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NCI-H513

RRID:CVCL_A570

Organism

Homo sapiens

Comments

Problematic cell line: Contaminated. Shown to be a NCI-H125 derivative. Originally thought to originate with the pleural effusion of a mesothelioma in a 64 year old male patient. Part of: Cancer Cell Line Encyclopedia (CCLE) project. Part of: COSMIC cell lines project. Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00414. Microsatellite instability: Stable (MSS) (Sanger). Omics: Array-based CGH. Omics: Deep exome analysis. Omics: DNA methylation analysis. Omics: Transcriptome analysis. Genome ancestry: African=83.85%; Native American=0%; East Asian, North=2.18%; East Asian, South=0%; South Asian=2.16%; European, North=5.9%; European, South=5.9% (PubMed=30894373). Discontinued: ATCC; CRL-5830. Discontinued: DepMap; ACH-002341. DT Created: 06-06-12; Last updated: 05-07-19; Version: 20

Proper Citation

KCLB Cat# 90513, RRID:CVCL_A570

Category

Cancer cell line DT Created: 06-06-12; Last updated: 05-07-19; Version: 20

Sex

DT Created: 06-06-12; Last updated: 05-07-19; Version: 20

Synonyms

H513, H-513, NCIH513, HUT513 DT Created: 06-06-12, Last updated: 05-07-19, Version: 20

Vendor

KCLB

Cat Num

90513

Cross References

ArrayExpress; E-MTAB-3610 ATCC; CRL-5830 BioSample; SAMN03151854 CCLE; NCIH513_PLEURA Cell_Model_Passport; SIDM00114 Cosmic; 877271 Cosmic; 877408 Cosmic; 980997 Cosmic; 1032388 Cosmic; 1152503 Cosmic; 1995420 Cosmic-CLP; 1240141 DepMap; ACH-002138 DepMap; ACH-002341 GDSC; 1240141 GEO; GSM1669835 KCLB; 90513 Wikidata; Q54908072 DT Created: 06-06-12; Last updated: 05-07-19; Version: 20

Hierarchy

DT Created: 06-06-12; Last updated: 05-07-19; Version: 20

Originate from Same Individual

DT Created: 06-06-12; Last updated: 05-07-19; Version: 20

Publications that use this research resource

Loss of functional BAP1 augments sensitivity to TRAIL in cancer cells.

  • Kolluri KK
  • Elife
  • 2018 Jan 18

Literature context:


Abstract:

Malignant mesothelioma (MM) is poorly responsive to systemic cytotoxic chemotherapy and invariably fatal. Here we describe a screen of 94 drugs in 15 exome-sequenced MM lines and the discovery of a subset defined by loss of function of the nuclear deubiquitinase BRCA associated protein-1 (BAP1) that demonstrate heightened sensitivity to TRAIL (tumour necrosis factor-related apoptosis-inducing ligand). This association is observed across human early passage MM cultures, mouse xenografts and human tumour explants. We demonstrate that BAP1 deubiquitinase activity and its association with ASXL1 to form the Polycomb repressive deubiquitinase complex (PR-DUB) impacts TRAIL sensitivity implicating transcriptional modulation as an underlying mechanism. Death receptor agonists are well-tolerated anti-cancer agents demonstrating limited therapeutic benefit in trials without a targeting biomarker. We identify BAP1 loss-of-function mutations, which are frequent in MM, as a potential genomic stratification tool for TRAIL sensitivity with immediate and actionable therapeutic implications.

Funding information:
  • Cancer Research UK - A17341()
  • NINDS NIH HHS - R01NS043915(United States)
  • Wellcome - WT097452MA()
  • Wellcome Trust - 106555/Z/14/Z()
  • Wellcome Trust - WT107963AIA()