Human primed ILCPs support endothelial activation through NF-κB signaling.
Innate lymphoid cells (ILCs) represent the most recently identified subset of effector lymphocytes, with key roles in the orchestration of early immune responses. Despite their established involvement in the pathogenesis of many inflammatory disorders, the role of ILCs in cancer remains poorly defined. Here we assessed whether human ILCs can actively interact with the endothelium to promote tumor growth control, favoring immune cell adhesion. We show that, among all ILC subsets, ILCPs elicited the strongest upregulation of adhesion molecules in endothelial cells (ECs) in vitro, mainly in a contact-dependent manner through the tumor necrosis factor receptor- and RANK-dependent engagement of the NF-κB pathway. Moreover, the ILCP-mediated activation of the ECs resulted to be functional by fostering the adhesion of other innate and adaptive immune cells. Interestingly, pre-exposure of ILCPs to human tumor cell lines strongly impaired this capacity. Hence, the ILCP-EC interaction might represent an attractive target to regulate the immune cell trafficking to tumor sites and, therefore, the establishment of an anti-tumor immune response.
Pubmed ID: 33554861 RIS Download
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- NEW Anti-ROR gamma T, clone 6F3.1 (RRID:AB_11205416)
- PECAM-1 (JC70) (RRID:AB_629040)
- Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor ?? 647 Conjugated (RRID:AB_2535804)
- Lymphotoxin Alpha + Beta antibody [MM0460-12B4] (RRID:AB_2050404)
- Brilliant Violet 711(TM) anti-human CD154 (RRID:AB_2563845)
- Brilliant Violet 650(TM) anti-human CD69 (RRID:AB_2563158)
- Brilliant Violet 650(TM) anti-human CD62L (RRID:AB_2561461)
- Brilliant Violet 650(TM) anti-human CD196 (CCR6) (RRID:AB_2562235)
- Brilliant Violet 421(TM) anti-human CD304 (Neuropilin-1) (RRID:AB_2562361)
- Brilliant Violet 421(TM) anti-human CD127 (IL-7Ralpha) (RRID:AB_10960140)
- Brilliant Violet 421(TM) anti-human CD185 (CXCR5) (RRID:AB_2562302)
- Pacific Blue(TM) anti-human CD54 (RRID:AB_10900234)
- CD73 Monoclonal Antibody (AD2), eFluor™ 450, eBioscience (RRID:AB_11041811)
- APC/Fire(TM) 750 anti-human CD45RO (RRID:AB_2616717)
- Mouse Anti-Human CD19 Antibody, APC-H7 Conjugated (RRID:AB_1645728)
- APC/Cyanine7 anti-human CD185 (CXCR5) (RRID:AB_2562593)
- Mouse Anti-Human CD45RA Monoclonal Antibody, Alexa Fluor?? 700 Conjugated, Clone HI100 (RRID:AB_1727496)
- Alexa Fluor(R) 700 anti-human CD16 (RRID:AB_2278418)
- Alexa Fluor(R) 700 anti-human CD4 (RRID:AB_493743)
- APC anti-GATA3 (RRID:AB_2562725)
- Mouse Anti-CD117 Monoclonal Antibody, Allophycocyanin Conjugated, Clone YB5.B8 (RRID:AB_398461)
- Mouse Anti-Human Cd30 ligand / tnfsf8 Monoclonal antibody, Allophycocyanin Conjugated, Clone 116614 (RRID:AB_416825)
- Anti-Human CD3 (TCR complex) Monoclonal Antibody, Allophycocyanin Conjugated, Clone UCHT1 (RRID:AB_130788)
- Nkp46 (RRID:AB_10894195)
- PE/Cyanine7 anti-human CD62E (RRID:AB_2800891)
- PE/Cyanine7 anti-human CD196 (CCR6) (RRID:AB_10916518)
- PE/Cyanine5 anti-human CD106 (RRID:AB_2214227)
- PerCP/Cyanine5.5 anti-human CD336 (NKp44) (RRID:AB_2616752)
- PerCP/Cyanine5.5 anti-human CD28 (RRID:AB_2073718)
- PerCP/Cyanine5.5 anti-human CD194 (CCR4) (RRID:AB_2562391)
- PE/Dazzle(TM) 594 anti-human CD39 (RRID:AB_2564318)
- T-bet (RRID:AB_2737621)
- CD14 (RRID:AB_11153663)
- PE Mouse anti-Human RORγt (RRID:AB_2686896)
- Human RANK/TNFRSF11A Phycoerythrin MAb (Clone 80704) (RRID:AB_10643566)
- PE anti-human CD254 (TRANCE, RANKL) (RRID:AB_2256265)
- IDO Monoclonal Antibody (eyedio), PE, eBioscience (RRID:AB_2572712)
- PE anti-human CD294 (CRTH2) (RRID:AB_10900060)
- PE anti-human CD62E (RRID:AB_536008)
- PE anti-human CD4 (RRID:AB_2562053)
- PE anti-human CD257 (BAFF, BLYS) (RRID:AB_830752)
- FITC anti-human FcepsilonRIalpha (RRID:AB_1227653)
- FITC anti-human CD203c (E-NPP3) (RRID:AB_11218991)
- CD94-FITC, human (RRID:AB_2659623)
- FITC anti-human CD34 (RRID:AB_1732005)
- FITC anti-human CD33 (RRID:AB_314344)
- FITC anti-human CD31 (RRID:AB_314330)
- FITC anti-human CD20 (RRID:AB_314252)
- FITC anti-human CD19 (RRID:AB_2750099)
- FITC anti-human CD16 (RRID:AB_314206)
- FITC anti-human CD15 (SSEA-1) (RRID:AB_314196)
- FITC anti-human CD14 (RRID:AB_2571929)
- FITC anti-human CD8 (RRID:AB_1877178)
- FITC anti-human CD4 (RRID:AB_2562052)
- FITC anti-human CD3 (RRID:AB_2562046)
- Alexa Fluor(R) 488 anti-human CD183 (CXCR3) (RRID:AB_10962442)
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RRID:AB_1645728
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RRID:AB_2562593
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RRID:AB_2256265
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RRID:AB_2562046
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RRID:AB_10962442
This monoclonal targets CD183
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RRID:SCR_002798
Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
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Cell line TCCSUP is a Cancer cell line with a species of origin Homo sapiens (Human)
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