URL: http://www.addgene.org/72348
Proper Citation: RRID:Addgene_72348
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26850170
Vector Backbone Description: Backbone Size:5931; Vector Backbone:pHLmMBP-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Cloning note: For cloning information, please refer to Figure S1 of the manuscript associated with this plasmid. Mutagenesis note: Quikchange mutagenesis does not work efficiently on this family of plasmids. Citation for pHLsec from which this plasmid is derived: A time- and cost-efficient system for high-level protein production in mammalian cells A. R. Aricescu, W. Lu and E. Y. Jones Acta Cryst. D62:1243-1250 (2006) PMID:17001101 doi:10.1107/S0907444906029799
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
