Self-Organization of Mouse Stem Cells into an Extended Potential Blastoid.
Mammalian blastocysts comprise three distinct cell lineages essential for development beyond implantation: the pluripotent epiblast, which generates the future embryo, and surrounding it the extra-embryonic primitive endoderm and the trophectoderm tissues. Embryonic stem cells can reintegrate into embryogenesis but contribute primarily to epiblast lineages. Here, we show that mouse embryonic stem cells cultured under extended pluripotent conditions (EPSCs) can be partnered with trophoblast stem cells to self-organize into blastocyst-like structures with all three embryonic and extra-embryonic lineages. Morphogenetic and transcriptome profiling analyses reveal that these blastocyst-like structures show distinct embryonic-abembryonic axes and primitive endoderm differentiation and can initiate the transition from the pre- to post-implantation egg cylinder morphology in vitro.
Pubmed ID: 31846649 RIS Download
Research resources used in this publication
None foundAdditional research tools detected in this publication
Antibodies used in this publication
- ZO-1 Monoclonal Antibody (ZO1-1A12) (RRID:AB_2533147)
- Mouse Anti-Cytokeratin 8 Monoclonal Antibody, Unconjugated, Clone C-04 (RRID:AB_305647)
- Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated (RRID:AB_2313606)
- Anti-Mouse IgG (H+L), made in goat (RRID:AB_2336171)
- Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (RRID:AB_2536183)
- Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (RRID:AB_2535864)
- Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (RRID:AB_2534013)
- Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2535794)
- Rabbit Anti-Mouse TBR2 / Eomes Polyclonal Antibody, Unconjugated (RRID:AB_778267)
- Proliferin (E-10) (RRID:AB_10710396)
- Goat Anti-Human Sox17 Polyclonal antibody, Unconjugated (RRID:AB_355060)
- Mouse Podocalyxin MAb (Clone 192703) (RRID:AB_2166010)
- PKC zeta (H-1) (RRID:AB_628148)
- Nanog antibody (RRID:AB_2150114)
- Oct3/4 Antibody (C-10) (RRID:AB_628051)
- Anti-Laminin antibody produced in rabbit (RRID:AB_477163)
- Ki-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated) (RRID:AB_2620142)
- Goat Anti-Human Gata-6 Polyclonal antibody, Unconjugated (RRID:AB_2108901)
- Anti-GFP (RRID:AB_10013361)
- GATA4-mouse (RRID:AB_2108747)
- E-cadherin Monoclonal Antibody (ECCD-2) (RRID:AB_2533005)
- COX2 / Cyclooxygenase 2 antibody (RRID:AB_2085144)
- FoxA2/HNF3 (D56D6) XP Rabbit mAb (RRID:AB_10891055)
- Goat Anti-Human AP-2 gamma Affinity Purified Polyclonal Antibody, Unconjugated (RRID:AB_2255891)
- CDX 2, caudal type homeo box transcription factor 2 (RRID:AB_2335627)
- Collagen IV antibody (RRID:AB_445160)
Associated grants
- Agency: European Research Council, International
Id: 669198 - Agency: NICHD NIH HHS, United States
Id: DP1 HD104575 - Agency: Wellcome Trust, United Kingdom
Id: 098287/Z/12/Z - Agency: Biotechnology and Biological Sciences Research Council, United Kingdom
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This is a list of tools and resources that we have found mentioned in this publication.
Adobe Illustrator (tool)
RRID:SCR_010279
Vector graphics software to create digital graphics, illustrations, and typography for several types of media: print, web, interactive, video, and mobile.
View all literature mentionsZO-1 Monoclonal Antibody (ZO1-1A12) (antibody)
RRID:AB_2533147
This monoclonal targets ZO-1
View all literature mentionsMouse Anti-Cytokeratin 8 Monoclonal Antibody, Unconjugated, Clone C-04 (antibody)
RRID:AB_305647
This monoclonal targets Cytokeratin 18
View all literature mentionsGoat Anti-Rabbit IgG Antibody (H+L), Biotinylated (antibody)
RRID:AB_2313606
This polyclonal targets IgG
View all literature mentionsAnti-Mouse IgG (H+L), made in goat (antibody)
RRID:AB_2336171
This unknown targets IgG
View all literature mentionsDonkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (antibody)
RRID:AB_2536183
This polyclonal secondary targets IgG (H+L)
View all literature mentionsDonkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (antibody)
RRID:AB_2535864
This polyclonal secondary targets IgG (H+L)
View all literature mentionsDonkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (antibody)
RRID:AB_2534013
This polyclonal secondary targets IgG (H+L)
View all literature mentionsDonkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_2535794
This polyclonal secondary targets IgG (H+L)
View all literature mentionsRabbit Anti-Mouse TBR2 / Eomes Polyclonal Antibody, Unconjugated (antibody)
RRID:AB_778267
This polyclonal targets Mouse TBR2 / Eomes
View all literature mentionsProliferin (E-10) (antibody)
RRID:AB_10710396
This monoclonal targets Proliferin (E-10)
View all literature mentionsGoat Anti-Human Sox17 Polyclonal antibody, Unconjugated (antibody)
RRID:AB_355060
This polyclonal targets Human SOX17
View all literature mentionsMouse Podocalyxin MAb (Clone 192703) (antibody)
RRID:AB_2166010
This monoclonal targets Mouse Podocalyxin MAb (Clone 192703)
View all literature mentionsNanog antibody (antibody)
RRID:AB_2150114
This polyclonal targets Nanog
View all literature mentionsOct3/4 Antibody (C-10) (antibody)
RRID:AB_628051
This monoclonal targets Oct-3/4
View all literature mentionsAnti-Laminin antibody produced in rabbit (antibody)
RRID:AB_477163
This polyclonal targets Laminin antibody produced in rabbit
View all literature mentionsKi-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated) (antibody)
RRID:AB_2620142
This monoclonal targets Ki-67
View all literature mentionsGoat Anti-Human Gata-6 Polyclonal antibody, Unconjugated (antibody)
RRID:AB_2108901
This polyclonal targets Human GATA-6
View all literature mentionsE-cadherin Monoclonal Antibody (ECCD-2) (antibody)
RRID:AB_2533005
This monoclonal targets E-cadherin
View all literature mentionsCOX2 / Cyclooxygenase 2 antibody (antibody)
RRID:AB_2085144
This polyclonal targets Ptgs2
View all literature mentionsFoxA2/HNF3 (D56D6) XP Rabbit mAb (antibody)
RRID:AB_10891055
This monoclonal targets FoxA2/HNF3
View all literature mentionsGoat Anti-Human AP-2 gamma Affinity Purified Polyclonal Antibody, Unconjugated (antibody)
RRID:AB_2255891
This polyclonal targets Human AP-2 gamma
View all literature mentionsCDX 2, caudal type homeo box transcription factor 2 (antibody)
RRID:AB_2335627
This monoclonal targets Raised against full length recombinant protein
View all literature mentionsCollagen IV antibody (antibody)
RRID:AB_445160
This polyclonal targets Collagen IV antibody
View all literature mentions
