Addgene (tool)

RRID:SCR_002037

Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.

PRISM (tool)

RRID:SCR_005375

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on May 5,2022.Tool that predicts interactions between transcription factors and their regulated genes from binding motifs. Understanding vertebrate development requires unraveling the cis-regulatory architecture of gene regulation. PRISM provides accurate genome-wide computational predictions of transcription factor binding sites for the human and mouse genomes, and integrates the predictions with GREAT to provide functional biological context. Together, accurate computational binding site prediction and GREAT produce for each transcription factor: 1. putative binding sites, 2. putative target genes, 3. putative biological roles of the transcription factor, and 4. putative cis-regulatory elements through which the factor regulates each target in each functional role.

QIAGEN (tool)

RRID:SCR_008539

A commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.

Millipore (tool)

RRID:SCR_008983

An Antibody supplier

Sigma-Aldrich (tool)

RRID:SCR_008988

American chemical, life science and biotechnology company owned by Merck KGaA. Merger of Sigma Chemical Company and Aldrich Chemical Company. Provides organic and inorganic chemicals, building blocks, reagents, advanced materials and stable isotopes for chemical synthesis, medicinal chemistry and materials science, antibiotics, buffers, carbohydrates, enzymes, forensic tools, hematology and histology, nucleotides, proteins, peptides, amino acids and their derivatives.

Abcam (tool)

RRID:SCR_012931

A commercial antibody supplier which supplies primary and secondary antibodies, biochemicals, proteins, peptides, lysates, immunoassays and other kits.

Agilent Technologies (tool)

RRID:SCR_013575

Company provides laboratories worldwide with analytical instruments and supplies, clinical and diagnostic testing services, consumables, applications and expertise in life sciences and applied chemical markets.

Rabbit IgG, polyclonal - Isotype Control (antibody)

RRID:AB_2631996

This polyclonal targets

Goat Anti-Human VDAC2 Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_778790

This polyclonal targets Human VDAC2

Anti-GFP antibody [9F9.F9] (antibody)

RRID:AB_298911

This monoclonal targets GFP

Anti-RAB7 antibody [EPR7589] (ab137029) (antibody)

RRID:AB_2629474

This monoclonal targets RAB7

Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)

RRID:AB_2534069

This polyclonal secondary targets IgG (H+L)

Mouse Anti-Rab 5 Monoclonal antibody, Unconjugated, Clone d-11 (antibody)

RRID:AB_628191

This monoclonal targets RAB5A

RPS3A antibody (antibody)

RRID:AB_2253921

This polyclonal targets RPS3A

Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 568 Conjugated (antibody)

RRID:AB_2534072

This unknown targets IgG (H+L)

Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (antibody)

RRID:AB_143165

This polyclonal targets IgG (H+L)

Anti-LAMP1 antibody - Lysosome Marker (antibody)

RRID:AB_775978

This polyclonal targets LAMP1

Anti-Puromycin Antibody, clone 12D10 (antibody)

RRID:AB_2566826

This monoclonal targets Puromycin from Streptomyces alboniger

Phospho-Akt (Ser473) Antibody (antibody)

RRID:AB_329825

This polyclonal targets Phospho-Akt (Ser473)

Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (antibody)

RRID:AB_143157

This polyclonal secondary targets IgG (H+L)

Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (antibody)

RRID:AB_331679

This polyclonal targets Phospho-S6 Ribosomal Protein (Ser235/236)

Anti-RPS3A antibody - C-terminal (antibody)

RRID:AB_2756396

This polyclonal targets RPS3A

RPL24 antibody (antibody)

RRID:AB_2181728

This polyclonal targets RPL24

RPL10A antibody (antibody)

RRID:AB_2181281

This polyclonal targets RPL10A

Recombinant Anti-mTOR (phospho S2448) antibody [EPR426(2)] (antibody)

RRID:AB_10888105

This recombinant monoclonal targets mTOR (phospho S2448)

mTOR (7C10) Rabbit mAb (antibody)

RRID:AB_2105622

This monoclonal targets mTOR

Anti-Puromycin, Alexa Fluor® 647 Conjugate Antibody (antibody)

RRID:AB_2736876

This monoclonal targets Puromycin

Lamin B2 antibody (antibody)

RRID:AB_10681013

This polyclonal targets Lamin B2 antibody

Mouse Anti-GM130 Monoclonal Antibody, Unconjugated, Clone 35 (antibody)

RRID:AB_398141

This monoclonal targets GM130

SFPQ antibody (antibody)

RRID:AB_945424

This polyclonal targets SFPQ antibody

RPL19 antibody (antibody)

RRID:AB_945305

This monoclonal targets RPL19 antibody

Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (antibody)

RRID:AB_141371

This polyclonal secondary targets IgG (H+L)

Volocity 3D Image Analysis Software (software resource)

RRID:SCR_002668

3D image analysis software to visualize, analyze and validate 3D fluorescence images from a wide range of confocal microscopy, widefield and high content screening systems. It is fully integrated for a seamless user experience.

