Intermediate progenitors support migration of neural stem cells into dentate gyrus outer neurogenic niches.
The hippocampal dentate gyrus (DG) is a unique brain region maintaining neural stem cells (NCSs) and neurogenesis into adulthood. We used multiphoton imaging to visualize genetically defined progenitor subpopulations in live slices across key stages of mouse DG development, testing decades old static models of DG formation with molecular identification, genetic-lineage tracing, and mutant analyses. We found novel progenitor migrations, timings, dynamic cell-cell interactions, signaling activities, and routes underlie mosaic DG formation. Intermediate progenitors (IPs, Tbr2+) pioneered migrations, supporting and guiding later emigrating NSCs (Sox9+) through multiple transient zones prior to converging at the nascent outer adult niche in a dynamic settling process, generating all prenatal and postnatal granule neurons in defined spatiotemporal order. IPs (Dll1+) extensively targeted contacts to mitotic NSCs (Notch active), revealing a substrate for cell-cell contact support during migrations, a developmental feature maintained in adults. Mouse DG formation shares conserved features of human neocortical expansion.
Pubmed ID: 32238264 RIS Download
Research resources used in this publication
None foundAdditional research tools detected in this publication
Antibodies used in this publication
- Anti-GFP antibody (RRID:AB_300798)
- Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein (Dako Omnis) (RRID:AB_2811722)
- EOMES Monoclonal Antibody (Dan11mag), eBioscience (RRID:AB_11042577)
- EOMES Monoclonal Antibody (WD1928), eBioscience (RRID:AB_2572881)
- Anti-Prospero homeobox protein 1/PROX1 Antibody (RRID:AB_2811075)
- Mouse Anti-PCNA (Proliferating Cell Nuclear Antigen) Monoclonal antibody, Unconjugated, Clone 19f4 (RRID:AB_95029)
- Anti-Sox9 (RRID:AB_2239761)
Associated grants
- Agency: NICHD NIH HHS, United States
Id: P50 HD103524 - Agency: NINDS NIH HHS, United States
Id: R01 NS085081 - Agency: NIMH NIH HHS, United States
Id: R21 MH087070 - Agency: NINDS NIH HHS, United States
Id: R01 NS092339 - Agency: NINDS NIH HHS, United States
Id: R01 NS099027 - Agency: NIH HHS, United States
Id: R21 OD023838
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Covance (tool)
RRID:SCR_001224
A contract research organization providing drug development and animal testing services. Under the name Covance Research Products Inc., based in Denver, Pennsylvania, the company also deals in the import, breeding and sale of laboratory animals. It breeds dogs, rabbits, guinea pigs, non-human primates, and pigs, and runs the largest non-human primate laboratory in Germany. (Wikipedia)
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RRID:SCR_013530
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View all literature mentionsAnti-GFP antibody (antibody)
RRID:AB_300798
This polyclonal targets GFP
View all literature mentionsPolyclonal Rabbit Anti-Glial Fibrillary Acidic Protein (Dako Omnis) (antibody)
RRID:AB_2811722
This polyclonal targets GFAP
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This monoclonal targets EOMES
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RRID:AB_95029
This monoclonal targets PCNA (Proliferating Cell Nuclear Antigen)
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