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MRC-5

RRID:CVCL_0440

Organism

Homo sapiens

Comments

Group: Vaccine production cell line. Biotechnology: Used for the production of the Human Diploid Cell Vaccine (HDCV) against rabies (Trade name: Imovax Rabies). Biotechnology: Used for the production of the Hepatitis A vaccines Avaxim, Epaxal, Havrix and Vaqta. Biotechnology: Used for the production of the live varicella virus vaccine (Trade name: Varivax). Biotechnology: Used for the production of the live zoster vaccine (Trade name: Zostavax). Characteristics: Senesces at 26 PDL (PubMed=17395773). Doubling time: ~34 hours (lot 06022011), ~1.5 days (lot 05202005), ~2 days (lot 05192003), 36 hours (lot 041392), ~5 days (lot 031098), ~38 hours (lot 04212016) (JCRB). Omics: DNA methylation analysis. Omics: Proteome analysis by 2D-DE. Omics: Proteome analysis by 2D-DE/MS. Omics: Transcriptome analysis. Misspelling: MCR-5; Occasionally. Discontinued: CLS; 300395; true. Discontinued: ECACC; 97112601; true. DT Created: 04-04-12; Last updated: 06-09-19; Version: 27

Proper Citation

RCB Cat# RCB0211, RRID:CVCL_0440

Category

Finite cell line DT Created: 04-04-12; Last updated: 06-09-19; Version: 27

Sex

DT Created: 04-04-12; Last updated: 06-09-19; Version: 27

Synonyms

MRC5, MRC 5, MRCV, MRC-V, Medical Research Council cell strain-5 DT Created: 04-04-12, Last updated: 06-09-19, Version: 27

Vendor

RCB

Cat Num

RCB0211

Cross References

BTO; BTO:0001590 CLO; CLO_0007865 CLO; CLO_0050055 EFO; EFO_0002835 MCCL; MCC:0000336 CLDB; cl3563 CLDB; cl3564 CLDB; cl3566 CLDB; cl3567 CLDB; cl3568 CLDB; cl5168 AddexBio; T0016002/4922 ATCC; CCL-171 BCRC; 60023 BCRJ; 0180 BioSample; SAMN01821580 BioSample; SAMN01821702 BioSample; SAMN01821735 BioSample; SAMN03473183 CCRID; 3111C0001CCC000044 CCRID; 3111C0002000000002 CCRID; 3131C0001000200012 CCRID; 3131C0001000200041 CCRID; 3142C0001000000032 CCRID; 3153C0001000000002 CCTCC; GDC0032 ChEMBL-Cells; CHEMBL3308499 ChEMBL-Targets; CHEMBL614181 CLS; 300395/NA ECACC; 97112601 FCS-free; 179-2-338-1-16-3 GEO; GSM248209 GEO; GSM248210 GEO; GSM520665 GEO; GSM520666 GEO; GSM520667 GEO; GSM579888 GEO; GSM579889 GEO; GSM607969 IBRC; C10313 ICLC; HL95001 IZSLER; BS CL 68 JCRB; IFO50073 JCRB; JCRB9008 KCB; KCB 89023YJ KCB; KCB 200412YJ KCLB; 10171 Lonza; 52 NCBI_Iran; C125 PRIDE; PXD001909 RCB; RCB0211 RCB; RCB0218 Wikidata; Q3273166 DT Created: 04-04-12; Last updated: 06-09-19; Version: 27

Hierarchy

DT Created: 04-04-12; Last updated: 06-09-19; Version: 27

Originate from Same Individual

DT Created: 04-04-12; Last updated: 06-09-19; Version: 27

Dynamic Palmitoylation Targets MAP6 to the Axon to Promote Microtubule Stabilization during Neuronal Polarization.

