Generation of integration-free human iPSC line LCPHi001-A from a Parkinson's disease patient carrying the RecNciI mutation in GBA gene.
Parkinson's disease (PD) is a neurodegenerative disease caused by environmental and genetic factors. The identified PD genes include SNCA, LRRK2, Parkin, DJ-1, PINK1, and ATP13A2. Mutations in the glucocerebrosidase (GBA) gene were reported to be associated with PD in different ethnic populations. Here we generated a novel induced pluripotent stem cell (iPSC) line LCPHi001-A from a PD patient carrying RecNciI mutation (c.1448 T > C, c.1483G > C, and c.1497G > C) in GBA by non-integrative episomal plasmids. The LCPHi001-A line expressed pluripotency markers, displayed differentiation capacity to three germ layers in vivo, and had the normal karyotype.
Pubmed ID: 34464855 RIS Download
Research resources used in this publication
None foundAdditional research tools detected in this publication
None foundAntibodies used in this publication
- Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot) (RRID:AB_2307443)
- Alexa Fluor 488-AffiniPure Donkey Anti-Mouse IgM, µ Chain Specific (RRID:AB_2340844)
- Alexa Fluor 488-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot) (RRID:AB_2340846)
- NEW Anti-NANOG, clone 7F7.1 (RRID:AB_11203826)
- Anti-TRA-1-60, clone TRA-1-60 (RRID:AB_2119183)
- Anti-SOX2 antibody (RRID:AB_2814892)
- Anti-Oct-4 (RRID:AB_2167706)
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This is a list of tools and resources that we have found mentioned in this publication.
Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot) (antibody)
RRID:AB_2307443
This polyclonal targets IgG (H+L)
View all literature mentionsAlexa Fluor 488-AffiniPure Donkey Anti-Mouse IgM, µ Chain Specific (antibody)
RRID:AB_2340844
This unknown targets Mouse IgM,
View all literature mentionsAlexa Fluor 488-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot) (antibody)
RRID:AB_2340846
This unknown targets Mouse IgG (H+L)
View all literature mentionsNEW Anti-NANOG, clone 7F7.1 (antibody)
RRID:AB_11203826
This monoclonal targets NEW NANOG clone 7F7.1
View all literature mentionsAnti-TRA-1-60, clone TRA-1-60 (antibody)
RRID:AB_2119183
This monoclonal targets HSP90B1
View all literature mentionsAnti-SOX2 antibody (antibody)
RRID:AB_2814892
This polyclonal targets SOX2
View all literature mentions
