URL: http://www.addgene.org/60524
Proper Citation: RRID:Addgene_60524
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25650551
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Comments: Note that an SfiI digest will affect the Kozak sequence that is already present upstream of the insertion site for the gene of interest. This can be addressed by adding the 3 bases 'ACC' between the 5'-SfiI site and the start codon of your gene of interest: 5'-SfiI---> ggcctctgaggcc ACC atg <---start codon
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