Fiji (software resource)

RRID:SCR_002285

Software package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.

MATLAB (software resource)

RRID:SCR_001622

Multi paradigm numerical computing environment and fourth generation programming language developed by MathWorks. Allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces, and interfacing with programs written in other languages, including C, C++, Java, Fortran and Python. Used to explore and visualize ideas and collaborate across disciplines including signal and image processing, communications, control systems, and computational finance.

GraphPad Prism (software resource)

RRID:SCR_002798

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

Mouse Anti-GM130 Monoclonal Antibody, Unconjugated, Clone 35 (antibody)

RRID:AB_398141

This monoclonal targets GM130

Anti-RAB7 antibody [EPR7589] (ab137029) (antibody)

RRID:AB_2629474

This monoclonal targets RAB7

Rabbit IgG, polyclonal - Isotype Control (antibody)

RRID:AB_2631996

This polyclonal targets

Mouse Anti-Rab 5 Monoclonal antibody, Unconjugated, Clone d-11 (antibody)

RRID:AB_628191

This monoclonal targets RAB5A

Anti-GFP antibody [9F9.F9] (antibody)

RRID:AB_298911

This monoclonal targets GFP

RPL10A antibody (antibody)

RRID:AB_2181281

This polyclonal targets RPL10A

RPL24 antibody (antibody)

RRID:AB_2181728

This polyclonal targets RPL24

RPS3A antibody (antibody)

RRID:AB_2253921

This polyclonal targets RPS3A

Anti-LAMP1 antibody - Lysosome Marker (antibody)

RRID:AB_775978

This polyclonal targets LAMP1

GraphPad Prism (software resource)

RRID:SCR_002798

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

MATLAB (software resource)

RRID:SCR_001622

Multi paradigm numerical computing environment and fourth generation programming language developed by MathWorks. Allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces, and interfacing with programs written in other languages, including C, C++, Java, Fortran and Python. Used to explore and visualize ideas and collaborate across disciplines including signal and image processing, communications, control systems, and computational finance.

Volocity 3D Image Analysis Software (software resource)

RRID:SCR_002668

3D image analysis software to visualize, analyze and validate 3D fluorescence images from a wide range of confocal microscopy, widefield and high content screening systems. It is fully integrated for a seamless user experience.

Fiji (software resource)

RRID:SCR_002285

Software package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.

pHR-scFv-GCN4-sfGFP-GB1-dWPRE (plasmid)

RRID:Addgene_60907

Lamp1-RFP (plasmid)

RRID:Addgene_1817

Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (antibody)

RRID:AB_143165

This polyclonal targets IgG (H+L)

Young Adult Pigmented Xenopus Laevis (organism)

RRID:XEP_Xla100

These catalog numbers represent the same frogs: HCG- IMP- XL- FM, IMP XL FM, IMP XL M, IMP XL PB, OP IMP FM, Y XL, YXL

Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (antibody)

RRID:AB_143157

This polyclonal secondary targets IgG (H+L)

pcDNA4TO-24xGCN4_v4-kif18b-24xPP7 (plasmid)

RRID:Addgene_74928

SFPQ antibody (antibody)

RRID:AB_945424

This polyclonal targets SFPQ antibody

Goat Anti-Human VDAC2 Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_778790

This polyclonal targets Human VDAC2

Anti-Puromycin, Alexa Fluor® 647 Conjugate Antibody (antibody)

RRID:AB_2736876

This monoclonal targets Puromycin

Lamin B2 antibody (antibody)

RRID:AB_10681013

This polyclonal targets Lamin B2 antibody

Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)

RRID:AB_2534069

This polyclonal secondary targets IgG (H+L)

Recombinant Anti-mTOR (phospho S2448) antibody [EPR426(2)] (antibody)

RRID:AB_10888105

This recombinant monoclonal targets mTOR (phospho S2448)

Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (antibody)

RRID:AB_331679

This polyclonal targets Phospho-S6 Ribosomal Protein (Ser235/236)

Phospho-Akt (Ser473) Antibody (antibody)

RRID:AB_329825

This polyclonal targets Phospho-Akt (Ser473)

mTOR (7C10) Rabbit mAb (antibody)

RRID:AB_2105622

This monoclonal targets mTOR

Anti-Puromycin Antibody, clone 12D10 (antibody)

RRID:AB_2566826

This monoclonal targets Puromycin from Streptomyces alboniger

pHR-scFv-GCN4-sfGFP-GB1-dWPRE (plasmid)

