High-sucrose diet exposure is associated with selective and reversible alterations in the rat peripheral taste system.
Elevated sugar consumption is associated with an increased risk for metabolic diseases. Whereas evidence from humans, rodents, and insects suggests that dietary sucrose modifies sweet taste sensation, understanding of peripheral nerve or taste bud alterations is sparse. To address this, male rats were given access to 30% liquid sucrose for 4 weeks (sucrose rats). Neurophysiological responses of the chorda tympani (CT) nerve to lingual stimulation with sugars, other taste qualities, touch, and cold were then compared with controls (access to water only). Morphological and immunohistochemical analyses of fungiform papillae and taste buds were also conducted. Sucrose rats had substantially decreased CT responses to 0.15-2.0 M sucrose compared with controls. In contrast, effects were not observed for glucose, fructose, maltose, Na saccharin, NaCl, organic acid, or umami, touch, or cold stimuli. Whereas taste bud number, size, and innervation volume were unaffected, the number of PLCβ2+ taste bud cells in the fungiform papilla was reduced in sucrose rats. Notably, the replacement of sucrose with water resulted in a complete recovery of all phenotypes over 4 weeks. The work reveals the selective and modality-specific effects of sucrose consumption on peripheral taste nerve responses and taste bud cells, with implications for nutrition and metabolic disease risk. VIDEO ABSTRACT.
Pubmed ID: 35977546 RIS Download
Research resources used in this publication
- IBM SPSS Statistics (RRID:SCR_019096) (software resource)
- Fiji (RRID:SCR_002285) (software resource)
- GraphPad Prism (RRID:SCR_002798) (software resource)
- Imaris (RRID:SCR_007370) (software resource)
- Huygens Software (RRID:SCR_014237) (software resource)
- Spike2 Software (RRID:SCR_000903) (software resource)
Additional research tools detected in this publication
None foundAntibodies used in this publication
- Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (RRID:AB_2534013)
- Donkey Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor ?? 488 Conjugated (RRID:AB_2535792)
- PLC beta2 (Q-15) (RRID:AB_632197)
- Anti-P2X3 Receptor Antibody (RRID:AB_2313760)
- Mouse Anti-Cytokeratin 8 + 18 + 19 Monoclonal Antibody, Unconjugated, Clone 2A4 (RRID:AB_736438)
Associated grants
- Agency: NIDCD NIH HHS, United States
Id: R01 DC014428 - Agency: NIDDK NIH HHS, United States
Id: R01 DK115526 - Agency: NIDDK NIH HHS, United States
Id: P30 DK089503 - Agency: NIDDK NIH HHS, United States
Id: R01 DK106188 - Agency: NIDDK NIH HHS, United States
Id: R00 DK097141 - Agency: NIDDK NIH HHS, United States
Id: P30 DK020572 - Agency: NIDDK NIH HHS, United States
Id: DP2 DK113750
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This is a list of tools and resources that we have found mentioned in this publication.
Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (antibody)
RRID:AB_2534013
This polyclonal secondary targets IgG (H+L)
View all literature mentionsDonkey Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor ?? 488 Conjugated (antibody)
RRID:AB_2535792
This unknown targets Rabbit IgG (H+L)
View all literature mentionsPLC beta2 (Q-15) (antibody)
RRID:AB_632197
This polyclonal targets PLC beta2 (Q-15)
View all literature mentionsAnti-P2X3 Receptor Antibody (antibody)
RRID:AB_2313760
This unknown targets P2X3 Receptor
View all literature mentionsMouse Anti-Cytokeratin 8 + 18 + 19 Monoclonal Antibody, Unconjugated, Clone 2A4 (antibody)
RRID:AB_736438
This monoclonal targets Cytokeratin 8 + 18 + 19
View all literature mentionsIBM SPSS Statistics (software resource)
RRID:SCR_019096
Software package for statistics. Used to analyze and visualize data. Extensions can be used, Python and R programming language code, to integrate with open source software. Available for Windows and Mac operating systems.Versions that were produced by SPSS Inc. before the IBM acquisition (Versions 18 and earlier) would be given origin or publisher of SPSS Inc. in Chicago. Versions that were released after the acquisition would be given origin or publisher of IBM Corp. in Armonk, NY.
View all literature mentionsFiji (software resource)
RRID:SCR_002285
Software package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsGraphPad Prism (software resource)
RRID:SCR_002798
Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsImaris (software resource)
RRID:SCR_007370
Imaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.
View all literature mentionsHuygens Software (software resource)
RRID:SCR_014237
A set of fluorescence microscope image processing packages which perform image restoration, interactive analysis, and volume visualization of 2D and 3D multi channel microscopy images or time series. The restoration is based on different deconvolution algorithms, that permit the recovery of objects from images that are degraded by blurring and noise. Tutorials and documentation are available on the website.
View all literature mentionsSpike2 Software (software resource)
RRID:SCR_000903
THIS RESOURCE IS NO LONGER IN SERVICE.Documented on September 23,2022. A data acquisition and analysis software package for electrophysiology data. Spike2 software offers multi-channel continuous data acquisition and analysis with a multitude of options. This offers flexible usage from a simple chart recorder to complex applications requiring stimulus generation, data capture, scrolling or triggered displays, control of external equipment, and custom analysis. Spike2 software can be used in many fields such as electrophysiology, neurophysiology, cardiovascular and respiratory studies, sports science and pharmacology.
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