CNS-Native Myeloid Cells Drive Immune Suppression in the Brain Metastatic Niche through Cxcl10.
Brain metastasis (br-met) develops in an immunologically unique br-met niche. Central nervous system-native myeloid cells (CNS-myeloids) and bone-marrow-derived myeloid cells (BMDMs) cooperatively regulate brain immunity. The phenotypic heterogeneity and specific roles of these myeloid subsets in shaping the br-met niche to regulate br-met outgrowth have not been fully revealed. Applying multimodal single-cell analyses, we elucidated a heterogeneous but spatially defined CNS-myeloid response during br-met outgrowth. We found Ccr2+ BMDMs minimally influenced br-met while CNS-myeloid promoted br-met outgrowth. Additionally, br-met-associated CNS-myeloid exhibited downregulation of Cx3cr1. Cx3cr1 knockout in CNS-myeloid increased br-met incidence, leading to an enriched interferon response signature and Cxcl10 upregulation. Significantly, neutralization of Cxcl10 reduced br-met, while rCxcl10 increased br-met and recruited VISTAHi PD-L1+ CNS-myeloid to br-met lesions. Inhibiting VISTA- and PD-L1-signaling relieved immune suppression and reduced br-met burden. Our results demonstrate that loss of Cx3cr1 in CNS-myeloid triggers a Cxcl10-mediated vicious cycle, cultivating a br-met-promoting, immune-suppressive niche.
Pubmed ID: 33113353 RIS Download
Research resources used in this publication
- B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J (RRID:IMSR_JAX:007906) (organism)
- B6.129(Cg)-Ccr2tm2.1Ifc/J (RRID:IMSR_JAX:017586) (organism)
- B6.129P2(Cg)-Cx3cr1tm1Litt/J (RRID:IMSR_JAX:005582) (organism)
- C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J (RRID:IMSR_JAX:007900) (organism)
- B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2)Litt/WganJ (RRID:IMSR_JAX:021160) (organism)
- C57BL/6J (RRID:IMSR_JAX:000664) (organism)
- SIM-A9 (RRID:CVCL_5I31) (cell line)
Additional research tools detected in this publication
Antibodies used in this publication
- Anti-Mouse CD11c (N418)-142Nd (RRID:AB_2814737)
- ImmPACT DAB (3,3'-diaminobenzidine) (RRID:AB_2336520)
- Fluoromount-G T (RRID:AB_2572296)
- RRID NOT VALID - THIS IS NOT AN ANTIBODY: DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) (RRID:AB_2629482)
- InVivoPlus anti-mouse PD-L1 (B7-H1) (RRID:AB_10949073)
- InVivoPlus anti-mouse VISTA (RRID:AB_2736990)
- APC anti-mouse CD183 (CXCR3) (RRID:AB_1088994)
- APC/Cyanine7 anti-mouse I-A/I-E (RRID:AB_1659252)
- APC/Cyanine7 anti-mouse NK-1.1 (RRID:AB_830870)
- PE/Dazzle(TM) 594 anti-mouse CD11c (RRID:AB_2563654)
- APC anti-mouse Ly-6G (RRID:AB_1877163)
- Alexa Fluor(R) 594 anti-mouse Ly-6G/Ly-6C (Gr-1) (RRID:AB_2563227)
- APC anti-mouse Ly-6C (RRID:AB_1732087)
- PE anti-mouse Ly-6C (RRID:AB_1186133)
- FITC anti-mouse Ly-6C (RRID:AB_1186134)
- APC anti-mouse F4/80 (RRID:AB_893481)
- APC/Cyanine7 anti-mouse/human CD11b (RRID:AB_830641)
- APC anti-mouse/human CD11b (RRID:AB_312794)
- APC/Cyanine7 anti-mouse CD8a (RRID:AB_312752)
- PE anti-mouse CD4 (RRID:AB_312692)
- Alexa Fluor(R) 647 anti-mouse CD3 (RRID:AB_389323)
- Brilliant Violet 421(TM) anti-mouse CD45 (RRID:AB_10899570)
- TotalSeq(TM)-A0308 anti-mouse Hashtag 8 (RRID:AB_2750040)
- TotalSeq(TM)-A0307 anti-mouse Hashtag 7 (RRID:AB_2750039)
