URL: http://www.addgene.org/69946
Proper Citation: RRID:Addgene_69946
Bacterial Resistance: Kanamycin
Vector Backbone Description: Vector Backbone:pMY200; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Comments: PCR was used to amplify a fragment containing the left T-DNA border from PBht2. Two primers were designed as follows: TRDNAS: CAAAgTTggCgTATAACATAgT, downstream of SacII site in pBht2. TLDNAAPmeI: ATTATAgTTTAAACATTCggCgTTAATTCAgTACA, upstream of T-border 5’ end contains PmeI site in pBht2. 2. Digested PCR product with PmeI and SacII, gel extraction followed by recovery of ~400 bp fragment, and pBht2 vector was digested with PmeI and SacII, gel extracted, followed by recovery of ~6 kb fragment. CIP dephosphatase treatment, ligated with PCR product, electroporate into E. coli.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
