URL: http://www.addgene.org/9005
Proper Citation: RRID:Addgene_9005
Insert Name: Akt1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10051603
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: An Akt-1 cDNA was amplified by reverse transcription-coupled PCR from total HeLa cell RNA and reamplified with a 5' primer containing a Kozak sequence and sequences encoding a hemagglutinin (HA) epitope and cloned into pLNCX to give pLNCX-HA-Akt. A double-stranded oligonucleotide encoding the src myristoylation sequence was inserted 5' of the HA tag to generate pLNCX-Myr-HA-Akt.
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Source: Addgene
