Targeted inhibition of the Shroom3-Rho kinase protein-protein interaction circumvents Nogo66 to promote axon outgrowth.
Inhibitory molecules in the adult central nervous system, including NogoA, impede neural repair by blocking axon outgrowth. The actin-myosin regulatory protein Shroom3 directly interacts with Rho kinase and conveys axon outgrowth inhibitory signals from Nogo66, a C-terminal inhibitory domain of NogoA. The purpose of this study was to identify small molecules that block the Shroom3-Rho kinase protein-protein interaction as a means to modulate NogoA signaling and, in the longer term, enhance axon outgrowth during neural repair.
Pubmed ID: 26077244 RIS Download
Research resources used in this publication
- CS2 Vector Resource (RRID:SCR_007237) (material resource)
- Olympus MicroSuite Five (RRID:SCR_014425) (software resource)
- GraphPad Prism (RRID:SCR_002798) (software resource)
- CS2 Vector Resource (RRID:SCR_007237) (material resource)
- Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (RRID:AB_2576217) (antibody)
Additional research tools detected in this publication
None foundAssociated grants
- Agency: NCATS NIH HHS, United States
Id: UL1 TR000433 - Agency: NIGMS NIH HHS, United States
Id: T32 GM008353 - Agency: NCI NIH HHS, United States
Id: P30 CA046592 - Agency: NCATS NIH HHS, United States
Id: UL1TR000433 - Agency: NIA NIH HHS, United States
Id: R21 AG034264 - Agency: NIDDK NIH HHS, United States
Id: P30 DK020572 - Agency: NIA NIH HHS, United States
Id: R21AG034264
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This is a list of tools and resources that we have found mentioned in this publication.
Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_2576217
This polyclonal secondary targets IgG (H+L)
View all literature mentionsRabbit Anti-Green Fluorescent Protein (GFP) Polyclonal Antibody, Unconjugated (antibody)
RRID:AB_221569
This polyclonal targets GFP
View all literature mentionsCS2 Vector Resource (material resource)
RRID:SCR_007237
This web site has been created to provide a centralized source for CS2 and related vector information. It will be updated (on an irregular basis) with additional information, including new vector designs. CS2 vector: pCS2 is a multipurpose expression vector. Although originally designed for expressing proteins in Xenopus embryos from either injected RNA or DNA, pCS2 is also useful for high-level transient expression in a wide variety of mammalian and avian cells. It is also functional in zebrafish embryos (as DNA or RNA), and it can be used for in vitro transcription/translation (using, for example, the Promega TnT system). A number of derivatives of CS2 have been constructed that allow fusions to epitope tags and other marker proteins, as well as nuclear localization signals or the gal4 DNA binding and activation domains. In almost all cases, the same reading frames are used for the fusion vectors, to facilitate moving genes between multiple CS2 derivatives. pCS2 features: pCS2 contains a strong enhancer/promoter (simian CMV IE94) followed by a polylinker and the SV40 late polyadenlyation site. An SP6 promoter is present in the 5'' untranslated region of the mRNA from the sCMV promoter, allowing in vitro RNA synthesis of sequences cloned into the polylinker. A T7 promoter in reverse orientation between the polylinker and the SV40 polyA site for probe synthesis, as well as a second polylinker after the SV40 polyA site to provide several possible sites to linearize the vector for SP6 RNA transcription. The vector backbone is from pBluescript II KS and includes the amp resistance gene and an f1 origin for producing single stranded DNA. :Sponsors: This resource is supported by the University of Michigan. : Keywords: Vector, Resource, Expression, Protein, Xenopus, Embryo, RNA, DNA, Transient, Zebrafish, Domain, mRNA, Synthesis, Promoter,
View all literature mentionsOlympus MicroSuite Five (software resource)
RRID:SCR_014425
Imaging software for monochrome and color image acquisition and processing. It is fully integrated with Olympus microscopes, as well as third party stages and other devices, and supports various CCD and video cameras and TWAIN devices.
View all literature mentionsGraphPad Prism (software resource)
RRID:SCR_002798
Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsCS2 Vector Resource (material resource)
RRID:SCR_007237
This web site has been created to provide a centralized source for CS2 and related vector information. It will be updated (on an irregular basis) with additional information, including new vector designs. CS2 vector: pCS2 is a multipurpose expression vector. Although originally designed for expressing proteins in Xenopus embryos from either injected RNA or DNA, pCS2 is also useful for high-level transient expression in a wide variety of mammalian and avian cells. It is also functional in zebrafish embryos (as DNA or RNA), and it can be used for in vitro transcription/translation (using, for example, the Promega TnT system). A number of derivatives of CS2 have been constructed that allow fusions to epitope tags and other marker proteins, as well as nuclear localization signals or the gal4 DNA binding and activation domains. In almost all cases, the same reading frames are used for the fusion vectors, to facilitate moving genes between multiple CS2 derivatives. pCS2 features: pCS2 contains a strong enhancer/promoter (simian CMV IE94) followed by a polylinker and the SV40 late polyadenlyation site. An SP6 promoter is present in the 5'' untranslated region of the mRNA from the sCMV promoter, allowing in vitro RNA synthesis of sequences cloned into the polylinker. A T7 promoter in reverse orientation between the polylinker and the SV40 polyA site for probe synthesis, as well as a second polylinker after the SV40 polyA site to provide several possible sites to linearize the vector for SP6 RNA transcription. The vector backbone is from pBluescript II KS and includes the amp resistance gene and an f1 origin for producing single stranded DNA. :Sponsors: This resource is supported by the University of Michigan. : Keywords: Vector, Resource, Expression, Protein, Xenopus, Embryo, RNA, DNA, Transient, Zebrafish, Domain, mRNA, Synthesis, Promoter,
View all literature mentionsGoat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)
RRID:AB_2576217
This polyclonal secondary targets IgG (H+L)
View all literature mentions
