URL: http://www.addgene.org/45772
Proper Citation: RRID:Addgene_45772
Insert Name: hE-cadherin/Δβ-catenin
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11381089
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Partial cDNA for human E-cadherin was provided by D. Rimm (Yale University, New Haven, CT) and subcloned into the pcDNA3 mammalian expression vector (Invitrogen). Sequence analysis revealed that the 3′ end of the gene was missing after nucleotide 2644 (according to EMBL/GenBank/DDBJ under accession number L08599). This results in a truncation of the last 35 amino acids of the E-cadherin cytoplasmic domain and, as a result, does not contain the β-catenin binding region, as defined by Stappert and Kemler 1994. The COOH terminus of this truncation mutant (E-cadherin Δ β-catenin) ends at amino acid 847 (NH3-ASLSST); the frameshift adds a single threonine residue before a stop codon is introduced.
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Source: Addgene