  • Tortosa E
  • Neuron
  • 2017 May 17

Literature context:


Abstract:

Microtubule-associated proteins (MAPs) are main candidates to stabilize neuronal microtubules, playing an important role in establishing axon-dendrite polarity. However, how MAPs are selectively targeted to specific neuronal compartments remains poorly understood. Here, we show specific localization of microtubule-associated protein 6 (MAP6)/stable tubule-only polypeptide (STOP) throughout neuronal maturation and its role in axonal development. In unpolarized neurons, MAP6 is present at the Golgi complex and in secretory vesicles. As neurons mature, MAP6 is translocated to the proximal axon, where it binds and stabilizes microtubules. Further, we demonstrate that dynamic palmitoylation, mediated by the family of α/β Hydrolase domain-containing protein 17 (ABHD17A-C) depalmitoylating enzymes, controls shuttling of MAP6 between membranes and microtubules and is required for MAP6 retention in axons. We propose a model in which MAP6's palmitoylation mediates microtubule stabilization, allows efficient organelle trafficking, and controls axon maturation in vitro and in situ.

Transcriptional Elongation of HSV Immediate Early Genes by the Super Elongation Complex Drives Lytic Infection and Reactivation from Latency.

  • Alfonso-Dunn R
  • Cell Host Microbe
  • 2017 Apr 12

Literature context:


Abstract:

The cellular transcriptional coactivator HCF-1 is required for initiation of herpes simplex virus (HSV) lytic infection and for reactivation from latency in sensory neurons. HCF-1 stabilizes the viral Immediate Early (IE) gene enhancer complex and mediates chromatin transitions to promote IE transcription initiation. In infected cells, HCF-1 was also found to be associated with a network of transcription elongation components including the super elongation complex (SEC). IE genes exhibit characteristics of genes controlled by transcriptional elongation, and the SEC-P-TEFb complex is specifically required to drive the levels of productive IE mRNAs. Significantly, compounds that enhance the levels of SEC-P-TEFb also potently stimulated HSV reactivation from latency both in a sensory ganglia model system and in vivo. Thus, transcriptional elongation of HSV IE genes is a key limiting parameter governing both the initiation of HSV infection and reactivation of latent genomes.

Funding information:
  • NIGMS NIH HHS - R01 GM114141()

Co-option of an endogenous retrovirus envelope for host defense in hominid ancestors.

  • Blanco-Melo D
  • Elife
  • 2017 Apr 11

Literature context:


Abstract:

Endogenous retroviral sequences provide a molecular fossil record of ancient infections whose analysis might illuminate mechanisms of viral extinction. A close relative of gammaretroviruses, HERV-T, circulated in primates for ~25 million years (MY) before apparent extinction within the past ~8 MY. Construction of a near-complete catalog of HERV-T fossils in primate genomes allowed us to estimate a ~32 MY old ancestral sequence and reconstruct a functional envelope protein (ancHTenv) that could support infection of a pseudotyped modern gammaretrovirus. Using ancHTenv, we identify monocarboxylate transporter-1 (MCT-1) as a receptor used by HERV-T for attachment and infection. A single HERV-T provirus in hominid genomes includes an env gene (hsaHTenv) that has been uniquely preserved. This apparently exapted HERV-T env could not support virion infection but could block ancHTenv mediated infection, by causing MCT-1 depletion from cell surfaces. Thus, hsaHTenv may have contributed to HERV-T extinction, and could also potentially regulate cellular metabolism.

A Portrait of the Human Organelle Proteome In Space and Time during Cytomegalovirus Infection.

  • Jean Beltran PM
  • Cell Syst
  • 2016 Oct 26

Literature context:


Abstract:

The organelles within a eukaryotic host are manipulated by viruses to support successful virus replication and spread of infection, yet the global impact of viral infection on host organelles is poorly understood. Integrating microscopy, subcellular fractionation, mass spectrometry, and functional analyses, we conducted a cell-wide study of organelles in primary fibroblasts throughout the time course of human cytomegalovirus (HCMV) infection. We used label-free and isobaric-labeling proteomics to characterize nearly 4,000 host and 100 viral proteins, then classified their specific subcellular locations over time using machine learning. We observed a global reorganization of proteins across the secretory pathway, plasma membrane, and mitochondria, including reorganization and processing of lysosomal proteins into distinct subpopulations and translocations of individual proteins between organelles at specific time points. We also demonstrate that MYO18A, an unconventional myosin that translocates from the plasma membrane to the viral assembly complex, is necessary for efficient HCMV replication. This study provides a comprehensive resource for understanding host and virus biology during HCMV pathogenesis.