Aging Does Not Exacerbate Muscle Loss During Denervation and Lends Unique Muscle-Specific Atrophy Resistance With Akt Activation.
Sarcopenia, or age-related skeletal muscle atrophy and weakness, imposes significant clinical and economic burdens on affected patients and societies. Neurological degeneration, such as motoneuron death, has been recognized as a key contributor to sarcopenia. However, little is known about how aged/sarcopenic muscle adapts to this denervation stress. Here, we show that mice at 27months of age exhibit clear signs of sarcopenia but no accelerated denervation-induced muscle atrophy when compared to 8-month-old mice. Surprisingly, aging lends unique atrophy resistance to tibialis anteria muscle, accompanied by an increase in the cascade of mammalian target of rapamycin complex 1 (mTORC1)-independent anabolic events involving Akt signaling, rRNA biogenesis, and protein synthesis during denervation. These results expand our understanding of age-dependent stress responses and may help develop better countermeasures to sarcopenia.
Pubmed ID: 34916960 RIS Download
Research resources used in this publication
None foundAdditional research tools detected in this publication
Antibodies used in this publication
- IRS-1 Antibody (RRID:AB_330333)
- GSK-3beta (3D10) Mouse mAb (RRID:AB_10839406)
- Pan-Actin (D18C11) Rabbit mAb (RRID:AB_10998774)
- IGF-IRbeta (C-20) (RRID:AB_671792)
- Peroxidase-AffiniPure Goat Anti-Mouse IgG, Fc_ Subclass 2a Specific (min X Hu,Bov,Rb Sr Prot) (RRID:AB_2338514)
- Rabbit anti-Goat IgG (H+L), Superclonal Recombinant Secondary Antibody, HRP (RRID:AB_2536079)
- Peroxidase-AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) (RRID:AB_2337942)
- Goat F(ab')2 anti-Mouse IgG (H+L)-HRPO (RRID:AB_2617176)
- PTEN Antibody (RRID:AB_10694066)
- Anti-PI3 Kinase p85 Antibody, Unconjugated (RRID:AB_329869)
- Phospho-IGF-I Receptor beta (Tyr1135/1136)/Insulin Receptor beta (Tyr1150/1151) (19H7) Rabbit mAb (RRID:AB_331253)
- Phospho-GSK-3 (Ser9) (D85E12) XP Rabbit mAb (RRID:AB_10013750)
- Phospho-Akt (Ser473) Antibody (RRID:AB_329825)
- Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb (RRID:AB_2629447)
- Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (RRID:AB_2315112)
- Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP Rabbit mAb (RRID:AB_10694233)
- p70 S6 Kinase Antibody (RRID:AB_331676)
- Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb (RRID:AB_2269803)
- K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb (RRID:AB_10859893)
- Anti-Puromycin Antibody, clone 12D10 (RRID:AB_2566826)
- p-IRS-1 (Tyr 632) (RRID:AB_669445)
- Ub Antibody (P4D1) (RRID:AB_2762364)
Associated grants
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This is a list of tools and resources that we have found mentioned in this publication.
GSK-3beta (3D10) Mouse mAb (antibody)
RRID:AB_10839406
This monoclonal targets GSK-3beta (3D10) Mouse mAb
View all literature mentionsPan-Actin (D18C11) Rabbit mAb (antibody)
RRID:AB_10998774
This monoclonal targets Pan-Actin (D18C11) Rabbit mAb
View all literature mentionsIGF-IRbeta (C-20) (antibody)
RRID:AB_671792
This unknown targets Epitope maps to carboxy terminus of IGF-!R of human origin. Specific for IGF-IRbeta, Non-reactive with IGF-Iralpha
View all literature mentionsPeroxidase-AffiniPure Goat Anti-Mouse IgG, Fc_ Subclass 2a Specific (min X Hu,Bov,Rb Sr Prot) (antibody)
RRID:AB_2338514
This unknown targets Mouse IgG, Fc? Subclass 2a Specific
View all literature mentionsRabbit anti-Goat IgG (H+L), Superclonal Recombinant Secondary Antibody, HRP (antibody)
RRID:AB_2536079
This recombinant polyclonal secondary targets IgG (H+L)
View all literature mentionsPeroxidase-AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) (antibody)
RRID:AB_2337942
This unknown targets Rabbit IgG (H+L)
View all literature mentionsGoat F(ab')2 anti-Mouse IgG (H+L)-HRPO (antibody)
RRID:AB_2617176
This polyclonal targets Mouse IgG (H+L)
View all literature mentionsAnti-PI3 Kinase p85 Antibody, Unconjugated (antibody)
RRID:AB_329869
This unknown targets PI3 Kinase p85
View all literature mentionsPhospho-IGF-I Receptor beta (Tyr1135/1136)/Insulin Receptor beta (Tyr1150/1151) (19H7) Rabbit mAb (antibody)
RRID:AB_331253
This monoclonal targets Phospho-IGF-I Receptor beta (Tyr1135/1136)/Insulin Receptor beta (Tyr1150/1151) (19H7) Rabbit mAb
View all literature mentionsPhospho-GSK-3 (Ser9) (D85E12) XP Rabbit mAb (antibody)
RRID:AB_10013750
This monoclonal targets Phospho-GSK-3 (Ser9)
View all literature mentionsPhospho-Akt (Ser473) Antibody (antibody)
RRID:AB_329825
This polyclonal targets Phospho-Akt (Ser473)
View all literature mentionsPhospho-Akt (Thr308) (D25E6) XP® Rabbit mAb (antibody)
RRID:AB_2629447
This monoclonal targets
View all literature mentionsPhospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (antibody)
RRID:AB_2315112
This monoclonal targets Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
View all literature mentionsPhospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP Rabbit mAb (antibody)
RRID:AB_10694233
This monoclonal targets Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP Rabbit mAb
View all literature mentionsp70 S6 Kinase Antibody (antibody)
RRID:AB_331676
This polyclonal targets p70 S6 Kinase
View all literature mentionsPhospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb (antibody)
RRID:AB_2269803
This monoclonal targets p70 S6 Kinase, phospho (Thr389)
View all literature mentionsK48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb (antibody)
RRID:AB_10859893
This monoclonal targets K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb
View all literature mentionsAnti-Puromycin Antibody, clone 12D10 (antibody)
RRID:AB_2566826
This monoclonal targets Puromycin from Streptomyces alboniger
View all literature mentionsp-IRS-1 (Tyr 632) (antibody)
RRID:AB_669445
This polyclonal targets p-IRS-1 (Tyr 632)
View all literature mentionsUb Antibody (P4D1) (antibody)
RRID:AB_2762364
This monoclonal targets Ub
View all literature mentions