- TotalSeq(TM)-A0306 anti-mouse Hashtag 6 (RRID:AB_2750037)
- TotalSeq(TM)-A0305 anti-mouse Hashtag 5 (RRID:AB_2750036)
- TotalSeq(TM)-A0304 anti-mouse Hashtag 4 (RRID:AB_2750035)
- TotalSeq(TM)-A0303 anti-mouse Hashtag 3 (RRID:AB_2750034)
- TotalSeq(TM)-A0302 anti-mouse Hashtag 2 (RRID:AB_2750033)
- TotalSeq(TM)-A0301 anti-mouse Hashtag 1 (RRID:AB_2750032)
- TotalSeq(TM)-A0078 anti-mouse CD49d (RRID:AB_2734159)
- TotalSeq(TM)-A0201 anti-mouse CD103 (RRID:AB_2750349)
- TotalSeq(TM)-A0212 anti-mouse CD24 (RRID:AB_2750380)
- TotalSeq(TM)-A0568 anti-mouse/rat XCR1 (RRID:AB_2783120)
- TotalSeq(TM)-A0445 anti-TMEM119 (Extracellular) (RRID:AB_2801201)
- TotalSeq(TM)-A0440 anti-mouse CD169 (Siglec-1) (RRID:AB_2783106)
- TotalSeq(TM)-A0190 anti-mouse CD274 (B7-H1, PD-L1) (RRID:AB_2783125)
- TotalSeq(TM)-A0004 anti-mouse CD279 (PD-1) (RRID:AB_2734169)
- TotalSeq(TM)-A0118 anti-mouse NK-1.1 (RRID:AB_2750536)
- TotalSeq(TM)-A0015 anti-mouse Ly-6G (RRID:AB_2749962)
- TotalSeq(TM)-A0013 anti-mouse Ly-6C (RRID:AB_2749961)
- TotalSeq(TM)-A0117 anti-mouse I-A/I-E (RRID:AB_2750505)
- TotalSeq(TM)-A0114 anti-mouse F4/80 (RRID:AB_2749986)
- TotalSeq(TM)-A0563 anti-mouse CX3CR1 (RRID:AB_2783121)
- TotalSeq(TM)-A0380 anti-rat CD90/mouse CD90.1 (Thy-1.1) (RRID:AB_2783141)
- TotalSeq(TM)-A0002 anti-mouse CD8a (RRID:AB_2734151)
- TotalSeq(TM)-A0200 anti-mouse CD86 (RRID:AB_2750348)
- TotalSeq(TM)-A0103 anti-mouse/human CD45R/B220 (RRID:AB_2734158)
- TotalSeq(TM)-A0096 anti-mouse CD45 (RRID:AB_2734156)
- TotalSeq(TM)-A0073 anti-mouse/human CD44 (RRID:AB_2734154)
- TotalSeq(TM)-A0001 anti-mouse CD4 (RRID:AB_2749956)
- TotalSeq(TM)-A0557 anti-mouse CD38 (RRID:AB_2750556)
- TotalSeq(TM)-A0182 anti-mouse CD3 (RRID:AB_2750533)
- TotalSeq(TM)-A0097 anti-mouse CD25 (RRID:AB_2749982)
- TotalSeq(TM)-A0422 anti-mouse CD172a (SIRPalpha) (RRID:AB_2800670)
- TotalSeq(TM)-A0106 anti-mouse CD11c (RRID:AB_2750352)
- TotalSeq(TM)-A0014 anti-mouse/human CD11b (RRID:AB_2734152)
- TotalSeq(TM)-A0012 anti-mouse CD117 (c-kit) (RRID:AB_2749960)
- TotalSeq(TM)-A0426 anti-mouse CD192 (CCR2) (RRID:AB_2783122)
- Anti-Mouse CD8a (53-6.7)-168Er (RRID:AB_2811241)
- Anti-Mouse CD117/c-kit (2B8)-166Er (RRID:AB_2801435)
- Anti-Mouse CX3CR1 (SA011F11)-164Dy (RRID:AB_2832247)
- Anti-Mouse CD279/PD-1 (29F.1A12)-159Tb Antibody (RRID:AB_2687839)
- Anti-Mouse CD274/PD-L1 (10F.9G2)-153Eu Antibody (RRID:AB_2687837)
- Anti-Mouse CD3e (145-2C11)-152Sm Antibody (RRID:AB_2687836)
- Anti- CD25 - 151Eu (RRID:AB_2827880)
- Anti- CD44 - 150Nd (RRID:AB_2827882)
- Anti-Mouse CD11b (M1/70)-148Nd (RRID:AB_2814738)
- Anti-Mouse CD4 (RM4-5)-145Nd Antibody (RRID:AB_2687832)
- Anti-Mouse CD45R/B220 (RA3-6B2)-144Nd (RRID:AB_2811239)
- Anti-Mouse Ly-6G (1A8)-141Pr (RRID:AB_2814678)
- Alexa Fluor 594-AffiniPure Goat Anti-Mouse IgG, Fc_ Fragment Specific (RRID:AB_2338873)
- Alexa Fluor 594-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) (min X Bov,Ck,Gt,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot) (RRID:AB_2340474)
- Donkey Anti-Goat IgG (H+L) Antibody, Alexa Fluor ?? 555 Conjugated (RRID:AB_141788)
- Alexa Fluor 488-AffiniPure Goat Anti-Rabbit IgG, Fc Fragment Specific (RRID:AB_2338048)
- Alexa Fluor 488-AffiniPure Donkey Anti-Rabbit IgG (H+L) (RRID:AB_2313584)
