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KNS-42

RRID:CVCL_0378

Organism

Homo sapiens

Comments

Part of: Cancer Cell Line Encyclopedia (CCLE) project. Part of: COSMIC cell lines project. Doubling time: 48 hours (PubMed=19365568). Microsatellite instability: Stable (MSS) (Sanger). Omics: CNV analysis. Omics: Deep exome analysis. Omics: Deep RNAseq analysis. Omics: DNA methylation analysis. Omics: SNP array analysis. Omics: Transcriptome analysis. Genome ancestry: African=0%; Native American=0.32%; East Asian, North=85.4%; East Asian, South=14.06%; South Asian=0%; European, North=0%; European, South=0.22% (PubMed=30894373). DT Created: 04-04-12; Last updated: 05-07-19; Version: 23

Proper Citation

JCRB Cat# IFO50356, RRID:CVCL_0378

Category

Cancer cell line DT Created: 04-04-12; Last updated: 05-07-19; Version: 23

Sex

DT Created: 04-04-12; Last updated: 05-07-19; Version: 23

Synonyms

KNS42 DT Created: 04-04-12, Last updated: 05-07-19, Version: 23

Vendor

JCRB

Cat Num

IFO50356

Cross References

BTO; BTO:0005859 MCCL; MCC:0000273 ArrayExpress; E-MTAB-2770 ArrayExpress; E-MTAB-3610 BCRJ; 0295 BioSample; SAMN03472787 BioSample; SAMN10987931 CCLE; KNS42_CENTRAL_NERVOUS_SYSTEM Cell_Model_Passport; SIDM00607 CGH-DB; 144-1 ChEMBL-Cells; CHEMBL3308538 ChEMBL-Targets; CHEMBL2366261 Cosmic; 687573 Cosmic; 907282 Cosmic; 2367532 Cosmic-CLP; 907282 DepMap; ACH-000622 GDSC; 907282 GEO; GSM887229 GEO; GSM888303 GEO; GSM1669995 GEO; GSM2197880 GEO; GSM2197881 GEO; GSM2197882 GEO; GSM2197883 GEO; GSM2197884 GEO; GSM2197885 JCRB; IFO50356 LiGeA; CCLE_847 LINCS_LDP; LCL-1382 Wikidata; Q54900250 DT Created: 04-04-12; Last updated: 05-07-19; Version: 23

Hierarchy

DT Created: 04-04-12; Last updated: 05-07-19; Version: 23

Originate from Same Individual

DT Created: 04-04-12; Last updated: 05-07-19; Version: 23

Publications that use this research resource

H3.3K27M mutant proteins reprogram epigenome by sequestering the PRC2 complex to poised enhancers.

  • Fang D
  • Elife
  • 2018 Jun 22

Literature context:


Abstract:

Expression of histone H3.3K27M mutant proteins in human diffuse intrinsic pontine glioma (DIPG) results in a global reduction of tri-methylation of H3K27 (H3K27me3), and paradoxically, H3K27me3 peaks remain at hundreds of genomic loci, a dichotomous change that lacks mechanistic insights. Here, we show that the PRC2 complex is sequestered at poised enhancers, but not at active promoters with high levels of H3.3K27M proteins, thereby contributing to the global reduction of H3K27me3. Moreover, the levels of H3.3K27M proteins are low at the retained H3K27me3 peaks and consequently having minimal effects on the PRC2 activity at these loci. H3K27me3-mediated silencing at specific tumor suppressor genes, including Wilms Tumor 1, promotes proliferation of DIPG cells. These results support a model in which the PRC2 complex is redistributed to poised enhancers in H3.3K27M mutant cells and contributes to tumorigenesis in part by locally enhancing H3K27me3, and hence silencing of tumor suppressor genes.

Funding information:
  • National Institutes of Health - CA204297()
  • NIGMS NIH HHS - GM107466(United States)