Tim29 is a novel subunit of the human TIM22 translocase and is involved in complex assembly and stability.
The TIM22 complex mediates the import of hydrophobic carrier proteins into the mitochondrial inner membrane. While the TIM22 machinery has been well characterised in yeast, the human complex remains poorly characterised. Here, we identify Tim29 (C19orf52) as a novel, metazoan-specific subunit of the human TIM22 complex. The protein is integrated into the mitochondrial inner membrane with it's C-terminus exposed to the intermembrane space. Tim29 is required for the stability of the TIM22 complex and functions in the assembly of hTim22. Furthermore, Tim29 contacts the Translocase of the Outer Mitochondrial Membrane, TOM complex, enabling a mechanism for transport of hydrophobic carrier substrates across the aqueous intermembrane space. Identification of Tim29 highlights the significance of analysing mitochondrial import systems across phylogenetic boundaries, which can reveal novel components and mechanisms in higher organisms.
Pubmed ID: 27554484 RIS Download
Research resources used in this publication
- Mascot (RRID:SCR_014322) (software resource)
- FlowJo (RRID:SCR_008520) (software resource)
- ImageJ (RRID:SCR_003070) (software resource)
- HeLa (RRID:CVCL_0030) (cell line)
- FreeStyle 293-F (RRID:CVCL_D603) (cell line)
- Flp-In-T-REx-293 (RRID:CVCL_U427) (cell line)
- COX IV (3E11) Rabbit mAb (RRID:AB_2085424) (antibody)
- COX IV (3E11) Rabbit mAb (RRID:AB_2085424) (antibody)
Additional research tools detected in this publication
Antibodies used in this publication
- COX IV (3E11) Rabbit mAb (RRID:AB_2085424)
- TOM70 antibody (RRID:AB_2303727)
- NDUFV2 antibody (RRID:AB_2149048)
- NDUFV1 antibody (RRID:AB_2149040)
- TIMM9 antibody (RRID:AB_945837)
- Anti-C19orf52 antibody produced in rabbit (RRID:AB_10963429)
- Anti-TIMM22 (96-110) antibody produced in rabbit (RRID:AB_1858017)
- Anti-SLC25A22 (RRID:AB_2619663)
- Anti-SLC25A6 (RRID:AB_2619664)
- Mouse Anti-SDHA Monoclonal Antibody, Unconjugated, Clone 2E3 (RRID:AB_301433)
- Tim23 (RRID:AB_398755)
- Mouse Anti-OPA1 Monoclonal Antibody, Unconjugated, Clone 18 (RRID:AB_399888)
- Cytochrome c (RRID:AB_396417)
- Monoclonal ANTI-FLAG® M2 antibody produced in mouse (RRID:AB_262044)
- Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (RRID:AB_2534078)
- Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2534069)
Associated grants
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This is a list of tools and resources that we have found mentioned in this publication.
Ambion Inc. (tool)
RRID:SCR_008406
A division of Applied Biosystems selling products for the isolation, detection, quantification, amplification, and characterization of RNA.
View all literature mentionsFISHER (tool)
RRID:SCR_009181
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RRID:AB_10963429
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RRID:AB_2619664
This polyclonal targets SLC25A6
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RRID:AB_301433
This monoclonal targets SDHA
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This monoclonal targets OPA1
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RRID:AB_396417
This monoclonal targets Cytochrome c
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RRID:AB_262044
This monoclonal targets FLAG
View all literature mentionsGoat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (antibody)
RRID:AB_2534078
This polyclonal secondary targets Rabbit IgG (H+L)
View all literature mentionsGoat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)
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View all literature mentionsMascot (software resource)
RRID:SCR_014322
A software package and server used to identify and characterize proteins from primary sequence databases using mass spectrometry data. Mascot integrates peptide mass fingerprinting, sequence querying, and MS/MS ion searching in order to search for proteins in databases like SwissProt, NCBInr, EMBL EST divisions, contaminants, and cRAP. If a license is purchased, users may: search data sets that exceed the 1200 spectrum limit of the free version; set up automated, high throughput work; add and edit proteins and quantification methods; and search a preferred collection of sequence databases. The software package works with instruments from AB Sciex, Agilent, Bruker, Jeol, Shimadzu, Thermo Scientific, and Waters.
View all literature mentionsFlowJo (software resource)
RRID:SCR_008520
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View all literature mentionsImageJ (software resource)
RRID:SCR_003070
Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsHeLa (cell line)
RRID:CVCL_0030
Cell line HeLa is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsFreeStyle 293-F (cell line)
RRID:CVCL_D603
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View all literature mentionsFlp-In-T-REx-293 (cell line)
RRID:CVCL_U427
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RRID:AB_2085424
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RRID:AB_2085424
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View all literature mentions