- Ki-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated) (RRID:AB_2620142)
- Anti Iba1, Rabbit antibody (RRID:AB_839504)
- VISTA (D5L5T) XP® Rabbit mAb (RRID:AB_2799474)
- Anti-TMEM119 antibody [28-3] - Microglial marker (RRID:AB_2800343)
- Anti-TMEM119 (RRID:AB_2744645)
- Living Colors® mCherry Monoclonal Antibody (RRID:AB_2307319)
- CD3 antibody [SP7] (RRID:AB_443425)
- TotalSeq(TM)-A0119 anti-mouse Siglec H (RRID:AB_2750537)
Associated grants
- Agency: NCI NIH HHS, United States
Id: R01 CA194697 - Agency: NCI NIH HHS, United States
Id: R01 CA222405
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This is a list of tools and resources that we have found mentioned in this publication.
Fiji (tool)
RRID:SCR_002285
Software package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsBio X Cell (tool)
RRID:SCR_004997
Commercial supplier and developer of in vivo antibodies. Provides antibodies and antibody production services.
View all literature mentionsVelocyto (tool)
RRID:SCR_018167
Software package for analysis of expression dynamics in single cell RNA seq data. Enables estimations of RNA velocities of single cells by distinguishing unspliced and spliced mRNAs in standard single-cell RNA sequencing protocols. Estimates RNA velocity in single cell RNA sequencing datasets. RNA velocity estimation in Python or R.
View all literature mentionsAnti-Mouse CD11c (N418)-142Nd (antibody)
RRID:AB_2814737
This monoclonal targets CD11c
View all literature mentionsImmPACT DAB (3,3'-diaminobenzidine) (antibody)
RRID:AB_2336520
This unknown targets
View all literature mentionsRRID NOT VALID - THIS IS NOT AN ANTIBODY: DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) (antibody)
RRID:AB_2629482
This unknown targets
View all literature mentionsInVivoPlus anti-mouse PD-L1 (B7-H1) (antibody)
RRID:AB_10949073
This monoclonal targets PD-L1 (B7-H1)
View all literature mentionsInVivoPlus anti-mouse VISTA (antibody)
RRID:AB_2736990
This monoclonal targets VISTA
View all literature mentionsAPC anti-mouse CD183 (CXCR3) (antibody)
RRID:AB_1088994
This monoclonal targets CD183
View all literature mentionsAPC/Cyanine7 anti-mouse I-A/I-E (antibody)
RRID:AB_1659252
This monoclonal targets I-A/I-E
View all literature mentionsAPC/Cyanine7 anti-mouse NK-1.1 (antibody)
RRID:AB_830870
This monoclonal targets NK-1.1
View all literature mentionsPE/Dazzle(TM) 594 anti-mouse CD11c (antibody)
RRID:AB_2563654
This monoclonal targets CD11c
View all literature mentionsAPC anti-mouse Ly-6G (antibody)
RRID:AB_1877163
This monoclonal targets Ly-6G
View all literature mentionsAlexa Fluor(R) 594 anti-mouse Ly-6G/Ly-6C (Gr-1) (antibody)
RRID:AB_2563227
This monoclonal targets Ly-6G Ly-6C
View all literature mentionsAPC anti-mouse Ly-6C (antibody)
RRID:AB_1732087
This monoclonal targets Ly-6C
View all literature mentionsPE anti-mouse Ly-6C (antibody)
RRID:AB_1186133
This monoclonal targets Ly-6C
View all literature mentionsFITC anti-mouse Ly-6C (antibody)
RRID:AB_1186134
This monoclonal targets Ly-6C
View all literature mentionsAPC anti-mouse F4/80 (antibody)
RRID:AB_893481
This monoclonal targets F4/80
View all literature mentionsAPC/Cyanine7 anti-mouse/human CD11b (antibody)
RRID:AB_830641
This monoclonal targets CD11b
