RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer. Here we investigated the molecular and phenotypic role of RUVBL1/2 ATPase activity in non-small cell lung cancer (NSCLC). We find that RUVBL1/2 are overexpressed in NSCLC patient tumors, with high expression associated with poor survival. Utilizing a specific inhibitor of RUVBL1/2 ATPase activity, we show that RUVBL1/2 ATPase activity is necessary for the maturation or dissociation of the PAQosome, a large RUVBL1/2-dependent multiprotein complex. We also show that RUVBL1/2 have roles in DNA replication, as inhibition of its ATPase activity can cause S-phase arrest, which culminates in cancer cell death via replication catastrophe. While in vivo pharmacological inhibition of RUVBL1/2 results in modest antitumor activity, it synergizes with radiation in NSCLC, but not normal cells, an attractive property for future preclinical development.
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This polyclonal targets RPA14 antibody
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsThis polyclonal targets RFC4 antibody
View all literature mentionsThis unknown targets POLE2
View all literature mentionsThis monoclonal targets PCNA (PC10)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets ATR
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis monoclonal targets ATM
View all literature mentionsThis monoclonal targets Human RPA70
View all literature mentionsThis polyclonal targets Human DNA PKcs (phospho S2056)
View all literature mentionsThis polyclonal targets PDRG1
View all literature mentionsThis polyclonal targets TELO2
View all literature mentionsThis unknown targets PFDN6
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets 53BP1
View all literature mentionsThis monoclonal targets H2AX
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsThis monoclonal targets ATM antibody [Y170]
View all literature mentionsThis recombinant monoclonal targets GAPDH
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentionsThis monoclonal targets RPA32/RPA2
View all literature mentionsThis monoclonal targets PARP
View all literature mentionsThis monoclonal targets alpha-Tubulin (DM1A) Mouse mAb
View all literature mentionsThis monoclonal targets phospho-Histone H2A.X (Ser139) clone JBW301 biotin conjugate
View all literature mentionsThis monoclonal targets Mouse (G3A1) mAb IgG1 Isotype Control
View all literature mentionsThis polyclonal targets V5 Tag
View all literature mentionsThis monoclonal targets HSP90AA1, HSP90AB1, HSP90B1
View all literature mentionsThis monoclonal targets BrdU (IIB5)
View all literature mentionsThis monoclonal targets Chk1 (G-4)
View all literature mentionsThis monoclonal targets V5 Tag
View all literature mentionsThis polyclonal targets Histone H3 (phospho S10) antibody - Mitosis Marker
View all literature mentionsThis polyclonal targets Phospho-Chk1 (Ser317)
View all literature mentionsThis monoclonal targets Histone H2A.X antibody [EPR895]
View all literature mentionsThis polyclonal targets PRKDC
View all literature mentionsThis polyclonal targets RUVBL2
View all literature mentionsMus musculus with name BALB/cByJ from IMSR.
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on September 28,2023. Software application which can analyze basic Western blot data and export them for use in spreadsheet programs. Users can create standardized or custom lab reports and can share them with colleagues. Image Studio Lite has been discontinued and replaced with Empiria Studio Software.
View all literature mentionsUser interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.
View all literature mentionsA web-based gene list enrichment analysis tool that provides various types of visualization summaries of collective functions of gene lists. It includes new gene-set libraries, an alternative approach to rank enriched terms, and various interactive visualization approaches to display enrichment results using the JavaScript library, Data Driven Documents (D3). The software can also be embedded into any tool that performs gene list analysis. System-wide profiling of genes and proteins in mammalian cells produce lists of differentially expressed genes / proteins that need to be further analyzed for their collective functions in order to extract new knowledge. Once unbiased lists of genes or proteins are generated from such experiments, these lists are used as input for computing enrichment with existing lists created from prior knowledge organized into gene-set libraries.
View all literature mentionsA a configurable software package for peptide and protein mass spectrometry analyses. It includes the SEQUEST search algorithm to identify separate proteins in complex mixtures, interactive navigation tools to filter and sort protein summaries, customized spectral plots, and chromatograms using the PEPMATCH and PEPMAP tools. This software also has batch processing capabilities to improve throughput by queuing up several files, and custom-build proprietary databases, index databases, and retrieve databases through a public server.
View all literature mentionsSoftware providing a data analysis pipeline for shotgun mass-spectrometry proteomics.
View all literature mentionsSoftware for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsSoftware package for interpreting gene expression data. Used for interpretation of a large-scale experiment by identifying pathways and processes.
View all literature mentionsMus musculus with name NOD.Cg-Prkdcscid/J from IMSR.
View all literature mentionsCell line DU145 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line Ramos.2G6.4C10 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line AsPC-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line IMR-90 is a Finite cell line with a species of origin Homo sapiens
View all literature mentionsCell line Calu-3 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HOP-62 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line A-549 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line Calu-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line Calu-6 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line PC-3 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SK-LU-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line PANC-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MIA PaCa-2 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line OVCAR-5 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsSoftware tool to enable biologists without training in computer vision or programming to quantitatively measure phenotypes from thousands of images automatically. It counts cells and also measures the size, shape, intensity and texture of every cell (and every labeled subcellular compartment) in every image. It was designed for high throughput screening but can perform automated image analysis for images from time-lapse movies and low-throughput experiments. CellProfiler has an increasing number of algorithms to identify and measure properties of neuronal cell types.
View all literature mentionsCell line NCI-H1993 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on September 28,2023. Software application which can analyze basic Western blot data and export them for use in spreadsheet programs. Users can create standardized or custom lab reports and can share them with colleagues. Image Studio Lite has been discontinued and replaced with Empiria Studio Software.
View all literature mentionsCell line A-427 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line DFCI024 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC827 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC193 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC78 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC44 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC2814 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H661 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H358 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H3122 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H2882 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H2347 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H2126 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H2087 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H2085 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H2030 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1975 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1944 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1755 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1693 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1581 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1437 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1355 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1048 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1792 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1719 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1373 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H596 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1195 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1650 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H157 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1666 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H2073 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1299 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1155 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1819 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC4019 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC95 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC15 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H226 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H810 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1833 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H460 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1648 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H522 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC4017 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H727 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H1703 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H838 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H820 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HBEC30-KT is a Telomerase immortalized cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HBEC3-KT is a Telomerase immortalized cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H2009 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NCI-H647 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentions