X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands.

Olivier Mauduit | Marit H Aure | Vanessa Delcroix | Liana Basova | Amrita Srivastava | Takeshi Umazume | Jacqueline W Mays | Saverio Bellusci | Abigail S Tucker | Mohammad K Hajihosseini | Matthew P Hoffman | Helen P Makarenkova
Cell reports | 2022

Fibroblast growth factor 10 (FGF10) is well established as a mesenchyme-derived growth factor and a critical regulator of fetal organ development in mice and humans. Using a single-cell RNA sequencing (RNA-seq) atlas of salivary gland (SG) and a tamoxifen inducible Fgf10CreERT2:R26-tdTomato mouse, we show that FGF10pos cells are exclusively mesenchymal until postnatal day 5 (P5) but, after P7, there is a switch in expression and only epithelial FGF10pos cells are observed after P15. Further RNA-seq analysis of sorted mesenchymal and epithelial FGF10pos cells shows that the epithelial FGF10pos population express the hallmarks of ancient ionocyte signature Forkhead box i1 and 2 (Foxi1, Foxi2), Achaete-scute homolog 3 (Ascl3), and the cystic fibrosis transmembrane conductance regulator (Cftr). We propose that epithelial FGF10pos cells are specialized SG ionocytes located in ducts and important for the ionic modification of saliva. In addition, they maintain FGF10-dependent gland homeostasis via communication with FGFR2bpos ductal and myoepithelial cells.

Pubmed ID: 35417692 RIS Download

Associated grants

  • Agency: NIDCR NIH HHS, United States
    Id: R01 DE031044
  • Agency: NEI NIH HHS, United States
    Id: R01 EY026202
  • Agency: NEI NIH HHS, United States
    Id: R01 EY028983

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


circlize (tool)

RRID:SCR_002141

Software package that implements and enhances circular visualization in R. Due to natural born feature of R to draw statistical graphics, this package can provide more general and flexible way to visualize huge information in circular style.

View all literature mentions

STAR (tool)

RRID:SCR_004463

Software performing alignment of high-throughput RNA-seq data. Aligns RNA-seq reads to reference genome using uncompressed suffix arrays.

View all literature mentions

RSeQC (tool)

RRID:SCR_005275

Software package to comprehensively evaluate different aspects of RNA-seq experiments, such as sequence quality, GC bias, polymerase chain reaction bias, nucleotide composition bias, sequencing depth, strand specificity, coverage uniformity and read distribution over the genome structure. RSeQC takes both SAM and BAM files as input, which can be produced by most RNA-seq mapping tools as well as BED files, which are widely used for gene models.

View all literature mentions

HTSeq (tool)

RRID:SCR_005514

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on February 28,2023. Software Python package that provides infrastructure to process data from high-throughput sequencing assays. While the main purpose of HTSeq is to allow you to write your own analysis scripts, customized to your needs, there are also a couple of stand-alone scripts for common tasks that can be used without any Python knowledge.

View all literature mentions

HPA (tool)

RRID:SCR_006710

Public database with millions of high-resolution images showing the spatial distribution of proteins in different normal human tissues and cancer types, as well as different human cell lines. The data is released together with application-specific validation performed for each antibody, including immunohistochemisty, Western blot analysis and, for a large fraction, a protein array assay and immunofluorescent based confocal microscopy. The database has been developed in a gene-centric manner with the inclusion of all human genes predicted from genome efforts. Search functionalities allow for complex queries regarding protein expression profiles, protein classes and chromosome location. Antibodies included have been analyzed using a standardized protocol in a single attempt without further efforts to optimize the procedure and therefore it cannot be excluded that certain observed binding properties are due to technical rather than biological reasons and that further optimization could result in a different outcome. Submission of antibodies: The Swedish Human Proteome Atlas (HPA) program, invites submission of antibodies from both academic and commercial sources to be included in the human protein atlas. All antibodies will be validated by the HPA-program by a standard procedure and antibodies that are accepted will be use in the tissue- profiling program to generate high-resolution immunohistochemistry images representing a wide spectrum of normal tissues and cancer types.

View all literature mentions

SEURAT (tool)

RRID:SCR_007322

A software tool which provides interactive visualization capability for the integrated analysis of high-dimensional gene expression data.

View all literature mentions

Thermo Fisher Scientific (tool)

RRID:SCR_008452

Commercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.

View all literature mentions

FlowJo (tool)

RRID:SCR_008520

Software for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.

View all literature mentions

Molecular Probes (tool)

RRID:SCR_013318

An Antibody supplier and subset of ThermoFisher Scientific which provides fluorescence reagents for various experiments and methods.

View all literature mentions

DESeq2 (tool)

RRID:SCR_015687

Software package for differential gene expression analysis based on the negative binomial distribution. Used for analyzing RNA-seq data for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates.

View all literature mentions

R Project for Statistical Computing (tool)

RRID:SCR_001905

Software environment and programming language for statistical computing and graphics. R is integrated suite of software facilities for data manipulation, calculation and graphical display. Can be extended via packages. Some packages are supplied with the R distribution and more are available through CRAN family.It compiles and runs on wide variety of UNIX platforms, Windows and MacOS.

View all literature mentions

BioVision (tool)

RRID:SCR_005057

An Antibody supplier

View all literature mentions

C57BL/6J (tool)

RRID:IMSR_JAX:000664

Mus musculus with name C57BL/6J from IMSR.

View all literature mentions

CD31 (antibody)

RRID:AB_10612003

This monoclonal targets CD31

View all literature mentions

Vimentin antibody (antibody)

RRID:AB_2881439

This monoclonal targets Vimentin

View all literature mentions

Anti-PANX1 antibody produced in rabbit (antibody)

RRID:AB_1854954

This polyclonal targets PANX1 antibody produced in rabbit

View all literature mentions

NKCC1 (N-16) (antibody)

RRID:AB_2188633

This polyclonal targets SLC12A2

View all literature mentions

Cytokeratin 7 antibody [RCK105] (antibody)

RRID:AB_306947

This monoclonal targets Cytokeratin 7 antibody [RCK105]

View all literature mentions

Anti-FOXI1 antibody produced in rabbit (antibody)

RRID:AB_10761468

This polyclonal targets FOXI1 antibody produced in rabbit

View all literature mentions

E-Cadherin (antibody)

RRID:AB_397581

This monoclonal targets E-Cadherin

View all literature mentions

Mouse Anti-Human Glyceraldehyde 3-phosphate Dehydrogenase (GAPDH) Monoclonal, Unconjugated (antibody)

RRID:AB_900923

This monoclonal targets Human Glyceraldehyde 3-phosphate Dehydrogenase (GAPDH)

View all literature mentions

Aquaporin 5 antibody [EPR3747] (antibody)

RRID:AB_2049171

This monoclonal targets Aquaporin 5 antibody [EPR3747]

View all literature mentions

Monoclonal Anti-Actin, α-Smooth Muscle (antibody)

RRID:AB_476701

This monoclonal targets Actin, α-Smooth Muscle

View all literature mentions

B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (organism)

RRID:IMSR_JAX:007909

Mus musculus with name B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J from IMSR.

View all literature mentions