View all literature mentionsAPC anti-mouse/human CD11b (antibody)
RRID:AB_312794
This monoclonal targets CD11b
View all literature mentionsAPC/Cyanine7 anti-mouse CD8a (antibody)
RRID:AB_312752
This monoclonal targets CD8a
View all literature mentionsPE anti-mouse CD4 (antibody)
RRID:AB_312692
This monoclonal targets CD4
View all literature mentionsAlexa Fluor(R) 647 anti-mouse CD3 (antibody)
RRID:AB_389323
This monoclonal targets CD3
View all literature mentionsBrilliant Violet 421(TM) anti-mouse CD45 (antibody)
RRID:AB_10899570
This monoclonal targets CD45
View all literature mentionsTotalSeq(TM)-A0308 anti-mouse Hashtag 8 (antibody)
RRID:AB_2750040
This monoclonal targets H-2/CD45
View all literature mentionsTotalSeq(TM)-A0307 anti-mouse Hashtag 7 (antibody)
RRID:AB_2750039
This monoclonal targets H-2/CD45
View all literature mentionsTotalSeq(TM)-A0306 anti-mouse Hashtag 6 (antibody)
RRID:AB_2750037
This monoclonal targets H-2/CD45
View all literature mentionsTotalSeq(TM)-A0305 anti-mouse Hashtag 5 (antibody)
RRID:AB_2750036
This monoclonal targets H-2/CD45
View all literature mentionsTotalSeq(TM)-A0304 anti-mouse Hashtag 4 (antibody)
RRID:AB_2750035
This monoclonal targets H-2/CD45
View all literature mentionsTotalSeq(TM)-A0303 anti-mouse Hashtag 3 (antibody)
RRID:AB_2750034
This monoclonal targets H-2/CD45
View all literature mentionsTotalSeq(TM)-A0302 anti-mouse Hashtag 2 (antibody)
RRID:AB_2750033
This monoclonal targets H-2/CD45
View all literature mentionsTotalSeq(TM)-A0301 anti-mouse Hashtag 1 (antibody)
RRID:AB_2750032
This monoclonal targets H-2/CD45
View all literature mentionsTotalSeq(TM)-A0078 anti-mouse CD49d (antibody)
RRID:AB_2734159
This monoclonal targets CD49d
View all literature mentionsTotalSeq(TM)-A0201 anti-mouse CD103 (antibody)
RRID:AB_2750349
This monoclonal targets CD103
View all literature mentionsTotalSeq(TM)-A0212 anti-mouse CD24 (antibody)
RRID:AB_2750380
This monoclonal targets CD24
View all literature mentionsTotalSeq(TM)-A0568 anti-mouse/rat XCR1 (antibody)
RRID:AB_2783120
This monoclonal targets XCR1
View all literature mentionsTotalSeq(TM)-A0445 anti-TMEM119 (Extracellular) (antibody)
RRID:AB_2801201
This monoclonal targets TMEM119
View all literature mentionsTotalSeq(TM)-A0440 anti-mouse CD169 (Siglec-1) (antibody)
RRID:AB_2783106
This monoclonal targets CD169
View all literature mentionsTotalSeq(TM)-A0190 anti-mouse CD274 (B7-H1, PD-L1) (antibody)
RRID:AB_2783125
This monoclonal targets CD274
View all literature mentionsTotalSeq(TM)-A0004 anti-mouse CD279 (PD-1) (antibody)
RRID:AB_2734169
This monoclonal targets CD279
View all literature mentionsTotalSeq(TM)-A0118 anti-mouse NK-1.1 (antibody)
RRID:AB_2750536
This monoclonal targets NK-1.1
View all literature mentionsTotalSeq(TM)-A0015 anti-mouse Ly-6G (antibody)
RRID:AB_2749962
This monoclonal targets Ly-6G
View all literature mentionsTotalSeq(TM)-A0013 anti-mouse Ly-6C (antibody)
RRID:AB_2749961
This monoclonal targets Ly-6C
View all literature mentionsTotalSeq(TM)-A0117 anti-mouse I-A/I-E (antibody)
RRID:AB_2750505
This monoclonal targets I-A/I-E
View all literature mentionsTotalSeq(TM)-A0114 anti-mouse F4/80 (antibody)
RRID:AB_2749986
This monoclonal targets F4/80
View all literature mentionsTotalSeq(TM)-A0563 anti-mouse CX3CR1 (antibody)
