URL: http://www.addgene.org/45642
Proper Citation: RRID:Addgene_45642
Insert Name: RLUC - IRES - FLUC
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9593750
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5350; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: The bicistronic reporter plasmid pcDNA3-RLUC-POLIRES-FLUC was generated by subcloning the coding sequence for firefly luciferase (FLUC) from pGEM-LUC (Promega Biotech Inc.), the coding sequence for Renilla reniformis luciferase (RLUC) from pRL-CMV (Promega) and the complete poliovirus type II Lansing 5′-untranslated region (UTR) (poliovirus internal ribosome entry site, POLIRES, nucleotides 1–737) into pcDNA3. Translation of the RLUC cistron is cap-dependent, whereas that of the FLUC cistron is directed by the poliovirus IRES and is therefore cap-independent. RLUC is between NheI and XbaI. Polio IRES is between HindIII and EcoRV. FLUC is between BamHI and XhoI. See Author's Map for more detail.
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Source: Addgene