RRID:Addgene_60907

pcDNA4TO-24xGCN4_v4-kif18b-24xPP7 (plasmid)

RRID:Addgene_74928

Lamp1-RFP (plasmid)

RRID:Addgene_1817

Young Adult Pigmented Xenopus Laevis (organism)

RRID:XEP_Xla100

These catalog numbers represent the same frogs: HCG- IMP- XL- FM, IMP XL FM, IMP XL M, IMP XL PB, OP IMP FM, Y XL, YXL

Mouse Anti-GM130 Monoclonal Antibody, Unconjugated, Clone 35 (antibody)

RRID:AB_398141

This monoclonal targets GM130

Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (antibody)

RRID:AB_143157

This polyclonal secondary targets IgG (H+L)

pcDNA4TO-24xGCN4_v4-kif18b-24xPP7 (plasmid)

RRID:Addgene_74928

pHR-scFv-GCN4-sfGFP-GB1-dWPRE (plasmid)

RRID:Addgene_60907

Lamp1-RFP (plasmid)

RRID:Addgene_1817

Young Adult Pigmented Xenopus Laevis (organism)

RRID:XEP_Xla100

These catalog numbers represent the same frogs: HCG- IMP- XL- FM, IMP XL FM, IMP XL M, IMP XL PB, OP IMP FM, Y XL, YXL

Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (antibody)

RRID:AB_143165

This polyclonal targets IgG (H+L)

MATLAB (software resource)

RRID:SCR_001622

Multi paradigm numerical computing environment and fourth generation programming language developed by MathWorks. Allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces, and interfacing with programs written in other languages, including C, C++, Java, Fortran and Python. Used to explore and visualize ideas and collaborate across disciplines including signal and image processing, communications, control systems, and computational finance.

Fiji (software resource)

RRID:SCR_002285

Software package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.

Volocity 3D Image Analysis Software (software resource)

RRID:SCR_002668

3D image analysis software to visualize, analyze and validate 3D fluorescence images from a wide range of confocal microscopy, widefield and high content screening systems. It is fully integrated for a seamless user experience.

GraphPad Prism (software resource)

RRID:SCR_002798

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (antibody)

RRID:AB_141371

This polyclonal secondary targets IgG (H+L)

Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)

RRID:AB_2534069

This polyclonal secondary targets IgG (H+L)

Goat Anti-Human VDAC2 Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_778790

This polyclonal targets Human VDAC2

Lamin B2 antibody (antibody)

RRID:AB_10681013

This polyclonal targets Lamin B2 antibody

SFPQ antibody (antibody)

RRID:AB_945424

This polyclonal targets SFPQ antibody

Anti-Puromycin, Alexa Fluor® 647 Conjugate Antibody (antibody)

RRID:AB_2736876

This monoclonal targets Puromycin

Anti-Puromycin Antibody, clone 12D10 (antibody)

RRID:AB_2566826

This monoclonal targets Puromycin from Streptomyces alboniger

Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (antibody)

RRID:AB_331679

This polyclonal targets Phospho-S6 Ribosomal Protein (Ser235/236)

mTOR (7C10) Rabbit mAb (antibody)

RRID:AB_2105622

This monoclonal targets mTOR

Recombinant Anti-mTOR (phospho S2448) antibody [EPR426(2)] (antibody)

RRID:AB_10888105

This recombinant monoclonal targets mTOR (phospho S2448)

Phospho-Akt (Ser473) Antibody (antibody)

RRID:AB_329825

This polyclonal targets Phospho-Akt (Ser473)

RPL10A antibody (antibody)

RRID:AB_2181281

This polyclonal targets RPL10A

Rabbit IgG, polyclonal - Isotype Control (antibody)

RRID:AB_2631996

This polyclonal targets

Anti-GFP antibody [9F9.F9] (antibody)

RRID:AB_298911

This monoclonal targets GFP

Anti-RAB7 antibody [EPR7589] (ab137029) (antibody)

RRID:AB_2629474

This monoclonal targets RAB7

Mouse Anti-Rab 5 Monoclonal antibody, Unconjugated, Clone d-11 (antibody)

RRID:AB_628191

This monoclonal targets RAB5A

Anti-LAMP1 antibody - Lysosome Marker (antibody)

RRID:AB_775978

This polyclonal targets LAMP1

RPL24 antibody (antibody)

RRID:AB_2181728

This polyclonal targets RPL24

RPL19 antibody (antibody)

RRID:AB_945305

This monoclonal targets RPL19 antibody

Anti-RPS3A antibody - C-terminal (antibody)

RRID:AB_2756396

This polyclonal targets RPS3A

RPS3A antibody (antibody)

RRID:AB_2253921

This polyclonal targets RPS3A