Dynamin-2 Regulates Postsynaptic Cytoskeleton Organization and Neuromuscular Junction Development.
Neuromuscular junctions (NMJs) govern efficient neuronal communication with muscle cells, relying on proper architecture of specialized postsynaptic compartments. However, the intrinsic mechanism in muscle cells contributing to NMJ development remains unclear. In this study, we reveal that dynamin-2 (Dyn2) is involved in postsynaptic development of NMJs. Mutations of Dyn2 have been linked to human muscular disorder and centronuclear myopathy (CNM), as well as featured with muscle atrophy and defective NMJs, yet the function of Dyn2 at the postsynaptic membrane is largely unknown. We demonstrate that Dyn2 is enriched at the postsynaptic membrane and regulates NMJ development via actin remodeling. Dyn2 functions as an actin-bundling GTPase to regulate podosome turnover and cytoskeletal organization of the postsynaptic apparatus, and CNM-Dyn2 mutations display abnormal actin remodeling and electrophysiological activity of fly NMJs. Altogether, Dyn2 primarily regulates actin cytoskeleton remodeling and NMJ morphogenesis at the postsynaptic membrane, which is distinct from its endocytosis regulatory role at the presynaptic membrane.
Pubmed ID: 33113375 RIS Download
Research resources used in this publication
- C2C12 (RRID:CVCL_0188) (cell line)
- pClamp (RRID:SCR_011323) (software resource)
- ZEISS ZEN Microscopy Software (RRID:SCR_013672) (software resource)
- GraphPad Prism (RRID:SCR_002798) (software resource)
- ImageJ (RRID:SCR_003070) (software resource)
- MetaMorph Microscopy Automation and Image Analysis Software (RRID:SCR_002368) (software resource)
- Blender (RRID:SCR_008606) (data or information resource)
- Volocity 3D Image Analysis Software (RRID:SCR_002668) (software resource)
- Adobe Illustrator (RRID:SCR_010279) (software resource)
Additional research tools detected in this publication
None foundAntibodies used in this publication
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DO NOT USE THIS RRID. THIS IS NOT AN ANTIBODY.
Alexa Fluor 594 Phalloidin (RRID:AB_2315633) - Donkey Anti-Mouse IgG (H+L) Antibody, Alexa Fluor ?? 594 Conjugated (RRID:AB_141633)
- Donkey Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor ?? 594 Conjugated (RRID:AB_141637)
- Donkey Anti-Goat IgG (H+L) Antibody, Alexa Fluor ?? 594 Conjugated (RRID:AB_142540)
- Donkey Anti-Goat IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (RRID:AB_2534102)
- Synaptophysin Monoclonal Antibody (SP11) (RRID:AB_10983675)
- Rhodamine (TRITC)-AffiniPure Rabbit Anti-Horseradish Peroxidase (RRID:AB_2340257)
- Glutamate receptor subunit, DGluR-IIA antibody - Goodman, C.; University of California, Berkeley (RRID:AB_528269)
- Bruchpilot antibody - Buchner, E.; Universitaetsklinikim Wuerzburg (RRID:AB_2314866)
- Spectrin, alpha antibody - Branton, D. / Dubreuil, R.; Biology, Harvard University (RRID:AB_528473)
- Discs large (Drosophila) antibody - Goodman, C.; University of California, Berkeley (RRID:AB_528203)
- HA-probe Antibody (Y-11) (RRID:AB_631618)
- Anti-HA.11 Epitope Tag (RRID:AB_2565335)
- Rabbit Anti-Myosin IIA, non muscle Antibody, Unconjugated (RRID:AB_260673)
- Mouse Anti-Chicken Vinculin Monoclonal Antibody, Unconjugated, Clone VIN-11-5 (RRID:AB_477617)
- Mouse Anti-Arp2 Monoclonal Antibody, Unconjugated, Clone FMS96 (RRID:AB_867730)
- Cortactin (H-191) (RRID:AB_2088281)
- fish (M-300) (RRID:AB_2254551)
- Goat Anti-Dynamin II Polyclonal antibody, Unconjugated (RRID:AB_639943)
Associated grants
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This is a list of tools and resources that we have found mentioned in this publication.
DO NOT USE THIS RRID. THIS IS NOT AN ANTIBODY.
