URL: http://www.addgene.org/71236
Proper Citation: RRID:Addgene_71236
Insert Name: humanized dCas9-KRAB T2A Puro
Organism: S. Pyogenes
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26501517
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: Note: DNA prep concentrations may be low due to the size of this plasmid, so working with low copy protocols, higher starting volumes, or fresh transformants is recommended.
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No rating or validation information has been found for pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro.
No alerts have been found for pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro.
Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
