Attenuated humoral responses in HIV after SARS-CoV-2 vaccination linked to B cell defects and altered immune profiles.
We assessed a cohort of people living with human immunodeficiency virus (PLWH) (n = 110) and HIV negative controls (n = 64) after 1, 2 or 3 SARS-CoV-2 vaccine doses. At all timepoints, PLWH had significantly lower neutralizing antibody (nAb) titers than HIV-negative controls. We also observed a delayed development of neutralization in PLWH that was underpinned by a reduced frequency of spike-specific memory B cells (MBCs). Improved neutralization breadth was seen against the Omicron variant (BA.1) after the third vaccine dose in PLWH but lower nAb responses persisted and were associated with global MBC dysfunction. In contrast, SARS-CoV-2 vaccination induced robust T cell responses that cross-recognized variants in PLWH. Strikingly, individuals with low or absent neutralization had detectable functional T cell responses. These PLWH had reduced numbers of circulating T follicular helper cells and an enriched population of CXCR3+CD127+CD8+T cells after two doses of SARS-CoV-2 vaccination.
Pubmed ID: 36590902 RIS Download
Research resources used in this publication
Additional research tools detected in this publication
None foundAntibodies used in this publication
- Anti-mouse Ig, k (RRID:AB_10051478)
- Cytofix/Cytoperm Soln Kit (RRID:AB_2869008)
- Mouse Anti-Human Interferon-gamma, Monoclonal Antibody, Biotinylated, clone 7-B6-1 (RRID:AB_907273)
- Mouse Anti-Human IFN-g (Interferon-gamma) Monoclonal Antibody, Unconjugated, Clone 1-D1K (RRID:AB_907282)
- APC anti-human CD19 (RRID:AB_314242)
- CD3 Monoclonal Antibody (OKT3), PerCP-eFluor™ 710, eBioscience (RRID:AB_1834395)
- CD183 (RRID:AB_394061)
- CD25 PE-Cy7 CE (RRID:AB_2868687)
- BV605 Mouse Anti-Human CD56 (RRID:AB_2728700)
- CD185 (CXCR5) (RRID:AB_2738871)
- Brilliant Violet 510(TM) anti-human CD19 (RRID:AB_2561668)
- Brilliant Violet 711(TM) anti-human CD8a (RRID:AB_2562906)
- PE/Dazzle(TM) 594 anti-human CD4 (RRID:AB_2563566)
- Brilliant Violet 421(TM) anti-human CD279 (PD-1) (RRID:AB_10960742)
- Alexa Fluor(R) 700 anti-human CD45RA (RRID:AB_493763)
- Brilliant Violet 785(TM) anti-human CD38 (RRID:AB_2565893)
- Brilliant Violet 711(TM) anti-human CD27 (RRID:AB_11219201)
- Brilliant Violet 650(TM) anti-human CD3 (RRID:AB_2563352)
- Brilliant Violet 650(TM) anti-human CD127 (IL-7Ralpha) (RRID:AB_11125369)
- APC/Cyanine7 anti-human CD197 (CCR7) (RRID:AB_10916390)
- Brilliant Violet 510(TM) anti-human CD14 (RRID:AB_2561946)
- Brilliant Violet 510(TM) anti-human CD3 (RRID:AB_2561943)
- CD38 (RRID:AB_11153122)
- CD21 (RRID:AB_2738040)
- Mouse Anti-Human CD20 Monoclonal Antibody, Alexa Fluor?? 700 Conjugated, Clone 2H7 (RRID:AB_1727447)
- APC/Cyanine7 anti-human IgM (RRID:AB_10900422)
- CD27 (RRID:AB_2744349)
- PE-Cy™7 Mouse Anti-Human IgD Clone IA6-2 (also known as δ-IA6-2) (RRID:AB_10642457)
- CD19 (RRID:AB_2740593)
- IgG (RRID:AB_10563419)
- Alkaline Phosphatase-AffiniPure Goat Anti-Human IgG, Fc_ Fragment Specific (min X Bov,Hrs,Ms Sr Prot) (RRID:AB_2337608)
- AffiniPure F(ab')2 Fragment Goat Anti-Human IgM, Fc5µ Fragment Specific (min X Bov Sr Prot) (RRID:AB_2337553)
Associated grants
- Agency: Medical Research Council, United Kingdom
Id: MC_PC_20060 - Agency: Medical Research Council, United Kingdom
Id: MR/W020556/1 - Agency: NIAID NIH HHS, United States
Id: R01 AI155182 - Agency: Medical Research Council, United Kingdom
Id: MR/T032413/1 - Agency: Medical Research Council, United Kingdom
Id: MR/R008698/1
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This is a list of tools and resources that we have found mentioned in this publication.
