ATP6V1H deficiency impairs glucose tolerance by augmenting endoplasmic reticulum stress in high fat diet fed mice.
Vacuolar H+-ATPase (V-ATPase) is a ubiquitous proton pump that mediates the proton transmembrane transportation in various cells. Previously, H subunit of V-ATPase (ATP6V1H) was found to be related with insulin secretion and diabetes. However, the mechanism by which ATP6V1H regulates insulin secretion and glucose metabolism remains unclear. Herein, we established a high-fat-diet (HFD) fed model with Atp6v1h+/- mice and detected the expression and secretion of insulin and some biochemical indices of glucose metabolism, in order to explore the related mechanisms in β-cells. Transcriptome sequencing, qPCR and western blot analysis showed that ATP6V1H deficiency worsened fatty acid-induced glucose tolerance impairment by augmenting endoplasmic reticulum stress in β-cells, and alternative splicing of ATP6V1H might be involved in this process. These results indicated that ATP6V1H deficiency increased the susceptibility to T2DM.
Pubmed ID: 34990584 RIS Download
Research resources used in this publication
Additional research tools detected in this publication
None foundAntibodies used in this publication
- ATG5 antibody (RRID:AB_2062045)
- Beclin 1 antibody (RRID:AB_2259061)
- CHOP; GADD153 antibody (RRID:AB_2292610)
- Anti-Insulin Antibody [EPR17359] (RRID:AB_2716761)
- LC3B Antibody (RRID:AB_915950)
- Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) (RRID:AB_2827756)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (RRID:AB_2630356)
- Anti-Insulin antibody (RRID:AB_2877638)
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This is a list of tools and resources that we have found mentioned in this publication.
Beclin 1 antibody (antibody)
RRID:AB_2259061
This polyclonal targets Beclin 1
View all literature mentionsCHOP; GADD153 antibody (antibody)
RRID:AB_2292610
This polyclonal targets CHOP; GADD153
View all literature mentionsAnti-Insulin Antibody [EPR17359] (antibody)
RRID:AB_2716761
This monoclonal targets Insulin
View all literature mentionsGoat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) (antibody)
RRID:AB_2827756
This polyclonal targets IgG
View all literature mentionsGoat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (antibody)
RRID:AB_2630356
This polyclonal secondary targets IgG (H+L)
View all literature mentionsAnti-Insulin antibody (antibody)
RRID:AB_2877638
This polyclonal targets insulin
View all literature mentionsMIN6 (cell line)
RRID:CVCL_0431
Cell line MIN6 is a Transformed cell line with a species of origin Mus musculus
View all literature mentions
