-gamma, Monoclonal Antibody, Unconjugated, clone 1-D1K (RRID:AB_907283)
- APC-H7 Mouse Anti-Human HLA-DR (RRID:AB_10611876)
- Fixable Viability Stain 780 200ug (RRID:AB_2869673)
- Granzyme B (RRID:AB_2737618)
- Hu CCR7 (CD197) BV421 2-L1-A (RRID:AB_2869843)
- Hu CD279 (PD-1) BUV737 EH12.1 100Tst (RRID:AB_2870118)
- Mouse Anti-Human CD197 Monoclonal Antibody, Alexa Fluor 647 Conjugated, Clone 150503 (RRID:AB_2033948)
- CD185 (CXCR5) Antibody, anti-human, PE-Vio® 770, REAfinity™ (RRID:AB_2733204)
- APC anti-human CD137 (4-1BB) (RRID:AB_830672)
- PE/Cyanine7 anti-human CD134 (OX40) (RRID:AB_10901161)
- CD123 (IL-3 Receptor α) (RRID:AB_2738038)
- CD86 (RRID:AB_10562395)
- CD69 (RRID:AB_2737680)
- Hu CD56 BUV563 NCAM16.2 100Tst (RRID:AB_2870213)
- FITC anti-human CD45RA (RRID:AB_314410)
- Hu CD45 BUV395 HI30 100Tst (RRID:AB_2869519)
- CD38 (RRID:AB_2738097)
- CD27 (RRID:AB_2738042)
- Brilliant Violet 785(TM) anti-human CD20 (RRID:AB_2566315)
- Mouse Anti-CD19 Monoclonal Antibody, Phycoerythrin Conjugated, Clone HIB19 (RRID:AB_395813)
- CD19 (RRID:AB_2744314)
- BV421 Mouse Anti-Human CD16 (RRID:AB_2716865)
- CD14 (RRID:AB_2737724)
- Mouse Anti-CD11c Monoclonal Antibody, Phycoerythrin Conjugated, Clone B-ly6 (RRID:AB_395793)
- CD8 (RRID:AB_2741262)
- BV650 Mouse Anti-Human CD8 (RRID:AB_2744462)
- CD4 (RRID:AB_2744420)
- CD4 (RRID:AB_2743943)
- CD3 (RRID:AB_2738603)
- Mouse Anti-CD3 Monoclonal Antibody, FITC Conjugated, Clone HIT3a (RRID:AB_395745)
- Goat anti-Human IgG (H+L) Secondary Antibody, HRP (RRID:AB_228269)
Associated grants
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This is a list of tools and resources that we have found mentioned in this publication.
Streptavidin-HRP ELISA 1mL (antibody)
RRID:AB_2868972
This unknown targets
View all literature mentionsPE/Dazzle(TM) 594 anti-human Siglec-8 (antibody)
RRID:AB_2629717
This monoclonal targets Siglec-8
View all literature mentionsMouse Anti-Human Interferon-gamma, Monoclonal Antibody, Biotinylated, clone 7-B6-1 (antibody)
RRID:AB_907273
This monoclonal targets Mouse Human Interferon-gamma ylated clone 7-B6-1
View all literature mentionsMouse Anti-Human Interferon-gamma, Monoclonal Antibody, Unconjugated, clone 1-D1K (antibody)
RRID:AB_907283
This monoclonal targets Mouse Human Interferon-gamma clone 1-D1K
View all literature mentionsAPC-H7 Mouse Anti-Human HLA-DR (antibody)
RRID:AB_10611876
This monoclonal targets HLA-DR
View all literature mentionsFixable Viability Stain 780 200ug (antibody)
RRID:AB_2869673
This unknown targets
View all literature mentionsGranzyme B (antibody)
RRID:AB_2737618
This monoclonal targets Granzyme B
View all literature mentionsHu CCR7 (CD197) BV421 2-L1-A (antibody)
RRID:AB_2869843
This monoclonal targets CCR7 (CD197)
View all literature mentionsHu CD279 (PD-1) BUV737 EH12.1 100Tst (antibody)
RRID:AB_2870118
This monoclonal targets CD279 (PD-1)
View all literature mentionsMouse Anti-Human CD197 Monoclonal Antibody, Alexa Fluor 647 Conjugated, Clone 150503 (antibody)
RRID:AB_2033948
This monoclonal targets Human CD197
View all literature mentionsCD185 (CXCR5) Antibody, anti-human, PE-Vio® 770, REAfinity™ (antibody)
RRID:AB_2733204
This recombinant monoclonal targets CD185 (CXCR5)
View all literature mentionsAPC anti-human CD137 (4-1BB) (antibody)
RRID:AB_830672
This monoclonal targets CD137
View all literature mentionsPE/Cyanine7 anti-human CD134 (OX40) (antibody)
RRID:AB_10901161
This monoclonal targets CD134
View all literature mentionsCD123 (IL-3 Receptor α) (antibody)
RRID:AB_2738038
This monoclonal targets CD123 (IL-3 Receptor α)
View all literature mentionsHu CD56 BUV563 NCAM16.2 100Tst (antibody)
RRID:AB_2870213
This monoclonal targets CD56 (NCAM-1)
View all literature mentionsFITC anti-human CD45RA (antibody)
RRID:AB_314410
This monoclonal targets CD45RA
View all literature mentionsHu CD45 BUV395 HI30 100Tst (antibody)
RRID:AB_2869519
This monoclonal targets CD45
View all literature mentionsBrilliant Violet 785(TM) anti-human CD20 (antibody)
RRID:AB_2566315
This monoclonal targets CD20
View all literature mentionsMouse Anti-CD19 Monoclonal Antibody, Phycoerythrin Conjugated, Clone HIB19 (antibody)
RRID:AB_395813
This monoclonal targets CD19
View all literature mentionsBV421 Mouse Anti-Human CD16 (antibody)
RRID:AB_2716865
This monoclonal targets CD16 (FcγRIII)
View all literature mentionsMouse Anti-CD11c Monoclonal Antibody, Phycoerythrin Conjugated, Clone B-ly6 (antibody)
RRID:AB_395793
This monoclonal targets CD11c
View all literature mentionsBV650 Mouse Anti-Human CD8 (antibody)
RRID:AB_2744462
This monoclonal targets CD8
View all literature mentionsMouse Anti-CD3 Monoclonal Antibody, FITC Conjugated, Clone HIT3a (antibody)
RRID:AB_395745
This monoclonal targets CD3
View all literature mentionsGoat anti-Human IgG (H+L) Secondary Antibody, HRP (antibody)
RRID:AB_228269
This polyclonal secondary targets IgG (H+L)
View all literature mentions
