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Plasmid Name
RRID:Addgene_35522 RRID Copied  
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RRID:Addgene_35522
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Plasmid Information

URL: http://www.addgene.org/35522

Proper Citation: RRID:Addgene_35522

Insert Name: DNMT3B1

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:15861382

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: The multiple cloning region of the pcDNA3 vector has been modified. In the modified vector, referred to as pcDNA3/Myc, the region of the multiple cloning site located between the HindIII and NotI sites was replaced by a DNA segment containing a Kozak consensus sequence, an ATG start site and the Myc epitope sequence followed by EcoRI and BamHI restriction sites (see supplemental document). The construction of pcDNA3/Myc has been described in Chen, Z.X., et al. J Cell Biochem, 95: 902-917. The full-length cDNA for human DNMT3B1 was cloned into EcoRI and BamHI sites of pcDNA3/Myc. Constructed by Zhaoxia Chen

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Data and Source Information

Source: Addgene