Alexa Fluor 594 Phalloidin (antibody)
RRID:AB_2315633
This unknown targets
View all literature mentionsDonkey Anti-Mouse IgG (H+L) Antibody, Alexa Fluor ?? 594 Conjugated (antibody)
RRID:AB_141633
This unknown targets Mouse IgG (H+L)
View all literature mentionsDonkey Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor ?? 594 Conjugated (antibody)
RRID:AB_141637
This unknown targets Rabbit IgG (H+L)
View all literature mentionsDonkey Anti-Goat IgG (H+L) Antibody, Alexa Fluor ?? 594 Conjugated (antibody)
RRID:AB_142540
This unknown targets Goat IgG (H+L)
View all literature mentionsDonkey Anti-Goat IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (antibody)
RRID:AB_2534102
This unknown targets IgG (H+L)
View all literature mentionsSynaptophysin Monoclonal Antibody (SP11) (antibody)
RRID:AB_10983675
This monoclonal targets Synaptophysin
View all literature mentionsRhodamine (TRITC)-AffiniPure Rabbit Anti-Horseradish Peroxidase (antibody)
RRID:AB_2340257
This unknown targets Horseradish Peroxidase
View all literature mentionsGlutamate receptor subunit, DGluR-IIA antibody - Goodman, C.; University of California, Berkeley (antibody)
RRID:AB_528269
This monoclonal targets Glutamate receptor subunit, DGluR-IIA
View all literature mentionsBruchpilot antibody - Buchner, E.; Universitaetsklinikim Wuerzburg (antibody)
RRID:AB_2314866
This monoclonal targets Bruchpilot
View all literature mentionsSpectrin, alpha antibody - Branton, D. / Dubreuil, R.; Biology, Harvard University (antibody)
RRID:AB_528473
This monoclonal targets Spectrin, alpha
View all literature mentionsDiscs large (Drosophila) antibody - Goodman, C.; University of California, Berkeley (antibody)
RRID:AB_528203
This monoclonal targets Discs large (Drosophila)
View all literature mentionsHA-probe Antibody (Y-11) (antibody)
RRID:AB_631618
This polyclonal targets HA-probe
View all literature mentionsAnti-HA.11 Epitope Tag (antibody)
RRID:AB_2565335
This monoclonal targets HA.11
View all literature mentionsRabbit Anti-Myosin IIA, non muscle Antibody, Unconjugated (antibody)
RRID:AB_260673
This unknown targets Myosin IIA, non muscle
View all literature mentionsMouse Anti-Chicken Vinculin Monoclonal Antibody, Unconjugated, Clone VIN-11-5 (antibody)
RRID:AB_477617
This monoclonal targets Vinculin
View all literature mentionsMouse Anti-Arp2 Monoclonal Antibody, Unconjugated, Clone FMS96 (antibody)
RRID:AB_867730
This monoclonal targets Arp2
View all literature mentionsCortactin (H-191) (antibody)
RRID:AB_2088281
This polyclonal targets CTTN
View all literature mentionsfish (M-300) (antibody)
RRID:AB_2254551
This polyclonal targets fish (M-300)
View all literature mentionsGoat Anti-Dynamin II Polyclonal antibody, Unconjugated (antibody)
RRID:AB_639943
This polyclonal targets DNM2
View all literature mentionsC2C12 (cell line)
RRID:CVCL_0188
Cell line C2C12 is a Spontaneously immortalized cell line with a species of origin Mus musculus (Mouse)
View all literature mentionspClamp (software resource)
RRID:SCR_011323
Software suite for electrophysiology data acquisition and analysis by Molecular Devices. Used for the control and recording of voltage clamp, current clamp, and patch clamp experiments. The software suite consists of Clampex 11 Software for data acquisition, AxoScope 11 Software for background recording, Clampfit 11 Software for data analysis, and optional Clampfit Advanced Analysis Module for sophisticated and streamlined analysis.
View all literature mentionsZEISS ZEN Microscopy Software (software resource)
RRID:SCR_013672
User interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.
View all literature mentionsGraphPad Prism (software resource)
RRID:SCR_002798
Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsImageJ (software resource)
RRID:SCR_003070
Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsMetaMorph Microscopy Automation and Image Analysis Software (software resource)
RRID:SCR_002368
Software tool for automated microscope acquisition, device control, and image analysis. Used for integrating dissimilar fluorescent microscope hardware and peripherals into a single custom workstation, while providing all the tools needed to perform analysis of acquired images. Offers user friendly application modules for analysis such as cell signaling, cell counting, and protein expression.
View all literature mentionsBlender (data or information resource)
RRID:SCR_008606
Blender is the free open source 3D content creation suite, available for all major operating systems under the GNU General Public License. Because of the overwhelming success of the first open movie project, Ton Roosendaal, the Blender Foundation''s chairman, has established the Blender Institute. This now is the permanent office and studio to more efficiently organize the Blender Foundation goals, but especially to coordinate and facilitate Open Projects related to 3D movies, games or visual effects.
View all literature mentionsVolocity 3D Image Analysis Software (software resource)
RRID:SCR_002668
3D image analysis software to visualize, analyze and validate 3D fluorescence images from a wide range of confocal microscopy, widefield and high content screening systems. It is fully integrated for a seamless user experience.
View all literature mentionsAdobe Illustrator (software resource)
RRID:SCR_010279
Vector graphics software to create digital graphics, illustrations, and typography for several types of media: print, web, interactive, video, and mobile.
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