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Plasmid Name
RRID:Addgene_73440 RRID Copied  
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RRID:Addgene_73440
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Plasmid Information

URL: http://www.addgene.org/73440

Proper Citation: RRID:Addgene_73440

Insert Name: PG6-vioABE-P4A6-vioC-P3A2-vioD

Organism: Pseudoalteromonas luteoviolacea strain B

Bacterial Resistance: Ampicillin

Defining Citation: PMID:27079979

Vector Backbone Description: Backbone Marker:Koffas Lab, Addgene Plasmid #49795; Backbone Size:5147; Vector Backbone:pETM6; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin

Comments: Coexpression with G6 CRISPR repressor shuts off all colored metabolite production. Coexpression with 4A6 CRISPR repressor switches carbon flux from violacein production (violet culture) to proviolacein (blue-violet culture). Coexpression with with 3A2 CRISPR repressor switches carbon flux from violacein production (violet culture) to deoxyviolacein (red-violet).

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Ratings and Alerts

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Data and Source Information

Source: Addgene