Algorithmic assessment of cellular senescence in experimental and clinical specimens.
The development of genetic tools allowed for the validation of the pro-aging and pro-disease functions of senescent cells in vivo. These discoveries prompted the development of senotherapies-pharmaceutical interventions aimed at interfering with the detrimental effect of senescent cells-that are now entering the clinical stage. However, unequivocal identification and examination of cellular senescence remains highly difficult because of the lack of universal and specific markers. Here, to overcome the limitation of measuring individual markers, we describe a detailed two-phase algorithmic assessment to quantify various senescence-associated parameters in the same specimen. In the first phase, we combine the measurement of lysosomal and proliferative features with the expression of general senescence-associated genes to validate the presence of senescent cells. In the second phase we measure the levels of pro-inflammatory markers for specification of the type of senescence. The protocol can help graduate-level basic scientists to improve the characterization of senescence-associated phenotypes and the identification of specific senescent subtypes. Moreover, it can serve as an important tool for the clinical validation of the role of senescent cells and the effectiveness of anti-senescence therapies.
Pubmed ID: 33911261 RIS Download
Research resources used in this publication
Additional research tools detected in this publication
None foundAntibodies used in this publication
- Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 568 Conjugated (RRID:AB_2534072)
- Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, AP (RRID:AB_2536528)
- Anti-Biotin antibody [Hyb-8] (RRID:AB_2861249)
- VECTASTAIN ABC-Peroxidase Kit (Standard) (RRID:AB_2336818)
- Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated (RRID:AB_2313606)
- p21 (M-19) (RRID:AB_632123)
- Recombinant Anti-Ki67 antibody [SP6] (RRID:AB_302459)
Associated grants
- Agency: NIA NIH HHS, United States
Id: R01 AG051729 - Agency: NIA NIH HHS, United States
Id: K99 AG065484 - Agency: NIH HHS, United States
Id: S10 OD028654 - Agency: NIA NIH HHS, United States
Id: U01 AG060906 - Agency: NIA NIH HHS, United States
Id: P01 AG017242
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This is a list of tools and resources that we have found mentioned in this publication.
Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 568 Conjugated (antibody)
RRID:AB_2534072
This unknown targets IgG (H+L)
View all literature mentionsGoat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, AP (antibody)
RRID:AB_2536528
This polyclonal secondary targets IgG (H+L)
View all literature mentionsAnti-Biotin antibody [Hyb-8] (antibody)
RRID:AB_2861249
This monoclonal targets Biotin
View all literature mentionsVECTASTAIN ABC-Peroxidase Kit (Standard) (antibody)
RRID:AB_2336818
This unknown targets
View all literature mentionsGoat Anti-Rabbit IgG Antibody (H+L), Biotinylated (antibody)
RRID:AB_2313606
This polyclonal targets IgG
View all literature mentionsp21 (M-19) (antibody)
RRID:AB_632123
This polyclonal targets p21 (M-19)
View all literature mentionsRecombinant Anti-Ki67 antibody [SP6] (antibody)
RRID:AB_302459
This recombinant monoclonal targets Ki67
View all literature mentionsBJ [Human fibroblast] (cell line)
RRID:CVCL_3653
Cell line BJ [Human fibroblast] is a Finite cell line with a species of origin Homo sapiens (Human)
View all literature mentions
