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HEY

RRID:CVCL_0297

Organism

Homo sapiens

Comments

Part of: Cancer Cell Line Encyclopedia (CCLE) project. Part of: COSMIC cell lines project. Part of: MD Anderson Cell Lines Project. Doubling time: 30 hours (PubMed=4016745). Microsatellite instability: Stable (MSS) (Sanger). Omics: Deep exome analysis. Omics: Deep RNAseq analysis. Omics: DNA methylation analysis. Omics: Genome sequenced. Omics: Protein expression by reverse-phase protein arrays. Omics: SNP array analysis. Omics: Transcriptome analysis. Genome ancestry: African=0.88%; Native American=0.22%; East Asian, North=0.66%; East Asian, South=0.27%; South Asian=0%; European, North=67.37%; European, South=30.59% (PubMed=30894373). DT Created: 04-04-12; Last updated: 05-07-19; Version: 24

Proper Citation

RRID:CVCL_0297

Category

Cancer cell line DT Created: 04-04-12; Last updated: 05-07-19; Version: 24

Sex

DT Created: 04-04-12; Last updated: 05-07-19; Version: 24

Synonyms

Hey DT Created: 04-04-12, Last updated: 05-07-19, Version: 24

Cross References

BTO; BTO:0002590 EFO; EFO_0006580 MCCL; MCC:0000188 ArrayExpress; E-MTAB-2706 ArrayExpress; E-MTAB-3610 BioSample; SAMN03473280 CCLE; HEY_OVARY Cell_Model_Passport; SIDM00968 ChEMBL-Cells; CHEMBL3308082 ChEMBL-Targets; CHEMBL613869 Cosmic; 1102812 Cosmic; 1524358 Cosmic-CLP; 1479988 DepMap; ACH-002140 GDSC; 1479988 GEO; GSM659377 GEO; GSM1669880 Wikidata; Q54883211 DT Created: 04-04-12; Last updated: 05-07-19; Version: 24

Hierarchy

DT Created: 04-04-12; Last updated: 05-07-19; Version: 24

Originate from Same Individual

DT Created: 04-04-12; Last updated: 05-07-19; Version: 24

Publications that use this research resource

Many si/shRNAs can kill cancer cells by targeting multiple survival genes through an off-target mechanism.

  • Putzbach W
  • Elife
  • 2017 Oct 24

Literature context:


Abstract:

Over 80% of multiple-tested siRNAs and shRNAs targeting CD95 or CD95 ligand (CD95L) induce a form of cell death characterized by simultaneous activation of multiple cell death pathways preferentially killing transformed and cancer stem cells. We now show these si/shRNAs kill cancer cells through canonical RNAi by targeting the 3'UTR of critical survival genes in a unique form of off-target effect we call DISE (death induced by survival gene elimination). Drosha and Dicer-deficient cells, devoid of most miRNAs, are hypersensitive to DISE, suggesting cellular miRNAs protect cells from this form of cell death. By testing 4666 shRNAs derived from the CD95 and CD95L mRNA sequences and an unrelated control gene, Venus, we have identified many toxic sequences - most of them located in the open reading frame of CD95L. We propose that specific toxic RNAi-active sequences present in the genome can kill cancer cells.

Funding information:
  • NCI NIH HHS - R35 CA197450()
  • NCI NIH HHS - R50 CA211271()
  • NCI NIH HHS - T32 CA009560()
  • NCI NIH HHS - T32 CA070085()