RRID:AB_2783121
This monoclonal targets CX3CR1
View all literature mentionsTotalSeq(TM)-A0380 anti-rat CD90/mouse CD90.1 (Thy-1.1) (antibody)
RRID:AB_2783141
This monoclonal targets CD90/CD90.1
View all literature mentionsTotalSeq(TM)-A0002 anti-mouse CD8a (antibody)
RRID:AB_2734151
This monoclonal targets CD8a
View all literature mentionsTotalSeq(TM)-A0200 anti-mouse CD86 (antibody)
RRID:AB_2750348
This monoclonal targets CD86
View all literature mentionsTotalSeq(TM)-A0103 anti-mouse/human CD45R/B220 (antibody)
RRID:AB_2734158
This monoclonal targets CD45R
View all literature mentionsTotalSeq(TM)-A0096 anti-mouse CD45 (antibody)
RRID:AB_2734156
This monoclonal targets CD45
View all literature mentionsTotalSeq(TM)-A0073 anti-mouse/human CD44 (antibody)
RRID:AB_2734154
This monoclonal targets CD44
View all literature mentionsTotalSeq(TM)-A0001 anti-mouse CD4 (antibody)
RRID:AB_2749956
This monoclonal targets CD4
View all literature mentionsTotalSeq(TM)-A0557 anti-mouse CD38 (antibody)
RRID:AB_2750556
This monoclonal targets CD38
View all literature mentionsTotalSeq(TM)-A0182 anti-mouse CD3 (antibody)
RRID:AB_2750533
This monoclonal targets CD3
View all literature mentionsTotalSeq(TM)-A0097 anti-mouse CD25 (antibody)
RRID:AB_2749982
This monoclonal targets CD25
View all literature mentionsTotalSeq(TM)-A0422 anti-mouse CD172a (SIRPalpha) (antibody)
RRID:AB_2800670
This monoclonal targets CD172a
View all literature mentionsTotalSeq(TM)-A0106 anti-mouse CD11c (antibody)
RRID:AB_2750352
This monoclonal targets CD11c
View all literature mentionsTotalSeq(TM)-A0014 anti-mouse/human CD11b (antibody)
RRID:AB_2734152
This monoclonal targets CD11b
View all literature mentionsTotalSeq(TM)-A0012 anti-mouse CD117 (c-kit) (antibody)
RRID:AB_2749960
This monoclonal targets CD117
View all literature mentionsTotalSeq(TM)-A0426 anti-mouse CD192 (CCR2) (antibody)
RRID:AB_2783122
This monoclonal targets CD192
View all literature mentionsAnti-Mouse CD8a (53-6.7)-168Er (antibody)
RRID:AB_2811241
This monoclonal targets CD8a
View all literature mentionsAnti-Mouse CD117/c-kit (2B8)-166Er (antibody)
RRID:AB_2801435
This monoclonal targets CD117/c-kit
View all literature mentionsAnti-Mouse CX3CR1 (SA011F11)-164Dy (antibody)
RRID:AB_2832247
This monoclonal targets CX3CR1
View all literature mentionsAnti-Mouse CD279/PD-1 (29F.1A12)-159Tb Antibody (antibody)
RRID:AB_2687839
This monoclonal targets CD279/PD-1
View all literature mentionsAnti-Mouse CD274/PD-L1 (10F.9G2)-153Eu Antibody (antibody)
RRID:AB_2687837
This monoclonal targets CD274/PD-L1
View all literature mentionsAnti-Mouse CD3e (145-2C11)-152Sm Antibody (antibody)
RRID:AB_2687836
This monoclonal targets CD3e
View all literature mentionsAnti- CD25 - 151Eu (antibody)
RRID:AB_2827880
This monoclonal targets CD25
View all literature mentionsAnti- CD44 - 150Nd (antibody)
RRID:AB_2827882
This monoclonal targets CD44
View all literature mentionsAnti-Mouse CD11b (M1/70)-148Nd (antibody)
RRID:AB_2814738
This monoclonal targets CD11b/Mac-1
View all literature mentionsAnti-Mouse CD4 (RM4-5)-145Nd Antibody (antibody)
RRID:AB_2687832
This monoclonal targets CD4
View all literature mentionsAnti-Mouse CD45R/B220 (RA3-6B2)-144Nd (antibody)
RRID:AB_2811239
This monoclonal targets CD45R/B220