Anti-mouse Ig, k (antibody)
RRID:AB_10051478
This unknown targets mouse Ig k
View all literature mentionsCytofix/Cytoperm Soln Kit (antibody)
RRID:AB_2869008
This unknown targets
View all literature mentionsMouse Anti-Human Interferon-gamma, Monoclonal Antibody, Biotinylated, clone 7-B6-1 (antibody)
RRID:AB_907273
This monoclonal targets Mouse Human Interferon-gamma ylated clone 7-B6-1
View all literature mentionsMouse Anti-Human IFN-g (Interferon-gamma) Monoclonal Antibody, Unconjugated, Clone 1-D1K (antibody)
RRID:AB_907282
This monoclonal targets Human IFN-?? (Interferon-gamma)
View all literature mentionsAPC anti-human CD19 (antibody)
RRID:AB_314242
This monoclonal targets CD19
View all literature mentionsCD3 Monoclonal Antibody (OKT3), PerCP-eFluor™ 710, eBioscience (antibody)
RRID:AB_1834395
This monoclonal targets CD3
View all literature mentionsCD25 PE-Cy7 CE (antibody)
RRID:AB_2868687
This monoclonal targets CD25 (IL-2 Receptor α)
View all literature mentionsBV605 Mouse Anti-Human CD56 (antibody)
RRID:AB_2728700
This monoclonal targets CD56 (NCAM-1)
View all literature mentionsCD185 (CXCR5) (antibody)
RRID:AB_2738871
This monoclonal targets CD185 (CXCR5)
View all literature mentionsBrilliant Violet 510(TM) anti-human CD19 (antibody)
RRID:AB_2561668
This monoclonal targets CD19
View all literature mentionsBrilliant Violet 711(TM) anti-human CD8a (antibody)
RRID:AB_2562906
This monoclonal targets CD8a
View all literature mentionsPE/Dazzle(TM) 594 anti-human CD4 (antibody)
RRID:AB_2563566
This monoclonal targets CD4
View all literature mentionsBrilliant Violet 421(TM) anti-human CD279 (PD-1) (antibody)
RRID:AB_10960742
This monoclonal targets CD279
View all literature mentionsAlexa Fluor(R) 700 anti-human CD45RA (antibody)
RRID:AB_493763
This monoclonal targets CD45RA
View all literature mentionsBrilliant Violet 785(TM) anti-human CD38 (antibody)
RRID:AB_2565893
This monoclonal targets CD38
View all literature mentionsBrilliant Violet 711(TM) anti-human CD27 (antibody)
RRID:AB_11219201
This monoclonal targets CD27
View all literature mentionsBrilliant Violet 650(TM) anti-human CD3 (antibody)
RRID:AB_2563352
This monoclonal targets CD3
View all literature mentionsBrilliant Violet 650(TM) anti-human CD127 (IL-7Ralpha) (antibody)
RRID:AB_11125369
This monoclonal targets CD127
View all literature mentionsAPC/Cyanine7 anti-human CD197 (CCR7) (antibody)
RRID:AB_10916390
This monoclonal targets CD197
View all literature mentionsBrilliant Violet 510(TM) anti-human CD14 (antibody)
RRID:AB_2561946
This monoclonal targets CD14
View all literature mentionsBrilliant Violet 510(TM) anti-human CD3 (antibody)
RRID:AB_2561943
This monoclonal targets CD3
View all literature mentionsMouse Anti-Human CD20 Monoclonal Antibody, Alexa Fluor?? 700 Conjugated, Clone 2H7 (antibody)
RRID:AB_1727447
This monoclonal targets CD20
View all literature mentionsAPC/Cyanine7 anti-human IgM (antibody)
RRID:AB_10900422
This monoclonal targets IgM
View all literature mentionsPE-Cy™7 Mouse Anti-Human IgD Clone IA6-2 (also known as δ-IA6-2) (antibody)
RRID:AB_10642457
This monoclonal targets IgD
View all literature mentionsAlkaline Phosphatase-AffiniPure Goat Anti-Human IgG, Fc_ Fragment Specific (min X Bov,Hrs,Ms Sr Prot) (antibody)
RRID:AB_2337608
This unknown targets Human IgG, Fc? Fragment Specific
View all literature mentionsAffiniPure F(ab')2 Fragment Goat Anti-Human IgM, Fc5µ Fragment Specific (min X Bov Sr Prot) (antibody)
RRID:AB_2337553
This unknown targets Human IgM, Fc5
View all literature mentionsHEK293T/17 (cell line)
RRID:CVCL_1926
Cell line HEK293T/17 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentions
