URL: http://www.addgene.org/82112
Proper Citation: RRID:Addgene_82112
Insert Name: EIF4E
Organism: Homo sapiens
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:27147599
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Comments: Closed state: stop codon immediately follows insert Supporting reference: Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban MH, et al. Elife. 2017, Mar 18;6. pii: e24060. PubMed PMID 28315521
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