View all literature mentionsAnti-Mouse Ly-6G (1A8)-141Pr (antibody)
RRID:AB_2814678
This monoclonal targets Ly-6G
View all literature mentionsAlexa Fluor 594-AffiniPure Goat Anti-Mouse IgG, Fc_ Fragment Specific (antibody)
RRID:AB_2338873
This unknown targets Mouse IgG, Fc? Fragment Specific
View all literature mentionsAlexa Fluor 594-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) (min X Bov,Ck,Gt,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot) (antibody)
RRID:AB_2340474
This unknown targets Guinea Pig IgG (H+L)
View all literature mentionsDonkey Anti-Goat IgG (H+L) Antibody, Alexa Fluor ?? 555 Conjugated (antibody)
RRID:AB_141788
This unknown targets Goat IgG (H+L)
View all literature mentionsAlexa Fluor 488-AffiniPure Goat Anti-Rabbit IgG, Fc Fragment Specific (antibody)
RRID:AB_2338048
This unknown targets Rabbit IgG, Fc Fragment Specific
View all literature mentionsAlexa Fluor 488-AffiniPure Donkey Anti-Rabbit IgG (H+L) (antibody)
RRID:AB_2313584
This polyclonal secondary targets IgG (H+L)
View all literature mentionsKi-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated) (antibody)
RRID:AB_2620142
This monoclonal targets Ki-67
View all literature mentionsAnti Iba1, Rabbit antibody (antibody)
RRID:AB_839504
This polyclonal targets Iba1
View all literature mentionsVISTA (D5L5T) XP® Rabbit mAb (antibody)
RRID:AB_2799474
This monoclonal targets VISTA
View all literature mentionsAnti-TMEM119 antibody [28-3] - Microglial marker (antibody)
RRID:AB_2800343
This monoclonal targets TMEM119
View all literature mentionsAnti-TMEM119 (antibody)
RRID:AB_2744645
This polyclonal targets TMEM119
View all literature mentionsLiving Colors® mCherry Monoclonal Antibody (antibody)
RRID:AB_2307319
This monoclonal targets mCherry
View all literature mentionsCD3 antibody [SP7] (antibody)
RRID:AB_443425
This monoclonal targets CD3 antibody [SP7]
View all literature mentionsTotalSeq(TM)-A0119 anti-mouse Siglec H (antibody)
RRID:AB_2750537
This monoclonal targets Siglec H
View all literature mentionsB6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J (organism)
RRID:IMSR_JAX:007906
Mus musculus with name B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J from IMSR.
View all literature mentionsB6.129(Cg)-Ccr2tm2.1Ifc/J (organism)
RRID:IMSR_JAX:017586
Mus musculus with name B6.129(Cg)-Ccr2tm2.1Ifc/J from IMSR.
View all literature mentionsB6.129P2(Cg)-Cx3cr1tm1Litt/J (organism)
RRID:IMSR_JAX:005582
Mus musculus with name B6.129P2(Cg)-Cx3cr1tm1Litt/J from IMSR.
View all literature mentionsC57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J (organism)
RRID:IMSR_JAX:007900
Mus musculus with name C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J from IMSR.
View all literature mentionsB6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2)Litt/WganJ (organism)
RRID:IMSR_JAX:021160
Mus musculus with name B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2)Litt/WganJ from IMSR.
View all literature mentionsC57BL/6J (organism)
RRID:IMSR_JAX:000664
Mus musculus with name C57BL/6J from IMSR.
View all literature mentionsSIM-A9 (cell line)
RRID:CVCL_5I31
Cell line SIM-A9 is a Spontaneously immortalized cell line with a species of origin Mus musculus
View all literature mentions
