The chromatin-remodeling protein BAF60/SWP73A regulates the plant immune receptor NLRs.
In both plant and animal innate immune responses, surveillance of pathogen infection is mediated by membrane-associated receptors and intracellular nucleotide-binding domain and leucine-rich-repeat receptors (NLRs). Homeostasis of NLRs is under tight multilayered regulation to avoid over-accumulation or over-activation, which often leads to autoimmune responses that have detrimental effects on growth and development. How NLRs are regulated epigenetically at the chromatin level remains unclear. Here, we report that SWP73A, an ortholog of the mammalian switch/sucrose nonfermentable (SWI/SNF) chromatin-remodeling protein BAF60, suppresses the expression of NLRs either directly by binding to the NLR promoters or indirectly by affecting the alternative splicing of some NLRs through the suppression of cell division cycle 5 (CDC5), a key regulator of RNA splicing. Upon infection, bacteria-induced small RNAs silence SWP73A to activate a group of NLRs and trigger robust immune responses. SWP73A may function as a H3K9me2 reader to enhance transcription suppression.
Pubmed ID: 33548199 RIS Download
Research resources used in this publication
None foundAdditional research tools detected in this publication
Antibodies used in this publication
- Anti-DYKDDDDK Affinity Gel (Binds the same epitope as Sigma's Anti-FLAG M2 Antibody) - 200-350-383 (RRID:AB_10704031)
- goat anti-rabbit IgG-HRP (RRID:AB_631747)
- Goat Anti-Mouse Goat anti-mouse IgG-HRP Polyclonal, Hrp Conjugated (RRID:AB_631736)
- Histone H3 (acetyl K9) antibody - ChIP Grade (RRID:AB_297491)
- Rabbit Anti-Histone H3, trimethyl (Lys27) Polyclonal antibody, Unconjugated (RRID:AB_310624)
- Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (RRID:AB_449854)
- Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (RRID:AB_306649)
- Monoclonal Anti-alpha-Tubulin antibody produced in mouse (RRID:AB_477582)
- Monoclonal ANTI-FLAG® M2 antibody (RRID:AB_259529)
Associated grants
- Agency: NIGMS NIH HHS, United States
Id: R01 GM093008 - Agency: NIGMS NIH HHS, United States
Id: R35 GM136379
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This is a list of tools and resources that we have found mentioned in this publication.
MACS (tool)
RRID:SCR_013291
Software Python package for identifying transcript factor binding sites. Used to evaluate significance of enriched ChIP regions. Improves spatial resolution of binding sites through combining information of both sequencing tag position and orientation. Can be used for ChIP-Seq data alone, or with control sample with increase of specificity.
View all literature mentionsBowtie 2 (tool)
RRID:SCR_016368
Ultrafast and memory efficient tool for aligning sequencing reads to long reference sequences. Supports gapped, local, and paired end alignment modes. More suited to finding longer, gapped alignments in comparison with original Bowtie method.
View all literature mentionsAnti-DYKDDDDK Affinity Gel (Binds the same epitope as Sigma's Anti-FLAG M2 Antibody) - 200-350-383 (antibody)
RRID:AB_10704031
This monoclonal targets Anti-DYKDDDDK (FLAG tag) Affinity Gel
View all literature mentionsgoat anti-rabbit IgG-HRP (antibody)
RRID:AB_631747
This polyclonal targets Rabbit IgG
View all literature mentionsGoat Anti-Mouse Goat anti-mouse IgG-HRP Polyclonal, Hrp Conjugated (antibody)
RRID:AB_631736
This polyclonal targets Mouse IgG
View all literature mentionsHistone H3 (acetyl K9) antibody - ChIP Grade (antibody)
RRID:AB_297491
This polyclonal targets Histone H3 (acetyl K9) antibody - ChIP Grade
View all literature mentionsRabbit Anti-Histone H3, trimethyl (Lys27) Polyclonal antibody, Unconjugated (antibody)
RRID:AB_310624
This polyclonal targets Histone H3, trimethyl (Lys27)
View all literature mentionsHistone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (antibody)
RRID:AB_449854
This monoclonal targets Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade
View all literature mentionsAnti-Histone H3 (tri methyl K4) antibody - ChIP Grade (antibody)
RRID:AB_306649
This polyclonal targets Histone H3 (tri methyl K4)
View all literature mentionsMonoclonal Anti-alpha-Tubulin antibody produced in mouse (antibody)
RRID:AB_477582
This monoclonal targets alpha-Tubulin antibody produced in mouse
View all literature mentionsMonoclonal ANTI-FLAG® M2 antibody (antibody)
RRID:AB_259529
This monoclonal targets FLAG
View all literature mentions
