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HFF-1

RRID:CVCL_3285

Organism

Homo sapiens

Proper Citation

ATCC Cat# SCRC-1041, RRID:CVCL_3285

Reference

PMID:18415935

Category

Finite cell line

Sex

Male

Synonyms

HFF1

Vendor

ATCC

Cat Num

SCRC-1041

Cross References

BTO; BTO:0004784 CLO; CLO_0003730 ATCC; SCRC-1041 BCRJ; 0275 BioSample; SAMN03471935 Lonza; 1293

Publications that use this research resource

Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation.

  • Periz J
  • Elife
  • 2017 Mar 21

Apicomplexan actin is important during the parasite's life cycle. Its polymerization kinetics are unusual, permitting only short, unstable F-actin filaments. It has not been possible to study actin in vivo and so its physiological roles have remained obscure, leading to models distinct from conventional actin behaviour. Here a modified version of the commercially available actin-chromobody was tested as a novel tool for visualising F-actin dynamics in Toxoplasma gondii. Cb labels filamentous actin structures within the parasite cytosol and labels an extensive F-actin network that connects parasites within the parasitophorous vacuole and allows vesicles to be exchanged between parasites. In the absence of actin, parasites lack a residual body and inter-parasite connections and grow in an asynchronous and disorganized manner. Collectively, these data identify new roles for actin in the intracellular phase of the parasites lytic cycle and provide a robust new tool for imaging parasitic F-actin dynamics.