X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

p16 deficiency attenuates intervertebral disc degeneration by adjusting oxidative stress and nucleus pulposus cell cycle.

Hui Che | Jie Li | You Li | Cheng Ma | Huan Liu | Jingyi Qin | Jianghui Dong | Zhen Zhang | Cory J Xian | Dengshun Miao | Liping Wang | Yongxin Ren
eLife | 2020

The cell cycle regulator p16 is known as a biomarker and an effector of aging. However, its function in intervertebral disc degeneration (IVDD) is unclear. In this study, p16 expression levels were found to be positively correlated with the severity of human IVDD. In a mouse tail suspension (TS)-induced IVDD model, lumbar intervertebral disc height index and matrix protein expression levels were reduced significantly were largely rescued by p16 deletion. In TS mouse discs, reactive oxygen species levels, proportions of senescent cells, and the senescence-associated secretory phenotype (SASP) were all increased, cell cycling was delayed, and expression was downregulated for Sirt1, superoxide dismutase 1/2, cyclin-dependent kinases 4/6, phosphorylated retinoblastoma protein, and transcription factor E2F1/2. However, these effects were rescued by p16 deletion. Our results demonstrate that p16 plays an important role in IVDD pathogenesis and that its deletion attenuates IVDD by promoting cell cycle and inhibiting SASP, cell senescence, and oxidative stress.

Pubmed ID: 32125276 RIS Download

Associated grants

None

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


Addgene (tool)

RRID:SCR_002037

Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.

View all literature mentions

JASPAR (tool)

RRID:SCR_003030

Open source database of curated, non-redundant set of profiles derived from published collections of experimentally defined transcription factor binding sites for multicellular eukaryotes. Consists of open data access, non-redundancy and quality. JASPAR CORE is smaller set that is non-redundant and curated. Collection of transcription factor DNA-binding preferences, modeled as matrices. These can be converted into Position Weight Matrices (PWMs or PSSMs), used for scanning genomic sequences. Web interface for browsing, searching and subset selection, online sequence analysis utility and suite of programming tools for genome-wide and comparative genomic analysis of regulatory regions. New functions include clustering of matrix models by similarity, generation of random matrices by sampling from selected sets of existing models and a language-independent Web Service applications programming interface for matrix retrieval.

View all literature mentions

NCBI (tool)

RRID:SCR_006472

A portal to biomedical and genomic information. NCBI creates public databases, conducts research in computational biology, develops software tools for analyzing genome data, and disseminates biomedical information for the better understanding of molecular processes affecting human health and disease.

View all literature mentions

Thermo Fisher Scientific (tool)

RRID:SCR_008452

Commercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.

View all literature mentions

Scion Image (tool)

RRID:SCR_008673

THIS RESOURCE IS NO LONGER IN SERVICE, documented on July 17, 2013. Commercial software vendor.

View all literature mentions

Abcam (tool)

RRID:SCR_012931

A commercial antibody supplier which supplies primary and secondary antibodies, biochemicals, proteins, peptides, lysates, immunoassays and other kits.

View all literature mentions

C57BL/6J (tool)

RRID:IMSR_JAX:000664

Mus musculus with name C57BL/6J from IMSR.

View all literature mentions

Ki67 antibody - Proliferation Marker (antibody)

RRID:AB_443209

This polyclonal targets Ki67 antibody - Proliferation Marker

View all literature mentions

TP53-human (antibody)

RRID:AB_628082

This monoclonal targets TP53

View all literature mentions

p16 ARC antibody [EP1551Y] (antibody)

RRID:AB_2059963

This monoclonal targets p16 ARC

View all literature mentions

Cdk6 (phospho Y24) antibody (antibody)

RRID:AB_11156738

This polyclonal targets Cdk6 (phospho Y24) antibody

View all literature mentions

VEGF 165A antibody [6B7] (antibody)

RRID:AB_1271452

This monoclonal targets VEGF 165A antibody [6B7]

View all literature mentions

NFkappaB p65 (4H211) (antibody)

RRID:AB_1126640

This monoclonal targets NFkappaB p65 (4H211)

View all literature mentions

IGF1 antibody (antibody)

RRID:AB_308724

This polyclonal targets IGF1

View all literature mentions

E2F2-human (antibody)

RRID:AB_2096793

This polyclonal targets E2F2

View all literature mentions

Rabbit Anti-Human MMP13 Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_776416

This polyclonal targets Human MMP13 - Hinge region

View all literature mentions

p-Rb (Tyr356) (7F10) (antibody)

RRID:AB_785453

This monoclonal targets p-Rb (Tyr356) (7F10)

View all literature mentions

Rabbit Anti-MnSOD Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_300434

This polyclonal targets Superoxide Dismutase 2

View all literature mentions

Superoxide Dismutase 1 antibody (antibody)

RRID:AB_300402

This polyclonal targets Superoxide Dismutase 1 antibody

View all literature mentions

Anti-SIRT1 antibody [19A7AB4] (antibody)

RRID:AB_10864359

This monoclonal targets SIRT1

View all literature mentions

PCNA antibody [EPR3821] (antibody)

RRID:AB_10561973

This monoclonal targets PCNA antibody [EPR3821]

View all literature mentions

GraphPad Prism (software resource)

RRID:SCR_002798

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

View all literature mentions

SPSS (software resource)

RRID:SCR_002865

Software package used for interactive, or batched, statistical analysis in social science, health sciences and marketing. Software platform offers advanced statistical analysis, a library of machine-learning algorithms, text analysis, open-source extensibility, integration with big data and deployment into applications.Versions that were produced by SPSS Inc. before the IBM acquisition (Versions 18 and earlier) would be given origin or publisher of SPSS Inc. in Chicago.

View all literature mentions