Addgene (tool)

RRID:SCR_002037

Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.

Promega (tool)

RRID:SCR_006724

An Antibody supplier

Thermo Fisher Scientific (tool)

RRID:SCR_008452

Commercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.

Millipore (tool)

RRID:SCR_008983

An Antibody supplier

Proteintech Group (tool)

RRID:SCR_008986

Proteintech Europe Ltd is an ISO 9001:2008 certified company

Sigma-Aldrich (tool)

RRID:SCR_008988

American chemical, life science and biotechnology company owned by Merck KGaA. Merger of Sigma Chemical Company and Aldrich Chemical Company. Provides organic and inorganic chemicals, building blocks, reagents, advanced materials and stable isotopes for chemical synthesis, medicinal chemistry and materials science, antibiotics, buffers, carbohydrates, enzymes, forensic tools, hematology and histology, nucleotides, proteins, peptides, amino acids and their derivatives.

HEK293 (tool)

RRID:CVCL_0045

Cell line HEK293 is a Transformed cell line with a species of origin Homo sapiens (Human)

HEK293T (tool)

RRID:CVCL_0063

Cell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)

Flp-In-T-REx-293 (tool)

RRID:CVCL_U427

Cell line Flp-In-T-REx-293 is a Transformed cell line with a species of origin Homo sapiens (Human)

Flp-In-T-REx-293 (tool)

RRID:CVCL_U427

Cell line Flp-In-T-REx-293 is a Transformed cell line with a species of origin Homo sapiens (Human)

Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in goat (antibody)

RRID:AB_258284

This unknown targets Rabbit IgG, whole molecule

Sheep Anti-Mouse IgG - Horseradish Peroxidase (antibody)

RRID:AB_772210

This monoclonal secondary targets IgG

Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 (antibody)

RRID:AB_2534073

This polyclonal secondary targets IgG (H+L)

Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (antibody)

RRID:AB_143165

This polyclonal targets IgG (H+L)

6x-His Tag Monoclonal Antibody (HIS.H8), HRP (antibody)

RRID:AB_2536989

This monoclonal targets 6x-His Tag

V5 Tag Monoclonal Antibody (SV5-Pk1) (antibody)

RRID:AB_2556564

This monoclonal targets V5 Tag

Monoclonal ANTI-FLAG® M2 antibody produced in mouse (antibody)

RRID:AB_262044

This monoclonal targets FLAG

ZNF598 Recombinant Rabbit Monoclonal Antibody (5H5L17) (antibody)

RRID:AB_2815335

This recombinant monoclonal targets ZNF598

GAPDH antibody (antibody)

RRID:AB_2107436

This monoclonal targets GAPDH

EIF4E2 antibody (antibody)

RRID:AB_10642945

This polyclonal targets EIF4E2

CNOT9 antibody (antibody)

RRID:AB_11232413

This polyclonal targets CNOT9

DDX6 antibody (antibody)

RRID:AB_2091264

This polyclonal targets DDX6

GIGYF2 antibody (antibody)

RRID:AB_2879727

This polyclonal targets GIGYF2

Jalview (software resource)

RRID:SCR_006459

A free program for multiple sequence alignment editing, visualisation and analysis that is available in two forms: a lightweight Java applet for use in web applications, and a powerful desktop application that employs web services for sequence alignment, secondary structure prediction and the retrieval of alignments, sequences, annotation and structures from public databases and any DAS 1.53 compliant sequence or annotation server. Use it to view and edit sequence alignments, analyse them with phylogenetic trees and principal components analysis (PCA) plots and explore molecular structures and annotation. Jalview has built in DNA, RNA and protein sequence and structure visualisation and analysis capabilities. It uses Jmol to view 3D structures, and VARNA to display RNA secondary structure.

UCSF ChimeraX (software resource)

RRID:SCR_015872

Software for 3D/4D image reconstruction. UCSF ChimeraX is the next-generation molecular visualization program from the Resource for Biocomputing, Visualization, and Informatics (RBVI), following UCSF Chimera.

GraphPad Prism (software resource)

RRID:SCR_002798

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

pSpCas9(BB)-2A-Puro (PX459) V2.0 (plasmid)

RRID:Addgene_62988

pcDNA5-FRT-TO-FH-Nsp2 (plasmid)

RRID:Addgene_157683

pFRT/TO/FLAG/HA-DEST TNRC6C (plasmid)

RRID:Addgene_19885

Image Lab Software (software resource)

RRID:SCR_014210

Imaging software used to acquire and analyze images from specific Bio-Rad imaging systems. Users can analyze gel or blot features, capture optimized image data, and generate a report of the data. Image Lab software exclusively runs on the Gel Doc EZ imager, Gel Doc XR+ imaging system, ChemiDo MP, ChemiDoc XRS+ imaging systems, Criterion Stain Free imager, and the GS-900calibrated densitometer.

Adobe Illustrator (software resource)

RRID:SCR_010279

Vector graphics software to create digital graphics, illustrations, and typography for several types of media: print, web, interactive, video, and mobile.

Fiji (software resource)

RRID:SCR_002285

Software package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.